education and training
- B. V.Sc. Bachelor of Veterinary Science, Madras Veterinary College, India
- M.S., University of Maryland, College Park
- Ph.D., University of Maryland, College Park
Pukazhenthi, Budhan
Fellowship Advisor
National Zoological Park staff
Ungulate reproduction and conservation; gamete biology (sperm, eggs and embryos); cryobiology; assisted reproduction in endangered species, in situ conservation and training
Background And Education
awards and honors
Research And Grants
co-principal investigator on
investigator on
- Influence of Ex Situ Management on Gut Microbiota of the Southern Black Rhinoceros awarded by The International Rhino Foundation 2019 -
- Cryobiology of Asian Elephant Sperm - Impact of Seminal Plasma on Motility and Development of Cryopreservation Techniques awarded by Feld Entertainment, Inc
- MicroRNAs as Novel Biomarkers in Maternal Serum and Urine awarded by Morris Animal Foundation
- Role of Gut Microbiota in Health and Disease Sensitivity of the Black Rhinoceros awarded by The International Rhino Foundation
- Understanding the Role of Gut Microbiota in Health and Disease Sensitivity of the Critically Endangered Black Rhinoceros awarded by Morris Animal Foundation
Publications
selected publications
-
article
- Huffmeyer, Audra A., Pukazhenthi, Budhan S., and Wayne, Robert K. 2021. "Differential gene expression patterns in spermatozoa from teratospermic and normospermic domestic cats." Animal Reproduction Science 226:https://doi.org/10.1016/j.anireprosci.2021.106698 2021
- Novaes, M. A. S., Lima, L. F., Sa, N. A. R., Ferreira, A. C. A., Paes, V. M., Souza, J. F., Alves, B. G., Gramosa, N. V., Torres, C. A. A., Pukazhenthi, Budhan S., Gastal, E. L., and Figueiredo, J. R. 2021. "Impact of ethanol and heat stress-dependent effect of ultra-diluted Arnica montana 6 cH on in vitro embryo production in cattle." Theriogenology 162:105-110. https://doi.org/10.1016/j.theriogenology.2020.12.026 2021
- Thompson, Riley E., Premanandan, Christopher, Pukazhenthi, Budhan S., and Whitlock, Brian K. 2020. "A review of in vivo and in vitro studies of the mare endometrium." Animal Reproduction Science 222:Article 106605. https://doi.org/10.1016/j.anireprosci.2020.106605 2020
- Thompson, Riley E., Haefele, Holly J., O'Neil, Daniel P., and Pukazhenthi, Budhan S. 2020. "Bilateral Intratesticular Spermatoceles in a Critically Endangered Dama Gazelle ( Nanger dama mhorr )." Case Reports in Veterinary Medicine 2020:1-3. https://doi.org/10.1155/2020/8810770 2020
- Humble, Emily, Dobrynin, Pavel, Senn, Helen, Chuven, Justin, Scott, Alan F., Mohr, David W., Dudchenko, Olga, Omer, Arina D., Colaric, Zane, Lieberman Aiden, Erez, Al Dhaheri, Shaikha Salem, Wildt, David, Oliaji, Shireen, Tamazian, Gaik, Pukazhenthi, Budhan, Ogden, Rob, and Koepfli, Klaus-Peter. 2020. "Chromosomal-level genome assembly of the scimitar-horned oryx: Insights into diversity and demography of a species extinct in the wild." Molecular Ecology Resources https://doi.org/10.1111/1755-0998.13181 2020
- Gooley, Rebecca M., Tamazian, Gaik, Castaneda-Rico, Susette, Murphy, Katherine R., Dobrynin, Pavel, Ferrie, Gina M., Haefele, Holly, Maldonado, Jesus E., Wildt, David E., Pukazhenthi, Budhan S., Edwards, Cody W., and Koepfli, Klaus-Peter. 2020. "Comparison of genomic diversity and structure of sable antelope (Hippotragus niger) in zoos, conservation centers, and private ranches in North America." Evolutionary Applications 13 (8):2143-2154. https://doi.org/10.1111/eva.12976 2020
- Pukazhenthi, Budhan, Songsasen, Nucharin, Brown, Janine, Comizzoli, Pierre, Crosier, Adrienne, Hagedorn, Mary, Gratwicke, Brian, Pitt, William, and Monfort, Steven. 2020. "David E. Wildt-An Inspiring Leader in the Conservation of Wild Species." Journal of Heredity 111 (4):414-416. https://doi.org/10.1093/jhered/esaa013 2020
