The primary goal of Dr. Pukazhenthi's research program is the conservation of endangered species including ungulates, elephants and rhinos in captivity and in the wild. His research interests include understanding the fundamental reproductive biology of rare and endangered species, mitigating infertility, developing assisted reproductive technologies, gamete and embryo cryopreservation, genomics, and wildlife ecology. Dr. Pukazhenthi's team produced the world's first Persian onager and Przewalski's horse foals via artificial insemination. Dr. Pukazhenthi also studies the interactions between diet and health using multi-omics approaches to improve overall health and well-being of endangered species managed under human care. 

In addition to the above, Dr. Pukazhenthi leads conservation research on the endangered mountain tapir in Ecuador. Working alongside collaborators from Cheyenne Mountain Zoo and the Andean Bear Foundation, his team has been responsible for capture, collaring, and monitoring of several wild mountain tapirs in Ecuador. Ongoing studies hope to assist with developing a national conservation action plan for tapirs in Ecuador. 

Article

• Kliver, Sergei, Houck, Marlys L., Perelman, Polina L., Totikov, Azamat, Tomarovsky, Andrei, Dudchenko, Olga, Omer, Arina D., Colaric, Zane, Weisz, David, Aiden, Erez Lieberman, Chan, Saki, Hastie, Alex, Komissarov, Aleksey, Ryder, Oliver A., Graphodatsky, Alexander, Johnson, Warren E., Maldonado, Jesús E., Pukazhenthi, Budhan S., Marinari, Paul E., Wildt, David E., and Koepfli, Klaus-Peter. 2023. "Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes)." Journal of Heredity, https://doi.org/10.1093/jhered/esad035. 2023
• Pumpitakkul, Vichayanee, Chetruengchai, Wanna, Srichomthong, Chalurmpon, Phokaew, Chureerat, Pootakham, Wirulda, Sonthirod, Chutima, Nawae, Wanapinun, Tongsima, Sissades, Wangkumhang, Pongsakorn, Wilantho, Alisa, Utara, Yongchai, Thongpakdee, Ampika, Sanannu, Saowaphang, Maikaew, Umaporn, Khuntawee, Suphattharaphonnaphan, Changpetch, Wirongrong, Phromwat, Phairot, Raschasin, Kacharin, Sarnkhaeveerakul, Phunyaphat, Supapannachart, Pannawat, Buthasane, Wannapol, Pukazhenthi, Budhan S., Koepfli, Klaus-Peter, Suriyaphol, Prapat, Tangphatsornruang, Sithichoke et al. 2023. "Comparative genomics and genome-wide SNPs of endangered Eld’s deer provide breeder selection for inbreeding avoidance." Scientific Reports, 13, (1). https://doi.org/10.1038/s41598-023-47014-x. 2023
• Humble, Emily, Stoffel, Martin A., Dicks, Kara, Ball, Alex D., Gooley, Rebecca M., Chuven, Justin, Pusey, Ricardo, Remeithi, Mohammed Al, Koepfli, Klaus-Peter, Pukazhenthi, Budhan S., Senn, Helen, and Ogden, Rob. 2023. "Conservation management strategy impacts inbreeding and mutation load in scimitar-horned oryx." Proceedings of the National Academy of Sciences of the United States of America, 120, (18). https://doi.org/10.1073/pnas.2210756120. 2023
• Vélez, Juliana, McShea, William, Pukazhenthi, Budhan, Stevenson, Pablo, and Fieberg, John. 2023. "Implications of the scale of detection for inferring co‐occurrence patterns from paired camera traps and acoustic recorders." Conservation Biology, https://doi.org/10.1111/cobi.14218. 2023
