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• https://orcid.org/0000-0001-9198-2828

Cleland, Timothy

Physical Scientist at the Museum Conservation Institute

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• Background And Education
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Background And Education

education and training

• Ph.D. in Molecular Paleontology, North Carolina State University , Marine, Earth and Atmospheric Sciences 2012
• B.S., Colorado State University , Geosciences 2007

awards and honors

• Smithsonian Scholarly Studies Award, conferred by Smithsonian Scholarly Studies Awards, 2020

Publications

selected publications

• article

  • Kawahara, Akito Y., Storer, Caroline G., Markee, Amanda, Heckenhauer, Jacqueline, Powell, Ashlyn, Plotkin, David, Hotaling, Scott, Cleland, Timothy P., Dikow, Rebecca B., Dikow, Torsten, Kuranishi, Ryoichi B., Messcher, Rebeccah, Pauls, Steffen U., Stewart, Russell J., Tojo, Koji, and Frandsen, Paul B. 2022. "Long-read HiFi sequencing correctly assembles repetitive heavy fibroin silk genes in new moth and caddisfly genomes." Gigabyte, 2022 1–14. https://doi.org/10.46471/gigabyte.64. 2022
  • Solazzo, Caroline, Soulat, Jean, and Cleland, Timothy P. 2021. "Creation of a peptide database of corneous beta-proteins of marine turtles for the identification of tortoiseshell: archaeological combs as case study." Royal Society Open Science 8 (2):Article 201857. https://doi.org/10.1098/rsos.201857 2021
  • Schroeter, Elena R., Cleland, Timothy P., and Schweitzer, Mary H. 2021. "Deep Time Paleoproteomics: Looking Forward." Journal of Proteome Research, https://doi.org/10.1021/acs.jproteome.1c00755. 2021
  • Cleland, Timothy P., Schroeter, Elena R., and Colleary, Caitlin. 2021. "Diagenetiforms: A new term to explain protein changes as a result of diagenesis in paleoproteomics." Journal of Proteomics 230:Article 103992. https://doi.org/10.1016/j.jprot.2020.103992 2021
  • Newsome, G. Asher and Cleland, Timothy P. 2021. "In-Line Dopant Generation for Atmospheric Pressure Ionization Mass Spectrometry." Analytical Chemistry 93 (40):13527-13533. https://doi.org/10.1021/acs.analchem.1c02400 2021
  • Cleland, Timothy P., Wang, Zehai, Wang, Bowen, Picu, Catalin R., and Vashishth, Deepak. 2021. "Mechano-chemical regulation of bat wing bones for flight." Journal of the Mechanical Behavior of Biomedical Materials https://doi.org/10.1016/j.jmbbm.2021.104809 2021
  • Cleland, Timothy P., Sarancha, Julianne J., and France, Christine A. M. 2021. "Proteomic profile of bone "collagen" extracted for stable isotopes: implications for bulk and single amino acid analyses." Rapid Communications in Mass Spectrometry 35 (6):Article e9025. https://doi.org/10.1002/rcm.9025 2021
  • Shabestari, Maziar, Shabestari, Yashar R., Landin, Maria A., Pepaj, Milaim, Cleland, Timothy P., Reseland, Janne E., and Eriksen, Erik F. 2020. "Altered protein levels in bone marrow lesions of hip osteoarthritis: Analysis by proteomics and multiplex immunoassays." International Journal of Rheumatic Diseases 23 (6):788-799. https://doi.org/10.1111/1756-185X.13843 2020
  • Early, Catherine M., Morhardt, Ashley C., Cleland, Timothy P., Milensky, Christopher M., Kavich, Gwénaëlle M., and James, Helen F. 2020. "Chemical effects of diceCT staining protocols on fluid-preserved avian specimens." PLOS One 15 (9):Article e0238783. https://doi.org/10.1371/journal.pone.0238783 2020
  • Colleary, Caitlin, Little, Nicole C., and Cleland, Timothy P. 2020. "Microwave‐assisted acid hydrolysis for whole-bone proteomics and paleoproteomics." Rapid Communications in Mass Spectrometry 34 (2):Article rcm.8568. https://doi.org/10.1002/rcm.8568 2020
  • Rowlison, Tricia, Cleland, Timothy P., Ottinger, Mary Ann, and Comizzoli, Pierre. 2020. "Novel proteomic profiling of epididymal extracellular vesicles in the domestic cat reveals proteins related to sequential sperm maturation with differences observed between normospermic and teratospermic individuals." Molecular & Cellular Proteomics 19 (12):2090-2103. https://doi.org/10.1074/mcp.RA120.002251 2020
  • Schweitzer, Mary Higby, Schroeter, Elena R., Cleland, Timothy P., and Zheng, Wenxia. 2019. "Paleoproteomics of Mesozoic dinosaurs and other Mesozoic fossils." Proteomics 19 (16):1800251. https://doi.org/10.1002/pmic.201800251 2019
  • Cleland, Timothy P., Newsome, G. Asher, and Hollinger, R. Eric. 2019. "Proteomic and direct analysis in real time mass spectrometry analysis of a Native American ceremonial hat." The Analyst 144 (24):7437-7446. https://doi.org/10.1039/c9an01557d 2019
  • Alvarez-Martin, Alba, Cleland, Timothy P., Kavich, Gwénaëlle M., Janssens, Koen, and Newsome, G. Asher. 2019. "Rapid Evaluation of the Debromination Mechanism of Eosin in Oil Paint by Direct Analysis in Real Time and Direct Infusion-Electrospray Ionization Mass Spectrometry." Analytical Chemistry 91 (16):10856-10863. https://doi.org/10.1021/acs.analchem.9b02568 2019