- Thompson, Riley E., Johnson, Aime K., Dini, Pouya, Turco, Margherita Y., Prado, Tulio M., Premanandan, Christopher, Burton, Graham J., Ball, Barry A., Whitlock, Brian K., and Pukazhenthi, Budhan S. 2020. "Hormone-responsive organoids from domestic mare and endangered Przewalski's horse endometrium." Reproduction 160 (6):819-831. https://doi.org/10.1530/REP-20-0266 2020
- Pennington, Parker M., Splan, Rebecca K., Jacobs, Robert D., Chen, Yong, Singh, Ram P., Li, Yue, Gucek, Marjan, Wagner, Ashley L., Freeman, Elizabeth W., and Pukazhenthi, Budhan S. 2020. "Influence of Metabolic Status and Diet on Early Pregnant Equine Histotroph Proteome: Preliminary Findings." Journal of Equine Veterinary Science 88:Article 102938. https://doi.org/10.1016/j.jevs.2020.102938 2020
- Thompson, Riley E., Brown, Megan E., Helmick, Kelly, Whitlock, Brian K., and Pukazhenthi, Budhan S. 2020. "Persian onager (Equus hemionus onager) endometrial explant cryopreservation and in vitro culture." Animal Reproduction Science 217:Article 106459. https://doi.org/10.1016/j.anireprosci.2020.106459 2020
- Wildt, David, Miller, Philip, Koepfli, Klaus-Peter, Pukazhenthi, Budhan, Palfrey, Katy, Livingston, Gavin, Beetem, Dan, Shurter, Stephen, Gregory, Jimmy, Takács, Michael, and Snodgrass, Kelley. 2019. "Breeding Centers, Private Ranches, and Genomics for Creating Sustainable Wildlife Populations." Bioscience 69 (11):928-943. https://doi.org/10.1093/biosci/biz091 2019
- Thompson, Riley E., Johnson, Aime K., Prado, Tulio M., Premanandan, Christopher, Brown, Megan E., Whitlock, Brian K., and Pukazhenthi, Budhan S. 2019. "Dimethyl sulfoxide maintains structure and function of cryopreserved equine endometrial explants." Cryobiology 91:90-96. https://doi.org/10.1016/j.cryobiol.2019.10.006 2019
- Singh, Ram Pratap, Escobar, Enrique, Wildt, David E., Patel, Seema, Costa, Guilherme M. J., and Pukazhenthi, Budhan. 2019. "Effect of sphingosine-1-phosphate on cryopreserved sheep testicular explants cultured in vitro." Theriogenology 128:184-192. https://doi.org/10.1016/j.theriogenology.2019.01.038 2019
- Gibson, Keylie M., Nguyen, Bryan N., Neumann, Laura M., Miller, Michele, Buss, Peter, Daniels, Savel, Ahn, Michelle J., Crandall, Keith A., and Pukazhenthi, Budhan. 2019. "Gut microbiome differences between wild and captive black rhinoceros - implications for rhino health." Scientific Reports 9:7570. https://doi.org/10.1038/s41598-019-43875-3 2019
- Putman, Sarah B., Brown, Janine L., Saffoe, Craig, Franklin, Ashley D., and Pukazhenthi, Budhan S. 2019. "Linkage between fecal androgen and glucocorticoid metabolites, spermaturia, body weight and onset of puberty in male African lions (Panthera leo)." PloS One 14 (7):1-16. https://doi.org/10.1371/journal.pone.0217986 2019
- Koepfli, Klaus-Peter, Tamazian, Gaik, Wildt, David E., Dobrynin, Pavel, Kim, Changhoon, Frandsen, Paul B., Godinho, Raquel, Yurchenko, Andrey A., Komissarov, Aleksey, Krasheninnikova, Ksenia, Kliver, Sergei, Kolchanova, Sofia, Gonçalves, Margarida, Carneiro, Miguel, Vaz Pinto, Pedro, Ferrand, Nuno, Maldonado, Jesús E., Ferrie, Gina M., Chemnick, Leona, Ryder, Oliver A., Johnson, Warren E., Comizzoli, Pierre, O'Brien, Stephen J., and Pukazhenthi, Budhan S. 2019. "Whole Genome Sequencing and Re-sequencing of the Sable Antelope (Hippotragus niger): A Resource for Monitoring Diversity in ex Situ and in Situ Populations." G3 (Bethesda, Md.) 9 (6):1785-1793. https://doi.org/10.1534/g3.119.400084 2019