• Brown, Megan E., Pukazhenthi, Budhan, Olsen, Glenn H., Crowe, Chris, Lynch, Warren, Songsasen, Nucharin, and Wildt, David E. 2023. "Low estradiol production of non-laying whooping cranes (Grus americana) is associated with the failure of small follicles to enter follicular hierarchy." General and comparative endocrinology, 338. https://doi.org/10.1016/j.ygcen.2023.114280. 2023
• Corder, Molly L., Petricoin, Emanuel F., Li, Yue, Cleland, Timothy P., DeCandia, Alexandra L., Aguirre, A. A., and Pukazhenthi, Budhan S. 2023. "Metabolomic profiling implicates mitochondrial and immune dysfunction in disease syndromes of the critically endangered black rhinoceros (Diceros bicornis)." Scientific Reports, 13, (1). https://doi.org/10.1038/s41598-023-41508-4. 2023
• Gooley, Rebecca M., Dicks, Kara L., Ferrie, Gina M., Lacy, Robert C., Ballou, Jonathan D., Callicrate, Taylor, Senn, Helen, Koepfli, Klaus-Peter, Edwards, Cody W., and Pukazhenthi, Budhan S. 2022. "Applying genomics to metapopulation management in North American insurance populations of southern sable antelope (Hippotragus niger niger) and addra gazelle (Nanger dama ruficollis)." Global Ecology and Conservation, 33. https://doi.org/10.1016/j.gecco.2021.e01969. 2022
• Thompson, Riley E., Meyers, Mindy A., Veeramachaneni, D. N. R., Pukazhenthi, Budhan S., and Hollinshead, Fiona K. 2022. "Equine Oviductal Organoid Generation and Cryopreservation." Methods and Protocols, 5, (3). https://doi.org/10.3390/mps5030051. 2022
• Alvarez‐Estape, Marina, Fontsere, Claudia, Serres‐Armero, Aitor, Kuderna, Lukas F. K., Dobrynin, Pavel, Guidara, Héla, Pukazhenthi, Budhan S., Koepfli, Klaus‐Peter, Marques‐Bonet, Tomas, Moreno, Eulalia, and Lizano, Esther. 2022. "Insights from the rescue and breeding management of Cuvier's gazelle ( Gazella cuvieri ) through whole‐genome sequencing." Evolutionary Applications, 15, (3) 351–364. https://doi.org/10.1111/eva.13336. 2022
• Huffmeyer, Audra A., Pukazhenthi, Budhan S., and Wayne, Robert K. 2021. "Differential gene expression patterns in spermatozoa from teratospermic and normospermic domestic cats." Animal Reproduction Science, 226. https://doi.org/10.1016/j.anireprosci.2021.106698. 2021
• Novaes, M. A. S., Lima, L. F., Sa, N. A. R., Ferreira, A. C. A., Paes, V. M., Souza, J. F., Alves, B. G., Gramosa, N. V., Torres, C. A. A., Pukazhenthi, Budhan S., Gastal, E. L., and Figueiredo, J. R. 2021. "Impact of ethanol and heat stress-dependent effect of ultra-diluted Arnica montana 6 cH on in vitro embryo production in cattle." Theriogenology, 162 105–110. https://doi.org/10.1016/j.theriogenology.2020.12.026. 2021
• Conley, A. J., Loux, S. C., Legacki, E. L., Stoops, M. A., Pukazhenthi, Budhan, Brown, Janine L., Sattler, R., French, H. M., Tibary, A., and Robeck, T. R. 2021. "The steroid metabolome of pregnancy, insights into the maintenance of pregnancy and evolution of reproductive traits." Molecular and cellular endocrinology, 528. https://doi.org/10.1016/j.mce.2021.111241. 2021
• Thompson, Riley E., Premanandan, Christopher, Pukazhenthi, Budhan S., and Whitlock, Brian K. 2020. "A review of in vivo and in vitro studies of the mare endometrium." Animal Reproduction Science, 222 Article 106605. https://doi.org/10.1016/j.anireprosci.2020.106605. 2020
