Wildt, David (1950-2020)
Fellowship Advisor Inactive National Zoological Park staffReproduction and endocrinology of wildlife species; small population breeding, management and conservation.
Background And Education
Education And Training
- B.A., Illinois State University
- M.S., Michigan State University
- Ph.D., Michigan State University
Awards And Honors
Public Biography
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UPDATE: David Wildt passed away on January 15, 2020. His obituary is available here.
David Wildt was a senior scientist emeritus and formerly led the Center for Species Survival team that generates much of what we now know about how many wildlife species reproduce. He also managed the wildlife animal collection at the Smithsonian Conservation and Biology Institute's 3,200 acre facility near Front Royal, Virginia. He used basic and applied research to create new knowledge that lead to better management and conservation of small populations, especially endangered species. While centered on reproduction, his research efforts connected to genetics, veterinary medicine, behavior, nutrition, ecology and the reintroduction of species to nature. This cross-disciplinary integration through partnerships was fundamental to Wildt's philosophy.
Wildt described the impact of small populations and inbreeding on sperm form and function, including on free-living cheetahs and lions. He and his team described the biological processes related to reproductive cyclicity, sperm, egg and embryo development in carnivores, especially felids and canids. This information has been used to produce young by assisted breeding technologies. Wildt's laboratory was the first to produce domestic cat kittens by fertilization, later applied successfully to the tiger. His team conducted the first successful transcontinental shipment of frozen sperm from a threatened species (a wild cheetah in Namibia) to produce a cub by artificial insemination in North America. His laboratory also produced black-footed ferret kits using sperm that had been cryo-banked for 20 years ' a milestone that increases gene diversity in the recovering population of this critically endangered species.
Wildt focused on increasing the speed and scale of applying animal expertise and reproductive knowledge to creating sustainable populations through multi-disciplinary/institutional partnerships. For example, his extensive studies in China that increased knowledge about giant pandas; when applied, lessons learned helped more than triple giant panda numbers in the global ex situ collection over the last 15 years. Another example is the creation of the Conservation Centers for Species Survival (C2S2), large-sized breeding facilities. These facilities cooperate and share resources to reproduce animals beyond a traditional urban zoo model. There, breeding programs for cheetahs and African antelopes are being coordinated on a large-scale in spacious pastures with natural group sizes and minimal public interference. Such partnerships, including those with private sector ranchers, increase the number of animals in insurance populations and available for scientific discovery, all while striving for self-sustainability.
Wildt received scientific achievement awards from American Association of Zoo Veterinarians, the Association of Zoos and Aquariums and Illinois State University. He also delivered keynote lectures at prestigious conferences including the International Embryo Transfer Society, European Society of Human Reproduction and Embryology, and American Society of Reproductive Medicine. In 2017, Wildt received the Smithsonian Distinguished Scholar Award in the Sciences. The award, first given in 2000, celebrates excellence in all branches of Smithsonian scholarship by honoring the sustained achievement of two outstanding Smithsonian scholars each year ' one in the sciences and one in the humanities.
Smithsonian Conservation Commons Biography
- David Wildt leads the Center for Species Survival team that has generated much of what we now know about how many wildlife species reproduce (from frogs to elephants). He also manages the wildlife animal collection at SCBI's 3,200 acre facility near Front Royal, VA. Basic and applied research is devoted to creating new knowledge that leads to better management and conservation of small populations, especially of endangered species. While centered on reproduction, research efforts are connected to genetics, veterinary medicine, behavior, nutrition, ecology and the reintroduction of species to nature. This cross-disciplinary integration through partnerships is fundamental to Wildt's philosophy.