- Malo, Clara, Crichton, Elizabeth G., Morrell, Jane M., Pukazhenthi, Budhan S., Johannisson, Anders, Splan, Rebecca, and Skidmore, Julian A. 2018. "Colloid centrifugation of fresh semen improves post-thaw quality of cryopreserved dromedary camel spermatozoa." Animal Reproduction Science 192:28-34. https://doi.org/10.1016/j.anireprosci.2018.02.005 2018
- Brown, Megan E., Singh, Ram P., Pukazhenthi, Budhan, Keefer, Carol L., and Songsasen, Nucharin. 2018. "Cryopreservation effects on sperm function and fertility in two threatened crane species." Cryobiology 82:148-154. https://doi.org/10.1016/j.cryobiol.2018.01.010 2018
- Jacobs, R. D., Ealy, A. D., Pennington, P. M., Pukazhenthi, Budhan S., Warren, L. K., Wagner, A. L., Johnson, A. K., Hess, T. M., Knight, J. W., and Splan, R. K. 2018. "Dietary Supplementation of Algae-derived Omega-3 Fatty Acids Influences Endometrial and Conceptus Transcript Profiles in Mares." Journal of Equine Veterinary Science 62:66-75. https://doi.org/10.1016/j.jevs.2017.08.001 2018
- Wojtusik, Jessye, Wang, Yan, and Pukazhenthi, Budhan S. 2018. "Pretreatment with cholesterol-loaded cyclodextrins prevents loss of motility associated proteins during cryopreservation of addra gazelle (Nanger dama ruficollis) spermatozoa." Cryobiology 81:74-80. https://doi.org/10.1016/j.cryobiol.2018.02.007 2018
- Wojtusik, Jessye, Brown, Janine L., and Pukazhenthi, Budhan S. 2017. "Non-invasive hormonal characterization of the ovarian cycle, pregnancy, and seasonal anestrus of the female addra gazelle (Nanger dama ruficollis)." Theriogenology 95:96-104. https://doi.org/10.1016/j.theriogenology.2017.02.019 2017
- Vansandt, Lindsey M., Livesay, Janelle L., Dickson, Melissa Joy, Li, Lei, Pukazhenthi, Budhan S., and Keefer, Carol L. 2016. "Conservation of spermatogonial stem cell marker expression in undifferentiated felid spermatogonia." Theriogenology 86 (4):1022-1035. https://doi.org/10.1016/j.theriogenology.2016.03.031 2016
- Crichton, Elizabeth G., Malo, Clara, Pukazhenthi, Budhan S., Nagy, Peter, and Skidmore, Julian A. 2016. "Evaluation of cholesterol- treated dromedary camel sperm function by heterologous IVF and AI." Animal Reproduction Science 174:20-28. https://doi.org/10.1016/j.anireprosci.2016.08.013 2016
- Panyaboriban, Saritvich, Pukazhenthi, Budhan S., Brown, Megan E., Crowe, Chris, Lynch, Warren E., Singh, Ram P., Techakumphu, Mongkol, and Songsasen, Nucharin. 2016. "Influence of cooling and thawing conditions and cryoprotectant concentration on frozen-thawed survival of white-naped crane (Antigone vipio) spermatozoa." Cryobiology 73 (2):209-215. https://doi.org/10.1016/j.cryobiol.2016.07.009 2016
- Wojtusik, Jessye, Pennington, Parker, Songsasen, Nucharin, Padilla, Luis R., Citino, Scott B., and Pukazhenthi, Budhan S. 2016. "Pretreatment of Addra gazelle (Nanger dama ruficollis) spermatozoa with cholesterol-loaded cyclodextrins improves cryosurvival." Cryobiology 73 (3):388-395. https://doi.org/10.1016/j.cryobiol.2016.08.012 2016
- Panyaboriban, Saritvich, Singh, Ram Pratap, Songsasen, Nucharin, Padilla, Luis, Brown, Janine, Reed, Dolores, Techakumphu, Mongkol, and Pukazhenthi, Budhan S. 2016. "Reproductive seasonality and sperm cryopreservation in the male tufted deer (Elaphodus cephalophus)." Theriogenology 86 (4):914-923. https://doi.org/10.1016/j.theriogenology.2016.03.014 2016
- Malo, C., Morrell, J. M., Crichton, E. G., Pukazhenthi, Budhan S., and Skidmore, J. A. 2016. "Use of colloid single layer centrifugation for dromedary camel semen: Effect of initial dilution and comparison of two colloids on sperm quality parameters." Animal Reproduction Science 169:123. https://doi.org/10.1016/j.anireprosci.2016.03.068 2016