• Thompson, Riley E., Haefele, Holly J., O'Neil, Daniel P., and Pukazhenthi, Budhan S. 2020. "Bilateral Intratesticular Spermatoceles in a Critically Endangered Dama Gazelle ( Nanger dama mhorr )." Case Reports in Veterinary Medicine, 2020 1–3. https://doi.org/10.1155/2020/8810770. 2020
• Humble, Emily, Dobrynin, Pavel, Senn, Helen, Chuven, Justin, Scott, Alan F., Mohr, David W., Dudchenko, Olga, Omer, Arina D., Colaric, Zane, Lieberman Aiden, Erez, Al Dhaheri, Shaikha Salem, Wildt, David, Oliaji, Shireen, Tamazian, Gaik, Pukazhenthi, Budhan, Ogden, Rob, and Koepfli, Klaus-Peter. 2020. "Chromosomal-level genome assembly of the scimitar-horned oryx: Insights into diversity and demography of a species extinct in the wild." Molecular Ecology Resources, https://doi.org/10.1111/1755-0998.13181. 2020
• Gooley, Rebecca M., Tamazian, Gaik, Castaneda-Rico, Susette, Murphy, Katherine R., Dobrynin, Pavel, Ferrie, Gina M., Haefele, Holly, Maldonado, Jesus E., Wildt, David E., Pukazhenthi, Budhan S., Edwards, Cody W., and Koepfli, Klaus-Peter. 2020. "Comparison of genomic diversity and structure of sable antelope (Hippotragus niger) in zoos, conservation centers, and private ranches in North America." Evolutionary Applications, 13, (8) 2143–2154. https://doi.org/10.1111/eva.12976. 2020
• Pukazhenthi, Budhan, Songsasen, Nucharin, Brown, Janine, Comizzoli, Pierre, Crosier, Adrienne, Hagedorn, Mary, Gratwicke, Brian, Pitt, William, and Monfort, Steven. 2020. "David E. Wildt-An Inspiring Leader in the Conservation of Wild Species." Journal of Heredity, 111, (4) 414–416. https://doi.org/10.1093/jhered/esaa013. 2020
• Thompson, Riley E., Johnson, Aime K., Dini, Pouya, Turco, Margherita Y., Prado, Tulio M., Premanandan, Christopher, Burton, Graham J., Ball, Barry A., Whitlock, Brian K., and Pukazhenthi, Budhan S. 2020. "Hormone-responsive organoids from domestic mare and endangered Przewalski's horse endometrium." Reproduction, 160, (6) 819–831. https://doi.org/10.1530/REP-20-0266. 2020
• Pennington, Parker M., Splan, Rebecca K., Jacobs, Robert D., Chen, Yong, Singh, Ram P., Li, Yue, Gucek, Marjan, Wagner, Ashley L., Freeman, Elizabeth W., and Pukazhenthi, Budhan S. 2020. "Influence of Metabolic Status and Diet on Early Pregnant Equine Histotroph Proteome: Preliminary Findings." Journal of Equine Veterinary Science, 88 Article 102938. https://doi.org/10.1016/j.jevs.2020.102938. 2020
• Thompson, Riley E., Brown, Megan E., Helmick, Kelly, Whitlock, Brian K., and Pukazhenthi, Budhan S. 2020. "Persian onager (Equus hemionus onager) endometrial explant cryopreservation and in vitro culture." Animal Reproduction Science, 217 Article 106459. https://doi.org/10.1016/j.anireprosci.2020.106459. 2020
• Wildt, David, Miller, Philip, Koepfli, Klaus-Peter, Pukazhenthi, Budhan, Palfrey, Katy, Livingston, Gavin, Beetem, Dan, Shurter, Stephen, Gregory, Jimmy, Takács, Michael, and Snodgrass, Kelley. 2019. "Breeding Centers, Private Ranches, and Genomics for Creating Sustainable Wildlife Populations." Bioscience, 69, (11) 928–943. https://doi.org/10.1093/biosci/biz091. 2019
• Thompson, Riley E., Johnson, Aime K., Prado, Tulio M., Premanandan, Christopher, Brown, Megan E., Whitlock, Brian K., and Pukazhenthi, Budhan S. 2019. "Dimethyl sulfoxide maintains structure and function of cryopreserved equine endometrial explants." Cryobiology, 91 90–96. https://doi.org/10.1016/j.cryobiol.2019.10.006. 2019