Research And Grants
Co-principal Investigator On
Investigator On
Publications
Selected Publications
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Article
- Kliver, Sergei, Houck, Marlys L., Perelman, Polina L., Totikov, Azamat, Tomarovsky, Andrei, Dudchenko, Olga, Omer, Arina D., Colaric, Zane, Weisz, David, Aiden, Erez Lieberman, Chan, Saki, Hastie, Alex, Komissarov, Aleksey, Ryder, Oliver A., Graphodatsky, Alexander, Johnson, Warren E., Maldonado, Jesús E., Pukazhenthi, Budhan S., Marinari, Paul E., Wildt, David E., and Koepfli, Klaus-Peter. 2023. "Chromosome-length genome assembly and karyotype of the endangered black-footed ferret (Mustela nigripes)." Journal of Heredity, https://doi.org/10.1093/jhered/esad035. 2023
- Brown, Megan E., Pukazhenthi, Budhan, Olsen, Glenn H., Crowe, Chris, Lynch, Warren, Songsasen, Nucharin, and Wildt, David E. 2023. "Low estradiol production of non-laying whooping cranes (Grus americana) is associated with the failure of small follicles to enter follicular hierarchy." General and comparative endocrinology, 338. https://doi.org/10.1016/j.ygcen.2023.114280. 2023
- Humble, Emily, Dobrynin, Pavel, Senn, Helen, Chuven, Justin, Scott, Alan F., Mohr, David W., Dudchenko, Olga, Omer, Arina D., Colaric, Zane, Lieberman Aiden, Erez, Al Dhaheri, Shaikha Salem, Wildt, David, Oliaji, Shireen, Tamazian, Gaik, Pukazhenthi, Budhan, Ogden, Rob, and Koepfli, Klaus-Peter. 2020. "Chromosomal-level genome assembly of the scimitar-horned oryx: Insights into diversity and demography of a species extinct in the wild." Molecular Ecology Resources, https://doi.org/10.1111/1755-0998.13181. 2020
- Gooley, Rebecca M., Tamazian, Gaik, Castaneda-Rico, Susette, Murphy, Katherine R., Dobrynin, Pavel, Ferrie, Gina M., Haefele, Holly, Maldonado, Jesus E., Wildt, David E., Pukazhenthi, Budhan S., Edwards, Cody W., and Koepfli, Klaus-Peter. 2020. "Comparison of genomic diversity and structure of sable antelope (Hippotragus niger) in zoos, conservation centers, and private ranches in North America." Evolutionary Applications, 13, (8) 2143–2154. https://doi.org/10.1111/eva.12976. 2020
- Nagashima, Jennifer B., Wildt, David E., Travis, Alexander J., and Songsasen, Nucharin. 2019. "Activin promotes growth and antral cavity expansion in the dog ovarian follicle." Theriogenology, 129 168–177. https://doi.org/10.1016/j.theriogenology.2019.02.018. 2019
- Wildt, David, Miller, Philip, Koepfli, Klaus-Peter, Pukazhenthi, Budhan, Palfrey, Katy, Livingston, Gavin, Beetem, Dan, Shurter, Stephen, Gregory, Jimmy, Takács, Michael, and Snodgrass, Kelley. 2019. "Breeding Centers, Private Ranches, and Genomics for Creating Sustainable Wildlife Populations." Bioscience, 69, (11) 928–943. https://doi.org/10.1093/biosci/biz091. 2019
- Zhou, Ran, Wildt, David E., Keefer, Carol L., and Comizzoli, Pierre. 2019. "Combinations of Growth Factors Regulating LIF/STAT3, WNT, and FGF2 Pathways Sustain Pluripotency-Related Proteins in Cat Embryonic Cells." Stem Cells and Development, 28, (5). https://doi.org/10.1089/scd.2018.0109. 2019
- Singh, Ram Pratap, Escobar, Enrique, Wildt, David E., Patel, Seema, Costa, Guilherme M. J., and Pukazhenthi, Budhan. 2019. "Effect of sphingosine-1-phosphate on cryopreserved sheep testicular explants cultured in vitro." Theriogenology, 128 184–192. https://doi.org/10.1016/j.theriogenology.2019.01.038. 2019