- Jacobs, R. D., Ealy, A. D., Pennington, P. M., Pukazhenthi, Budhan S., Warren, L. K., Wagner, A. L., Johnson, A. K., Hess, T. M., and Splan, R. K. 2015. "130 Effect of dietary omega-3 supplementation on equine endometrial and embryonic gene expression." Journal of Equine Veterinary Science 35 (5):438-439. https://doi.org/10.1016/j.jevs.2015.03.142 2015
- Burnik Šturm, Martina, Pukazhenthi, Budhan S., Reed, Dolores, Ganbaatar, Oyunsaikhan, Sušnik, Stane, Haymerle, Agnes, Voigt, Christian C., and Kaczensky, Petra. 2015. "A protocol to correct for intra- and interspecific variation in tail hair growth to align isotope signatures of segmentally cut tail hair to a common time line: Aligning isotope signatures of hair segments to the correct time line." Rapid Communications in Mass Spectrometry 29 (11):1047-1054. https://doi.org/10.1002/rcm.7196 2015
- Putman, Sarah B., Brown, Janine L., Franklin, Ashley D., Schneider, Emily C., Boisseau, Nicole P., Asa, Cheryl S., and Pukazhenthi, Budhan S. 2015. "Characterization of Ovarian Steroid Patterns in Female African Lions (Panthera leo), and the Effects of Contraception on Reproductive Function." PloS One 10 (10):https://doi.org/10.1371/journal.pone.0140373 2015
- Crichton, Elizabeth G., Pukazhenthi, Budhan S., Billah, M., and Skidmore, Julian A. 2015. "Cholesterol addition aids the cryopreservation of dromedary camel (Camelus dromedarius) spermatozoa." Theriogenology 83 (2):168-174. https://doi.org/10.1016/j.theriogenology.2014.09.005 2015
- Daigle, C. L., Brown, Janine L., Carlstead, K., Pukazhenthi, Budhan S., Freeman, E. W., and Snider, R. J. 2015. "Multi-institutional survey of social, management, husbandry and environmental factors for the SSP African lion Panthera leo population: examining the effects of a breeding moratorium in relation to reproductive success." International Zoo Yearbook 49 (1):198-213. https://doi.org/10.1111/izy.12073 2015
- Pukazhenthi, Budhan S., Nagashima, Jennifer B., Travis, Alexander J., Costa, Guilherme M., Escobar, Enrique N., França, Luiz R., and Wildt, David E. 2015. "Slow freezing, but not vitrification supports complete spermatogenesis in cryopreserved, neonatal sheep testicular xenografts." PloS One 10 (4):https://doi.org/10.1371/journal.pone.0123957 2015
- Pukazhenthi, Budhan S., Johnson, Aime, Guthrie, H. David, Songsasen, Nucharin, Padilla, Luis R., Wolfe, Barbara A., da Silva, Marco Coutinho, Alvarenga, Marco A., and Wildt, David E. 2014. "Improved Sperm Cryosurvival in Diluents Containing Amides versus Glycerol in the Przewalski's Horse (Equus ferus przewalskii)." Cryobiology 68 (2):205-214. https://doi.org/10.1016/j.cryobiol.2014.01.013 2014
- Pukazhenthi, Budhan S., Quse, Viviana, Hoyer, Mark, Gastelaars, Heleen Van Engeldorp, and Brown, Janine L. 2013. "A review of the reproductive biology and breeding management of tapirs (Tapirus sp.)." Integrative Zoology 8 (1 (special issue)):18-34. https://doi.org/10.1111/j.1749-4877.2012.12008.x 2013
- Schook, Mandi W., Wildt, David E., Weiss, Rachael B., Wolfe, Barbara A., Archibald, Kate E., and Pukazhenthi, Budhan S. 2013. "Fundamental Studies of the Reproductive Biology of the Endangered Persian Onager (Equus hemionus onager) Result in First Wild Equid Offspring from Artificial Insemination." Biology of reproduction 89 (2):41. https://doi.org/10.1095/biolreprod.113.110122 2013
- Kiso, Wendy K., Selvaraj, Vimal, Nagashima, Jennifer B., Asano, Atsushi, Brown, Janine L., Schmitt, Dennis L., Leszyk, John, Travis, Alexander J., and Pukazhenthi, Budhan S. 2013. "Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality." PLoS ONE 8 (8):e71033. https://doi.org/10.1371/journal.pone.0071033 2013
- Jewgenow, K., Pukazhenthi, Budhan S., and Schoen, J. 2012. "Analysis of Sertoli cell efficiency allows the differentiation between two fundamentally different forms of feline teratospermia." Theriogenology 79 (2):261-266. https://doi.org/10.1016/j.theriogenology.2012.08.014 2012