  • Cleland, Timothy P. and Schroeter, Elena R. 2018. "A Comparison of Common Mass Spectrometry Approaches for Paleoproteomics." Journal of Proteome Research 17 (3):936-945. https://doi.org/10.1021/acs.jproteome.7b00703 2018
  • Thomas, Corinne J., Cleland, Timothy P., Sroga, Grazyna E., and Vashishth, Deepak. 2018. "Accumulation of carboxymethyl-lysine (CML) in human cortical bone." Bone 110:128-133. https://doi.org/10.1016/j.bone.2018.01.028 2018
  • Popowich, Aleksandra K., Cleland, Timothy P., and Solazzo, Caroline. 2018. "Characterization of membrane metal threads by proteomics and analysis of a 14th c. thread from an Italian textile." Journal of Cultural Heritage 33:10-07. https://doi.org/10.1016/j.culher.2018.03.007 2018
  • Cleland, Timothy P. 2018. "Human Bone Paleoproteomics Utilizing the Single-Pot, Solid-Phase-Enhanced Sample Preparation Method to Maximize Detected Proteins and Reduce Humics." Journal of Proteome Research 17 (11):3976-3983. https://doi.org/10.1021/acs.jproteome.8b00637 2018
  • Cleland, Timothy P. 2018. "Solid Digestion of Demineralized Bone as a Method To Access Potentially Insoluble Proteins and Post-Translational Modifications." Journal of Proteome Research 17 (1):536-542. https://doi.org/10.1021/acs.jproteome.7b00670 2018
  • Schroeter, Elena R., DeHart, Caroline, Cleland, Timothy P., Zheng, Wenxia, Thomas, Paul M., Kelleher, Neil L., Bern, Marshall, and Schweitzer, Mary H. 2017. "Expansion for the Brachylophosaurus canadensis collagen I sequence and additional evidence of the preservation of Cretaceous protein." Journal of Proteome Research 16 (2):920-932. https://doi.org/10.1021/acs.jproteome.6b00873 2017
  • Cleland, Timothy P., DeHart, Caroline J., Fellers, Ryan T., VanNispen, Alexandra J., Greer, Joseph B., LeDuc, Richard D., Parker, W. R., Thomas, Paul M., Kelleher, Neil L., and Brodbelt, Jennifer S. 2017. "High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer." Journal of Proteome Research 16 (5):2072-2079. https://doi.org/10.1021/acs.jproteome.7b00043 2017
  • Thomas, Corinne J., Cleland, Timothy P., Zhang, Sheng, Gundberg, Caren M., and Vashishth, Deepak. 2017. "Identification and characterization of glycation adducts on osteocalcin." Analytical Biochemistry 525:46-53. https://doi.org/10.1016/j.ab.2017.02.011 2017
  • Schroeter, Elena R. and Cleland, Timothy P. 2016. "Glutamine deamidation: an indicator of antiquity, or preservational quality?." Rapid Communications in Mass Spectrometry 30 (2):251-255. https://doi.org/10.1002/rcm.7445 2016
  • Cleland, Timothy P., Thomas, Corinne J., Gundberg, Caren M., and Vashishth, Deepak. 2016. "Influence of carboxylation on osteocalcin detection by mass spectrometry." Rapid Communications in Mass Spectrometry 30 (19):2109-2115. https://doi.org/10.1002/rcm.7692 2016
  • Cleland, Timothy P. and Vashishth, Deepak. 2015. "Bone protein extraction without demineralization using principles from hydroxyapatite chromatography." Analytical Biochemistry 472:62-66. https://doi.org/10.1016/j.ab.2014.12.006 2015
  • Cleland, Timothy P., Schroeter, Elena R., Zamdborg, Leonid, Zheng, Wenxia, Lee, Ji Eun, Tran, John C., Bern, Marshall, Duncan, Michael B., Lebleu, Valerie S., Ahlf, Dorothy R., Thomas, Paul M., Kalluri, Raghu, Kelleher, Neil L., and Schweitzer, Mary H. 2015. "Mass spectrometry and antibody-based characterization of blood vessels from Brachylophosaurus canadensis." Journal of Proteome Research 14 (12):5252-5262. https://doi.org/10.1021/acs.jproteome.5b00675 2015
  • Schweitzer, Mary Higby, Zheng, Wenxia, Cleland, Timothy P., and Bern, Marshall. 2013. "Molecular analyses of dinosaur osteocytes supports the presence of endogenous molecules." Bone 52 (1):414-423. https://doi.org/10.1016/j.bone.2012.10.010 2013
  • Cleland, Timothy P., Voegele, Kristyn, and Schweitzer, Mary H. 2012. "Empirical evaluation of bone extraction protocols." PLoS One 7 (2):https://doi.org/10.1371/journal.pone.0031443 2012
  • Boyd, Clint A., Cleland, Timothy P., Marrero, Nico L., and Clarke, Julia A. 2011. "Exploring the effects of phylogenetic uncertainty and consensus trees on stratigraphic consistency scores: a new program and a standardized method." Cladistics 27 (1):52-60. https://doi.org/10.1111/j.1096-0031.2010.00320.x 2011
  • Cleland, Timothy P., Stoskopf, Michael K., and Schweitzer, Mary H. 2011. "Histological, chemical, and morphological reexamination of the "heart" of a small Late Cretaceous Thescelosaurus." Naturwissenschaften 98 (3):203-211. https://doi.org/10.1007/s00114-010-0760-1 2011
  • Boyd, Clint A., Cleland, Timothy P., and Novas, Fernando. 2011. "Osteogenesis, homology, and function of the intercostal plates in ornithischian dinosaurs (Tetrapoda, Sauropsida)." Zoomorphology 130 (4):305-313. https://doi.org/10.1007/s00435-011-0136-x 2011