- Santymire, R. M., Lonsdorf, E., V., Lynch, C. M., Wildt, D. E., Marinari, P. E., Kreeger, S., and Howard, J. G. 2019. "Inbreeding causes decreased seminal quality affecting pregnancy and litter size in the endangered black-footed ferret." Animal Conservation, 22, (4) 331–340. https://doi.org/10.1111/acv.12466. 2019
- Koepfli, Klaus-Peter, Tamazian, Gaik, Wildt, David E., Dobrynin, Pavel, Kim, Changhoon, Frandsen, Paul B., Godinho, Raquel, Yurchenko, Andrey A., Komissarov, Aleksey, Krasheninnikova, Ksenia, Kliver, Sergei, Kolchanova, Sofia, Gonçalves, Margarida, Carneiro, Miguel, Vaz Pinto, Pedro, Ferrand, Nuno, Maldonado, Jesús E., Ferrie, Gina M., Chemnick, Leona, Ryder, Oliver A., Johnson, Warren E., Comizzoli, Pierre, O'Brien, Stephen J., and Pukazhenthi, Budhan S. 2019. "Whole Genome Sequencing and Re-sequencing of the Sable Antelope (Hippotragus niger): A Resource for Monitoring Diversity in ex Situ and in Situ Populations." G3 (Bethesda, Md.), 9, (6) 1785–1793. https://doi.org/10.1534/g3.119.400084. 2019
- Thongkittidilok, Chommanart, Singh, Ram Pratap, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2018. "Insulin promotes preantral follicle growth and antrum formation through temporal expression of genes regulating steroidogenesis and water transport in the cat." Reproduction Fertility and Development, 30, (10) 1369–1379. https://doi.org/10.1071/RD17454. 2018
- Lee, P. C., Wildt, David E., and Comizzoli, Pierre. 2018. "Proteomic analysis of germinal vesicles in the domestic cat model reveals candidate nuclear proteins involved in oocyte competence acquisition." Molecular Human Reproduction, 24, (1) 14–26. https://doi.org/10.1093/molehr/gax059. 2018
- Fujihara, Mayako, Yamamizu, Kohei, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2018. "Retinoic acid promotes in vitro follicle activation in the cat ovary by regulating expression of matrix metalloproteinase 9." Plos One, 13, (8) 1–14. https://doi.org/10.1371/journal.pone.0202759. 2018
- Crosier, Adrienne E., Comizzoli, Pierre, Koester, Diana C., and Wildt, David E. 2017. "Circumventing the natural, frequent oestrogen waves of the female cheetah (Acinonyx jubatus) using oral progestin (Altrenogest)." Reproduction Fertility and Development, 29, (8) 1486–1498. https://doi.org/10.1071/RD16007. 2017
- Koester, Diana C., Freeman, Elizabeth W., Wildt, David E., Terrell, Kimberly A., Franklin, Ashley D., Meeks, Karen, and Crosier, Adrienne E. 2017. "Group management influences reproductive function of the male cheetah (Acinonyx jubatus)." Reproduction, fertility, and development, 29, (3) 496–508. https://doi.org/10.1071/RD15138. 2017
- Koester, Diana C., Wildt, David E., Maly, Morgan, Comizzoli, Pierre, and Crosier, Adrienne E. 2017. "Non-invasive identification of protein biomarkers for early pregnancy diagnosis in the cheetah (Acinonyx jubatus)." PloS One, 12, (12) 1–21. https://doi.org/10.1371/journal.pone.0188575. 2017
- Koester, Diana C., Wildt, David E., Brown, Janine L., Meeks, Karen, and Crosier, Adrienne E. 2017. "Public Exposure and Number of Conspecifics have no Influence on Ovarian and Adrenal Activity in the Cheetah (Acinonyx jubatus)." General and comparative endocrinology, 243 120–129. https://doi.org/10.1016/j.ygcen.2016.11.010. 2017
- Thongkittidilok, Chommanart, Wildt, David E., and Songsasen, Nucharin. 2017. "Responsiveness of intraovarian dog follicles in vitro to epidermal growth factor and vascular endothelial growth factor depends on ovarian donor age." Reproduction in Domestic Animals, 52, (Supplement S2) 114–122. https://doi.org/10.1111/rda.12852. 2017