- Vick, Mandi,M., Wildt, David E., Turner, Jessica B., Palme, Rupert, Wolfe, Barbara A., and Pukazhenthi, Budhan S. 2012. "Glucocorticoid response to changes in enclosure size and human proximity in the Persian onager (Equus hemionus onager)." Stress 15 (1):52-61. https://doi.org/10.3109/10253890.2011.578267 2012
- Vansandt, L. M., Pukazhenthi, Budhan S., and Keefer, C. L. 2012. "Molecular Markers of Spermatogonial Stem Cells in the Domestic Cat." Reproduction in Domestic Animals 47 (Supplement S6):256-260. https://doi.org/10.1111/rda.12079 2012
- Stewart, Rosemary A., Pelican, Katharine M., Crosier, Adrienne E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, Mary Ann, and Howard, JoGayle. 2012. "Oral Progestin Priming Increases Ovarian Sensitivity to Gonadotropin Stimulation and Improves Luteal Function in the Cat." Biology of reproduction 87 (6):137. https://doi.org/10.1095/biolreprod.112.104190 2012
- Kiso, Wendy K., Asano, Atsushi, Travis, Alexander J., Schmitt, Dennis L., Brown, Janine L., and Pukazhenthi, Budhan S. 2012. "Pretreatment of Asian elephant (Elephas maximus) spermatozoa with cholesterol-loaded cyclodextrins and glycerol addition at 4°C improves cryosurvival." Reproduction, Fertility and Development 24 (8):1134-1142. https://doi.org/10.1071/RD11266 2012
- Pukazhenthi, Budhan S., Hagedorn, Mary M., Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2011. "Cryopreserving endangered species gametes, embryos and gonadal tissue: Challenges, successes and future directions." Cryobiology 63 (3):308-309. https://doi.org/10.1016/j.cryobiol.2011.09.014 2011
- Pukazhenthi, Budhan S., Togna, Gina Della, Padilla, Luis R., Smith, Diorene, Sanchez, Carlos R., Pelican, Katey, and Sanjur, Oris I. 2011. "Ejaculate Traits and Sperm Cryopreservation in the Endangered Baird's Tapir (Tapirus bairdii)." Journal of Andrology 32 (3):260-270. https://doi.org/10.2164/jandrol.110.011833 2011
- Kiso, Wendy K., Brown, Janine L., Siewerdt, Frank, Schmitt, Dennis L., Olson, Deborah, Crichton, Elizabeth G., and Pukazhenthi, Budhan S. 2011. "Liquid Semen Storage in Elephants (Elephas maximus and Loxodonta africana): Species Differences and Storage Optimization." Journal of andrology 32 (4):420-431. https://doi.org/10.2164/jandrol.110.011460 2011
- Comizzoli, Pierre, Pukazhenthi, Budhan S., and Wildt, David E. 2011. "The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model." Human Reproduction 26 (8):2165-2177. https://doi.org/10.1093/humrep/der176 2011
- Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E., and Pukazhenthi, Budhan S. 2010. "Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus)." Theriogenology 73 (1):112-119. https://doi.org/10.1016/j.theriogenology.2009.08.007 2010
- Pelican, K. M., Spindler, Rebecca E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, M. A., and Howard, JoGayle. 2010. "Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat." Biology of Reproduction 83 (4):558-567. https://doi.org/10.1095/biolreprod.109.083360 2010
- Songsasen, Nucharin, Fickes, A., Pukazhenthi, Budhan S., and Wildt, David E. 2009. "Follicular morphology, oocyte diameter and localization of fibroblast growth factors in the domestic dog ovary." Reproduction in Domestic Animals (Supplement 2) 44:65-70. 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes." Reproduction in Domestic Animals 44:269-274. 2009
- Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan S., and Songsasen, Nucharin. 2009. "Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health." Molecular reproduction and development 77 (5):397-409. https://doi.org/10.1002/mrd.21137 2009