• patent

  • Cleland, Timothy P. and Vashishth, Deepak. 2017. Bone Protein Extraction Without Demineralization, United States Patent Application 20170369526. 2017
• Li, Xuankun, Ellis, Emily, Plotkin, David, Imada, Yume, Yago, Masaya, Heckenhauer, Jacqueline, Cleland, Timothy P., Dikow, Rebecca B., Dikow, Torsten, Storer, Caroline G., Kawahara, Akito Y., and Frandsen, Paul B. 2021. "First annotated genome of a mandibulate moth, Neomicropteryx cornuta , generated using PacBio HiFi sequencing." Genome Biology and Evolution, 13, (10). https://doi.org/10.1093/gbe/evab229. 2021
• Cleland, Timothy P., Schroeter, Elena R., Feranec, Robert S., and Vashishth, Deepak. 2016. "Peptide sequences from the first Castoroides ohioensis skull and the utility of old museum collections for palaeoproteomics." Proceedings of the Royal Society B: Biological Sciences, 283, (1832). https://doi.org/10.1098/rspb.2016.0593. 2016
• Cleland, Timothy P., Schroeter, Elena R., and Schweitzer, Mary H. 2015. "Biologically and diagenetically derived peptide modifications in moa collagens." Proceedings of the Royal Society B: Biological Sciences, 282. https://doi.org/10.1098/rspb.2015.0015. 2015
• Schweitzer, Mary H., Zheng, Wenxia, Cleland, Timothy P., Goodwin, Mark B., Boatman, Elizabeth, Theil, Elizabeth, Marcus, Matthew A., and Fakra, Sirine C. 2014. "A role for iron and oxygen chemistry in preserving soft tissues, cells and molecules from deep time." Proceedings of the Royal Society B: Biological Sciences, 281, (1775). https://doi.org/10.1098/rspb.2013.2741. 2014

Contact

Location

• Museum Conservation Institute  Academic Department