- Songsasen, Nucharin, Thongkittidilok, Chommanart, Yamamizu, K., Wildt, David E., and Comizzoli, Pierre. 2017. "Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model." Theriogenology, 90 228–236. https://doi.org/10.1016/j.theriogenology.2016.12.006. 2017
- Thuwanut, Paweena, Comizzoli, Pierre, Wildt, David E., Keefer, Carol L., and Songsasen, Nucharin. 2017. "Stem cell factor promotes in vitro ovarian follicle development in the domestic cat by upregulating c-kit mRNA expression and stimulating the phosphatidylinositol 3-kinase/AKT pathway." Reproduction Fertility and Development, 29, (7) 1356–1368. https://doi.org/10.1071/RD16071. 2017
- Terrell, Kimberly A., Crosier, Adrienne E., Wildt, David E., O'Brien, Stephen J., Anthony, Nicola M., Marker, Laurie, and Johnson, Warren E. 2016. "Continued decline in genetic diversity among wild cheetahs (Acinonyx jubatus) without further loss of semen quality." Biological Conservation, 200 192–199. https://doi.org/10.1016/j.biocon.2016.05.034. 2016
- Fujihara, M., Yamamizu, K., Wildt, David E., and Songsasen, Nucharin. 2016. "Expression pattern of matrix metalloproteinases changes during folliculogenesis in the cat ovary." Reproduction in Domestic Animals, 51, (5) 717–725. https://doi.org/10.1111/rda.12736. 2016
- Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2016. "Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis." Journal of Assisted Reproduction and Genetics, 33, (6) 783–794. https://doi.org/10.1007/s10815-016-0706-4. 2016
- Howard, J. G., Lynch, C., Santymire, Rachel M., Marinari, Paul E., and Wildt, David E. 2016. "Recovery of gene diversity using long-term cryopreserved spermatozoa and artificial insemination in the endangered black-footed ferret." Animal Conservation, 19, (2) 102–111. https://doi.org/10.1111/acv.12229. 2016
- Wildt, D. E., Lynch, C., Santymire, R. M., and Marinari, P. E. 2016. "Recovery of gene diversity using long-term cryopreserved spermatozoa and artificial insemination in the endangered black-footed ferret: response to commentaries." Animal Conservation, 19, (2) 118–119. https://doi.org/10.1111/acv.12277. 2016
- Nájera, Fernando, Brown, Janine L., Wildt, David E., Virolle, Laurie, Kongprom, Urarikha, Revuelta, Luis, and Goodrowe-Beck, Karen. 2015. "Body mass dynamics in hand reared clouded leopard (Neofelis nebulosa) cubs from birth to weaning." Zoo biology, 34, (3) 239–243. https://doi.org/10.1002/zoo.21206. 2015
- Nagashima, Jennifer B., Wildt, David E., Travis, Alexander J., and Songsasen, Nucharin. 2015. "Follicular size and stage and gonadotropin concentration affect alginate-encapsulated in vitro growth and survival of pre- and early antral dog follicles." Reproduction, Fertility and Development, 29, (2) 262–273. https://doi.org/10.1071/RD15004. 2015
- Dobrynin, Pavel, Liu, Shiping, Tamazian, Gaik, Xiong, Zijun, Yurchenko, Andrey A., Krasheninnikova, Ksenia, Kliver, Sergey, Schmidt-Küntzel, Anne, Koepfli, Klaus-Peter, Johnson, Warren E., Kuderna, Lukas F. K., García-Pérez, Raquel, Manuel, Marc de, Godinez, Ricardo, Komissarov, Aleksey, Makunin, Alexey, Brukhin, Vladimir, Qiu, Weilin, Zhou, Long, Li, Fang, Yi, Jian, Driscoll, Carlos, Antunes, Agostinho, Oleksyk, Taras K., Eizirik, Eduardo et al. 2015. "Genomic legacy of the African cheetah, Acinonyx jubatus." Genome biology 16:277. https://doi.org/10.1186/s13059-015-0837-4 2015