- Schoen, J., Neumann, S., Wildt, David E., Pukazhenthi, Budhan S., and Jewgenow, K. 2009. "Localization of estrogen receptors in the epididymis during sexual maturation of the domestic cat." Reproduction in Domestic Animals 44:294-301. 2009
- Godard, N. M., Pukazhenthi, Budhan S., Wildt, David E., and Comizzoli, Pierre. 2009. "Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model." Fertility and sterility 91 (5):2051-2060. https://doi.org/10.1016/j.fertnstert.2008.05.069 2009
- Jewgenow, Katarina, Neubauer, Katrin, Blottner, Steffen, Schon, Jennifer, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "Reduced Germ Cell Apoptosis During Spermatogenesis in the Teratospermic Domestic Cat." Journal of andrology 30 (4):460-468. https://doi.org/10.2164/jandrol.108.006726 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2008. "Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat." Molecular reproduction and development 75:345-354. 2008
- Crosier, Adrienne E., Henghali, Josephine N., Howard, JoGayle, Pukazhenthi, Budhan S., Terrell, Kimberly A., Marker, Laurie L., and Wildt, David E. 2008. "Improved Quality of Cryopreserved Cheetah (Acinonyx jubatus) Spermatozoa after Centrifugation Through Accudenz." Journal of Andrology 30 (3):298. https://doi.org/10.2164/jandrol.108.006239 2008
- Baudi, D. L. K., Jewgenow, K., Pukazhenthi, Budhan S., Spercoski, K. M., Santos, A. S., Reghelin, A. L. S., Candido, M. V., Javorouski, M. L., Muller, G., and Morais, R. N. 2008. "Influence of cooling rate on the ability of frozen-thaw spermatozoa to bind to heterologous zona pellucida as assessed by competitive in vitro binding assays in the Ocelot (Leopardus pardalis) and Tigrina (Leopardus tigrinus)." Theriogenology 69:204-211. 2008
- Pukazhenthi, Budhan S., Santymire, Rachel, Crosier, Adrienne E., Howard, JoGayle, and Wildt, David E. 2007. "Challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival." Society for Reproduction and Fertility (Supplement) 65:433-446. 2007
- Kim, Y., Selvaraj, V., Pukazhenthi, Budhan S., and Travis, A. J. 2007. "Effect of donor age on success of spermatogenesis in feline testis xenografts." Reproduction, Fertility, and Development 19 (7):869-876. 2007
- Crosier, Adrienne E., Marker, Laurie, Howard, JoGayle, Pukazhenthi, Budhan S., Henghali, Josephine N., and Wildt, David E. 2007. "Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season, and captivity." Reproduction, Fertility, and Development 19:370-382. 2007
-
chapter
- Wildt, David E., Howard, JoGayle, Pelican, K., Brown, J., and Pukazhenthi, Budhan S. 2009. "Contributions of reproductive science to wild felid conservation." in Iberian Lynx Ex-situ Conservation: An interdisciplinary approach., edited by Breitenmoser, U., Fundación Biodiversidad. 2009
Other
Location
- National Zoological Park Academic Department
Smithsonian Conservation Commons Biography
- The primary goal of Budhan Pukazhenthi's research is the conservation of critically endangered species including ungulates and elephants in captivity and in the wild. His research interests include understanding the fundamental reproductive biology of rare and endangered species, mitigating infertility, developing assisted reproductive technologies (artificial insemination and in vitro fertilization/embryo transfer), and gamete and embryo cryopreservation. Recently his team produced the world's first Przewalski's horse foal via artificial insemination. He is also studying the reproductive biology of African lions in an effort to enhance reproduction in zoological institutions and developing innovative technologies for preservation of male and female gametes and reproductive tissues (testes and ovarian tissue cryopreservation).