• Singh, Ram Pratap, Escobar, Enrique, Wildt, David E., Patel, Seema, Costa, Guilherme M. J., and Pukazhenthi, Budhan. 2019. "Effect of sphingosine-1-phosphate on cryopreserved sheep testicular explants cultured in vitro." Theriogenology, 128 184–192. https://doi.org/10.1016/j.theriogenology.2019.01.038. 2019
• Gibson, Keylie M., Nguyen, Bryan N., Neumann, Laura M., Miller, Michele, Buss, Peter, Daniels, Savel, Ahn, Michelle J., Crandall, Keith A., and Pukazhenthi, Budhan. 2019. "Gut microbiome differences between wild and captive black rhinoceros - implications for rhino health." Scientific Reports, 9 7570. https://doi.org/10.1038/s41598-019-43875-3. 2019
• Putman, Sarah B., Brown, Janine L., Saffoe, Craig, Franklin, Ashley D., and Pukazhenthi, Budhan S. 2019. "Linkage between fecal androgen and glucocorticoid metabolites, spermaturia, body weight and onset of puberty in male African lions (Panthera leo)." PloS One, 14, (7) 1–16. https://doi.org/10.1371/journal.pone.0217986. 2019
• Koepfli, Klaus-Peter, Tamazian, Gaik, Wildt, David E., Dobrynin, Pavel, Kim, Changhoon, Frandsen, Paul B., Godinho, Raquel, Yurchenko, Andrey A., Komissarov, Aleksey, Krasheninnikova, Ksenia, Kliver, Sergei, Kolchanova, Sofia, Gonçalves, Margarida, Carneiro, Miguel, Vaz Pinto, Pedro, Ferrand, Nuno, Maldonado, Jesús E., Ferrie, Gina M., Chemnick, Leona, Ryder, Oliver A., Johnson, Warren E., Comizzoli, Pierre, O'Brien, Stephen J., and Pukazhenthi, Budhan S. 2019. "Whole Genome Sequencing and Re-sequencing of the Sable Antelope (Hippotragus niger): A Resource for Monitoring Diversity in ex Situ and in Situ Populations." G3 (Bethesda, Md.), 9, (6) 1785–1793. https://doi.org/10.1534/g3.119.400084. 2019
• Malo, Clara, Crichton, Elizabeth G., Morrell, Jane M., Pukazhenthi, Budhan S., Johannisson, Anders, Splan, Rebecca, and Skidmore, Julian A. 2018. "Colloid centrifugation of fresh semen improves post-thaw quality of cryopreserved dromedary camel spermatozoa." Animal Reproduction Science, 192 28–34. https://doi.org/10.1016/j.anireprosci.2018.02.005. 2018
• Brown, Megan E., Singh, Ram P., Pukazhenthi, Budhan, Keefer, Carol L., and Songsasen, Nucharin. 2018. "Cryopreservation effects on sperm function and fertility in two threatened crane species." Cryobiology, 82 148–154. https://doi.org/10.1016/j.cryobiol.2018.01.010. 2018
• Jacobs, R. D., Ealy, A. D., Pennington, P. M., Pukazhenthi, Budhan S., Warren, L. K., Wagner, A. L., Johnson, A. K., Hess, T. M., Knight, J. W., and Splan, R. K. 2018. "Dietary Supplementation of Algae-derived Omega-3 Fatty Acids Influences Endometrial and Conceptus Transcript Profiles in Mares." Journal of Equine Veterinary Science, 62 66–75. https://doi.org/10.1016/j.jevs.2017.08.001. 2018
• Wojtusik, Jessye, Wang, Yan, and Pukazhenthi, Budhan S. 2018. "Pretreatment with cholesterol-loaded cyclodextrins prevents loss of motility associated proteins during cryopreservation of addra gazelle (Nanger dama ruficollis) spermatozoa." Cryobiology, 81 74–80. https://doi.org/10.1016/j.cryobiol.2018.02.007. 2018