- Increased inflammation and decreased insulin sensitivity indicate metabolic disturbances in zoo-managed compared to free-ranging black rhinoceros (Diceros bicornis). General and Comparative Endocrinology. 217/218:10-19. 2015
- Koester, Diana C., Freeman, Elizabeth W., Brown, Janine L., Wildt, David E., Terrell, Kimberly A., Franklin, Ashley D., and Crosier, Adrienne E. 2015. "Motile Sperm Output by Male Cheetahs (Acinonyx jubatus) Managed Ex Situ Is Influenced by Public Exposure and Number of Care-Givers." PloS One, 10, (9) 1–21. https://doi.org/10.1371/journal.pone.0135847. 2015
- Lee, Pei-Chih, Wildt, David E., and Comizzoli, Pierre. 2015. "Nucleolar Translocation of Histone Deacetylase 2 Is Involved in Regulation of Transcriptional Silencing in the Cat Germinal Vesicle." Biology of reproduction, 93, (2) 33. https://doi.org/10.1095/biolreprod.115.129106. 2015
- Stewart, Rosemary A., Crosier, Adrienne E., Pelican, Katharine M., Pukazhenthi, Budhan S., Sitzmann, Brandon D., Porter, Tom E., Wildt, David E., Ottinger, Mary Ann, and Howard, JoGayle. 2015. "Progestin priming before gonadotrophin stimulation and AI improves embryo development and normalises luteal function in the cat." Reproduction Fertility and Development, 27, (2) 360–371. https://doi.org/10.1071/RD13274. 2015
- Pukazhenthi, Budhan S., Nagashima, Jennifer B., Travis, Alexander J., Costa, Guilherme M., Escobar, Enrique N., França, Luiz R., and Wildt, David E. 2015. "Slow freezing, but not vitrification supports complete spermatogenesis in cryopreserved, neonatal sheep testicular xenografts." PloS One, 10, (4). https://doi.org/10.1371/journal.pone.0123957. 2015
- Brown, M. E., Converse, S. J., Chandler, J. N., Crosier, A. L., Lynch, Warren E., Wildt, David E., Keefer, C. L., and Songsasen, Nucharin. 2015. "Time within reproductive season, but not age or inbreeding coefficient, affects seminal and sperm quality in the whooping crane (Grus americana)." Reproduction Fertility and Development, 29, (2) 294–306. https://doi.org/10.1071/RD15251. 2015
- Fujihara, Mayako, Comizzoli, Pierre, Keefer, Carol L., Wildt, David E., and Songsasen, Nucharin. 2014. "Epidermal Growth Factor (EGF) Sustains In Vitro Primordial Follicle Viability by Enhancing Stromal Cell Proliferation via MAPK and PI3K Pathways in the Prepubertal, but Not Adult Cat Ovary." Biology of Reproduction, 90, (4). https://doi.org/10.1095/biolreprod.113.115089. 2014
- Pukazhenthi, Budhan S., Johnson, Aime, Guthrie, H. David, Songsasen, Nucharin, Padilla, Luis R., Wolfe, Barbara A., da Silva, Marco Coutinho, Alvarenga, Marco A., and Wildt, David E. 2014. "Improved Sperm Cryosurvival in Diluents Containing Amides versus Glycerol in the Przewalski's Horse (Equus ferus przewalskii)." Cryobiology, 68, (2) 205–214. https://doi.org/10.1016/j.cryobiol.2014.01.013. 2014
- Comizzoli, Pierre and Wildt, David E. 2014. "Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options." Reproduction, Fertility and Development, 26, (1) 91–98. https://doi.org/10.1071/RD13259. 2014
- Johnson, Amy E. M., Freeman, Elizabeth W., Wildt, David E., and Songsasen, Nucharin. 2014. "Spermatozoa from the maned wolf (Chrysocyon brachyurus) display typical canid hyper-sensitivity to osmotic and freezing-induced injury, but respond favorably to dimethyl sulfoxide." Cryobiology, 68, (3) 361–370. https://doi.org/10.1016/j.cryobiol.2014.04.004. 2014