• Wojtusik, Jessye, Brown, Janine L., and Pukazhenthi, Budhan S. 2017. "Non-invasive hormonal characterization of the ovarian cycle, pregnancy, and seasonal anestrus of the female addra gazelle (Nanger dama ruficollis)." Theriogenology, 95 96–104. https://doi.org/10.1016/j.theriogenology.2017.02.019. 2017
• Vansandt, Lindsey M., Livesay, Janelle L., Dickson, Melissa Joy, Li, Lei, Pukazhenthi, Budhan S., and Keefer, Carol L. 2016. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology, 86, (4) 1022–1035. https://doi.org/10.1016/j.theriogenology.2016.03.031. 2016
• Crichton, Elizabeth G., Malo, Clara, Pukazhenthi, Budhan S., Nagy, Peter, and Skidmore, Julian A. 2016. "Evaluation of cholesterol- treated dromedary camel sperm function by heterologous IVF and AI." Animal Reproduction Science, 174 20–28. https://doi.org/10.1016/j.anireprosci.2016.08.013. 2016
• Panyaboriban, Saritvich, Pukazhenthi, Budhan S., Brown, Megan E., Crowe, Chris, Lynch, Warren E., Singh, Ram P., Techakumphu, Mongkol, and Songsasen, Nucharin. 2016. "Influence of cooling and thawing conditions and cryoprotectant concentration on frozen-thawed survival of white-naped crane (Antigone vipio) spermatozoa." Cryobiology, 73, (2) 209–215. https://doi.org/10.1016/j.cryobiol.2016.07.009. 2016
• Wojtusik, Jessye, Pennington, Parker, Songsasen, Nucharin, Padilla, Luis R., Citino, Scott B., and Pukazhenthi, Budhan S. 2016. "Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival." Cryobiology, 73, (3) 388–395. https://doi.org/10.1016/j.cryobiol.2016.08.012. 2016
• Panyaboriban, Saritvich, Singh, Ram Pratap, Songsasen, Nucharin, Padilla, Luis, Brown, Janine, Reed, Dolores, Techakumphu, Mongkol, and Pukazhenthi, Budhan S. 2016. "Reproductive seasonality and sperm cryopreservation in the male tufted deer (Elaphodus cephalophus)." Theriogenology, 86, (4) 914–923. https://doi.org/10.1016/j.theriogenology.2016.03.014. 2016
• Pukazhenthi, Budhan S. 2016. "Saving wild ungulate diversity through enhanced management and sperm cryopreservation." Reproduction Fertility and Development, 28, (8) 1133–1144. https://doi.org/10.1071/RD15412. 2016
• Malo, C., Morrell, J. M., Crichton, E. G., Pukazhenthi, Budhan S., and Skidmore, J. A. 2016. "Use of colloid single layer centrifugation for dromedary camel semen: Effect of initial dilution and comparison of two colloids on sperm quality parameters." Animal Reproduction Science, 169 123. https://doi.org/10.1016/j.anireprosci.2016.03.068. 2016
• Jacobs, R. D., Ealy, A. D., Pennington, P. M., Pukazhenthi, Budhan S., Warren, L. K., Wagner, A. L., Johnson, A. K., Hess, T. M., and Splan, R. K. 2015. "130 Effect of dietary omega-3 supplementation on equine endometrial and embryonic gene expression." Journal of Equine Veterinary Science, 35, (5) 438–439. https://doi.org/10.1016/j.jevs.2015.03.142. 2015
• Burnik Šturm, Martina, Pukazhenthi, Budhan S., Reed, Dolores, Ganbaatar, Oyunsaikhan, Sušnik, Stane, Haymerle, Agnes, Voigt, Christian C., and Kaczensky, Petra. 2015. "A protocol to correct for intra- and interspecific variation in tail hair growth to align isotope signatures of segmentally cut tail hair to a common time line: Aligning isotope signatures of hair segments to the correct time line." Rapid Communications in Mass Spectrometry, 29, (11) 1047–1054. https://doi.org/10.1002/rcm.7196. 2015
• Putman, Sarah B., Brown, Janine L., Franklin, Ashley D., Schneider, Emily C., Boisseau, Nicole P., Asa, Cheryl S., and Pukazhenthi, Budhan S. 2015. "Characterization of Ovarian Steroid Patterns in Female African Lions (Panthera leo), and the Effects of Contraception on Reproductive Function." PloS One, 10, (10). https://doi.org/10.1371/journal.pone.0140373. 2015
• Crichton, Elizabeth G., Pukazhenthi, Budhan S., Billah, M., and Skidmore, Julian A. 2015. "Cholesterol addition aids the cryopreservation of dromedary camel (Camelus dromedarius) spermatozoa." Theriogenology, 83, (2) 168–174. https://doi.org/10.1016/j.theriogenology.2014.09.005. 2015
• Daigle, C. L., Brown, Janine L., Carlstead, K., Pukazhenthi, Budhan S., Freeman, E. W., and Snider, R. J. 2015. "Multi-institutional survey of social, management, husbandry and environmental factors for the SSP African lion Panthera leo population: examining the effects of a breeding moratorium in relation to reproductive success." International Zoo Yearbook, 49, (1) 198–213. https://doi.org/10.1111/izy.12073. 2015
• Stewart, Rosemary A., Crosier, Adrienne E., Pelican, Katharine M., Pukazhenthi, Budhan S., Sitzmann, Brandon D., Porter, Tom E., Wildt, David E., Ottinger, Mary Ann, and Howard, JoGayle. 2015. "Progestin priming before gonadotrophin stimulation and AI improves embryo development and normalises luteal function in the cat." Reproduction Fertility and Development, 27, (2) 360–371. https://doi.org/10.1071/RD13274. 2015
• Pukazhenthi, Budhan S., Nagashima, Jennifer B., Travis, Alexander J., Costa, Guilherme M., Escobar, Enrique N., França, Luiz R., and Wildt, David E. 2015. "Slow freezing, but not vitrification supports complete spermatogenesis in cryopreserved, neonatal sheep testicular xenografts." PloS One, 10, (4). https://doi.org/10.1371/journal.pone.0123957. 2015
• Pukazhenthi, Budhan S., Johnson, Aime, Guthrie, H. David, Songsasen, Nucharin, Padilla, Luis R., Wolfe, Barbara A., da Silva, Marco Coutinho, Alvarenga, Marco A., and Wildt, David E. 2014. "Improved Sperm Cryosurvival in Diluents Containing Amides versus Glycerol in the Przewalski's Horse (Equus ferus przewalskii)." Cryobiology, 68, (2) 205–214. https://doi.org/10.1016/j.cryobiol.2014.01.013. 2014
• Pukazhenthi, Budhan S., Quse, Viviana, Hoyer, Mark, Gastelaars, Heleen Van Engeldorp, and Brown, Janine L. 2013. "A review of the reproductive biology and breeding management of tapirs (Tapirus sp.)." Integrative Zoology, 8, (1 (special issue)) 18–34. (On tapir ecology, evolution and conservation: what we know and future perspectives - Part II) https://doi.org/10.1111/j.1749-4877.2012.12008.x. 2013
• Schook, Mandi W., Wildt, David E., Weiss, Rachael B., Wolfe, Barbara A., Archibald, Kate E., and Pukazhenthi, Budhan S. 2013. "Fundamental Studies of the Reproductive Biology of the Endangered Persian Onager (Equus hemionus onager) Result in First Wild Equid Offspring from Artificial Insemination." Biology of reproduction, 89, (2) 41. https://doi.org/10.1095/biolreprod.113.110122. 2013
• Kiso, Wendy K., Selvaraj, Vimal, Nagashima, Jennifer B., Asano, Atsushi, Brown, Janine L., Schmitt, Dennis L., Leszyk, John, Travis, Alexander J., and Pukazhenthi, Budhan S. 2013. "Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality." PLoS ONE, 8, (8) e71033. https://doi.org/10.1371/journal.pone.0071033. 2013
• Jewgenow, K., Pukazhenthi, Budhan S., and Schoen, J. 2012. "Analysis of Sertoli cell efficiency allows the differentiation between two fundamentally different forms of feline teratospermia." Theriogenology, 79, (2) 261–266. https://doi.org/10.1016/j.theriogenology.2012.08.014. 2012
• Vick, Mandi,M., Wildt, David E., Turner, Jessica B., Palme, Rupert, Wolfe, Barbara A., and Pukazhenthi, Budhan S. 2012. "Glucocorticoid response to changes in enclosure size and human proximity in the Persian onager (Equus hemionus onager)." Stress, 15, (1) 52–61. https://doi.org/10.3109/10253890.2011.578267. 2012
• Vansandt, L. M., Pukazhenthi, Budhan S., and Keefer, C. L. 2012. "Molecular Markers of Spermatogonial Stem Cells in the Domestic Cat." Reproduction in Domestic Animals, 47, (Supplement S6) 256–260. https://doi.org/10.1111/rda.12079. 2012
• Stewart, Rosemary A., Pelican, Katharine M., Crosier, Adrienne E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, Mary Ann, and Howard, JoGayle. 2012. "Oral Progestin Priming Increases Ovarian Sensitivity to Gonadotropin Stimulation and Improves Luteal Function in the Cat." Biology of reproduction, 87, (6) 137. https://doi.org/10.1095/biolreprod.112.104190. 2012
• Kiso, Wendy K., Asano, Atsushi, Travis, Alexander J., Schmitt, Dennis L., Brown, Janine L., and Pukazhenthi, Budhan S. 2012. "Pretreatment of Asian elephant (Elephas maximus) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4°C improves cryosurvival." Reproduction, Fertility and Development, 24, (8) 1134–1142. https://doi.org/10.1071/RD11266. 2012
• Pukazhenthi, Budhan S., Togna, Gina Della, Padilla, Luis R., Smith, Diorene, Sanchez, Carlos R., Pelican, Katey, and Sanjur, Oris I. 2011. "Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)." Journal of Andrology, 32, (3) 260–270. https://doi.org/10.2164/jandrol.110.011833. 2011
• Kiso, Wendy K., Brown, Janine L., Siewerdt, Frank, Schmitt, Dennis L., Olson, Deborah, Crichton, Elizabeth G., and Pukazhenthi, Budhan S. 2011. "Liquid Semen Storage in Elephants (Elephas maximus and Loxodonta africana): Species Differences and Storage Optimization." Journal of andrology, 32, (4) 420–431. https://doi.org/10.2164/jandrol.110.011460. 2011
• Comizzoli, Pierre, Pukazhenthi, Budhan S., and Wildt, David E. 2011. "The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model." Human Reproduction, 26, (8) 2165–2177. https://doi.org/10.1093/humrep/der176. 2011
• Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E., and Pukazhenthi, Budhan S. 2010. "Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus)." Theriogenology, 73, (1) 112–119. https://doi.org/10.1016/j.theriogenology.2009.08.007. 2010
• Pelican, K. M., Spindler, Rebecca E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, M. A., and Howard, JoGayle. 2010. "Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat." Biology of Reproduction, 83, (4) 558–567. https://doi.org/10.1095/biolreprod.109.083360. 2010
• Songsasen, Nucharin, Fickes, A., Pukazhenthi, Budhan S., and Wildt, David E. 2009. "Follicular morphology, oocyte diameter and localization of fibroblast growth factors in the domestic dog ovary." Reproduction in Domestic Animals (Supplement 2), 44 65–70. 2009
• Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes." Reproduction in Domestic Animals, 44 269–274. 2009
• Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan S., and Songsasen, Nucharin. 2009. "Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health." Molecular reproduction and development, 77, (5) 397–409. https://doi.org/10.1002/mrd.21137. 2009
• Schoen, J., Neumann, S., Wildt, David E., Pukazhenthi, Budhan S., and Jewgenow, K. 2009. "Localization of estrogen receptors in the epididymis during sexual maturation of the domestic cat." Reproduction in Domestic Animals, 44 294–301. 2009
• Godard, N. M., Pukazhenthi, Budhan S., Wildt, David E., and Comizzoli, Pierre. 2009. "Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model." Fertility and sterility, 91, (5) 2051–2060. https://doi.org/10.1016/j.fertnstert.2008.05.069. 2009
• Jewgenow, Katarina, Neubauer, Katrin, Blottner, Steffen, Schon, Jennifer, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "Reduced Germ Cell Apoptosis During Spermatogenesis in the Teratospermic Domestic Cat." Journal of andrology, 30, (4) 460–468. https://doi.org/10.2164/jandrol.108.006726. 2009
• Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2008. "Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat." Molecular reproduction and development, 75 345–354. 2008
• Crosier, Adrienne E., Henghali, Josephine N., Howard, JoGayle, Pukazhenthi, Budhan S., Terrell, Kimberly A., Marker, Laurie L., and Wildt, David E. 2008. "Improved Quality of Cryopreserved Cheetah (Acinonyx jubatus) Spermatozoa after Centrifugation Through Accudenz." Journal of Andrology, 30, (3) 298. https://doi.org/10.2164/jandrol.108.006239. 2008
• Baudi, D. L. K., Jewgenow, K., Pukazhenthi, Budhan S., Spercoski, K. M., Santos, A. S., Reghelin, A. L. S., Candido, M. V., Javorouski, M. L., Muller, G., and Morais, R. N. 2008. "Influence of cooling rate on the ability of frozen-thaw spermatozoa to bind to heterologous zona pellucida as assessed by competitive in vitro binding assays in the Ocelot (Leopardus pardalis) and Tigrina (Leopardus tigrinus)." Theriogenology, 69 204–211. 2008
• Pukazhenthi, Budhan S., Santymire, Rachel, Crosier, Adrienne E., Howard, JoGayle, and Wildt, David E. 2007. "Challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival." Society for Reproduction and Fertility (Supplement), 65 433–446. 2007
• Kim, Y., Selvaraj, V., Pukazhenthi, Budhan S., and Travis, A. J. 2007. "Effect of donor age on success of spermatogenesis in feline testis xenografts." Reproduction, Fertility, and Development, 19, (7) 869–876. 2007
• Crosier, Adrienne E., Marker, Laurie, Howard, JoGayle, Pukazhenthi, Budhan S., Henghali, Josephine N., and Wildt, David E. 2007. "Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season, and captivity." Reproduction, Fertility, and Development, 19 370–382. 2007

Chapter

• Wildt, David E., Howard, JoGayle, Pelican, K., Brown, J., and Pukazhenthi, Budhan S. 2009. "Contributions of reproductive science to wild felid conservation." In Iberian Lynx Ex-situ Conservation: An interdisciplinary approach.. Breitenmoser, U., editor. Madrid, Spain: Fundación Biodiversidad. 2009