- Nájera, Fernando, Brown, Janine L., Kaufman, Krystle, Schwartz, Rick, Goodrowe, Karen, Asaithanmakul, Wisit, Aitken-Palmer, Copper, Kongprom, Urarikha, Wildt, David E., and Bush, Mitchell. 2014. "Swimmer Syndrome in a Clouded Leopard (Neofelis Nebulosa) Cub." Journal of Zoo and Wildlife Medicine, 45, (2) 386–388. https://doi.org/10.1638/2012-0289R2.1. 2014
- Schook, Mandi W., Wildt, David E., Weiss, Rachael B., Wolfe, Barbara A., Archibald, Kate E., and Pukazhenthi, Budhan S. 2013. "Fundamental Studies of the Reproductive Biology of the Endangered Persian Onager (Equus hemionus onager) Result in First Wild Equid Offspring from Artificial Insemination." Biology of reproduction, 89, (2) 41. https://doi.org/10.1095/biolreprod.113.110122. 2013
- Graves-Herring, Jennifer, Wildt, David E., and Comizzoli, Pierre. 2013. "Retention of Structure and Function of the Cat Germinal Vesicle after Air-Drying and Storage at Supra-Zero Temperature." Biology of Reproduction, 88, (6) 1–7. https://doi.org/10.1095/biolreprod.113.108472. 2013
- Hobbs, Rebecca Jane, Comizzoli, Pierre, Howard, JoGayle, and Wildt, David E. 2012. "Absence of Seasonal Changes in FSHR Gene Expression in the Cat Cumulus-Oocyte Complex in Vivo and in Vitro." Reproduction, 144 111–122. https://doi.org/10.1530/REP-12-0098. 2012
- Spercoski, Katherinne M., Morais , Rosana N., Morato, Ronaldo G., De Paula, Rogério C., Azevedo, Fernanda C., May-Júnior, Joares A., Santos, Jean P., Reghelin, Angela L., Wildt, David E., and Songsasen, Nucharin. 2012. "Adrenal activity in maned wolves is higher on farmlands and park boundaries than within protected areas." General and comparative endocrinology, 179, (2) 232–240. https://doi.org/10.1016/j.ygcen.2012.08.002. 2012
- Fujihara, Mayako, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2012. "Cat and Dog Primordial Follicles Enclosed in Ovarian Cortex Sustain Viability after In vitro Culture on Agarose Gel in a Protein-Free Medium." Reproduction in Domestic Animals, 47, (Supplement S6) 102–108. https://doi.org/10.1111/rda.12022. 2012
- Comizzoli, Pierre, Songsasen, Nucharin, Hagedorn, Mary M., and Wildt, David E. 2012. "Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species." Theriogenology, 78, (8) 1666–1681. https://doi.org/10.1016/j.theriogenology.2012.04.008. 2012
- Comparative cryopreservation of avian spermatozoa: effects of freezing and thawing rates on turkey and sandhill crane sperm cryosurvival. Animal Reproduction Science. 131:1-8. 2012
- Terrell, Kimberly A., Wildt, David E., Anthony, Nicola M., Bavister, Barry D., Leibo, S. P., Penfold, Linda M., Marker, Laurie L., and Crosier, Adrienne E. 2012. "Different patterns of metabolic cryo-damage in domestic cat (Felis catus) and cheetah (Acinonyx jubatus) spermatozoa." Cryobiology, 64, (2) 110–117. https://doi.org/10.1016/j.cryobiol.2011.12.006. 2012
- Vick, Mandi,M., Wildt, David E., Turner, Jessica B., Palme, Rupert, Wolfe, Barbara A., and Pukazhenthi, Budhan S. 2012. "Glucocorticoid response to changes in enclosure size and human proximity in the Persian onager (Equus hemionus onager)." Stress, 15, (1) 52–61. https://doi.org/10.3109/10253890.2011.578267. 2012
- Becker, Penny A., Miller, Philip S., Gunther, Micaela Szykman, Somers, Michael J., Wildt, David E., and Maldonado, Jesús E. 2012. "Inbreeding Avoidance Influences the Viability of Reintroduced Populations of African Wild Dogs (Lycaon pictus)." PLoS ONE, 7, (5) 1–11. https://doi.org/10.1371/journal.pone.0037181. 2012
- Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2012. "Increase in Histone Methylation in the Cat Germinal Vesicle Related to Acquisition of Meiotic and Developmental Competence." Reproduction in Domestic Animals, 47, (Supplement S6) 210–214. https://doi.org/10.1111/rda.12052. 2012
- Comizzoli, Pierre and Wildt, David E. 2012. "On the Horizon for Fertility Preservation in Domestic and Wild Carnivores." Reproduction in Domestic Animals, 47, (Supplement S6) 261–265. https://doi.org/10.1111/rda.12010. 2012
- Stewart, Rosemary A., Pelican, Katharine M., Crosier, Adrienne E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, Mary Ann, and Howard, JoGayle. 2012. "Oral Progestin Priming Increases Ovarian Sensitivity to Gonadotropin Stimulation and Improves Luteal Function in the Cat." Biology of reproduction, 87, (6) 137. https://doi.org/10.1095/biolreprod.112.104190. 2012
- Hagedorn, Mary M., Carter, Virginia L., Martorana, Kelly, Paresa, Malia K., Acker, Jason, Baums, Iliana B., Borneman, Eric, Brittsan, Michael, Byers, Michael, Henley, Michael, Laterveer, Michael, Leong, Jo-Ann, McCarthy, Megan, Meyers, Stuart, Nelson, Brian D., Petersen, Dirk, Tiersch, Terrence, Uribe, Rafael Cuevas, Woods, Erik, and Wildt, David E. 2012. "Preserving and Using Germplasm and Dissociated Embryonic Cells for Conserving Caribbean and Pacific Coral." PLoS ONE, 7, (3) 1–13. https://doi.org/10.1371/journal.pone.0033354. 2012
- Protracted Reproductive Seasonality in the Male Giant Panda (Ailuropoda Melanoleuca) Reflected by Patterns in Androgen Profiles, Ejaculate Characteristics, and Selected Behaviors. Biology of reproduction. 86:195. 2012
- Relationship of the Estrogen Surge and Multiple Mates to Cub Paternity in the Giant Panda (Ailuropoda melanoleuca): Implications for Optimal Timing of Copulation or Artificial Insemination. Biology of reproduction. 87:1-7. 2012
- Songsasen, Nucharin, Comizzoli, Pierre, Nagashima, Jennifer B., Fujihara, Mayako, and Wildt, David E. 2012. "The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro." Reproduction in Domestic Animals, 47, (Supplement S6) 13–18. https://doi.org/10.1111/rda.12067. 2012
- Songsasen, Nucharin, Wesselowski, Sonya, Carpenter, James W., and Wildt, David E. 2012. "The ability to achieve meiotic maturation in the dog oocyte is linked to glycolysis and glutamine oxidation." Molecular reproduction and development, 79, (3) 186–196. https://doi.org/10.1002/mrd.22011. 2012
- Gratwicke, Brian, Lovejoy, Thomas E., and Wildt, David E. 2012. "Will Amphibians Croak under the Endangered Species Act?" Bioscience, 62, (2) 197–202. https://doi.org/10.1525/bio.2012.62.2.13. 2012
- Songsasen, Nucharin, Guzy, C., and Wildt, David E. 2011. "121 Alginate-Fibrin Gel Matrix Promotes in Vitro Growth of Dog Secondary Follicles." Reproduction, Fertility and Development, 24, (1) 173. https://doi.org/10.1071/RDv24n1Ab121. 2011
- Swaisgood, Ronald R., Wei, Fuwen, McShea, William J., Wildt, David E., Kouba, Andrew J., and Zhang, Zejun. 2011. "Can science save the giant panda (Ailuropoda melanoleuca)? Unifying science and policy in an adaptive management paradigm." Integrative Zoology, 6, (3) 290–296. https://doi.org/10.1111/j.1749-4877.2011.00250.x. 2011
- Comparative Cryopreservation of Avian Spermatozoa: Benefits of Non-Permeating Osmoprotectants and ATP on Turkey and Crane Sperm Cryosurvival. Animal Reproduction Science. 123:242-248. 2011
- Pukazhenthi, Budhan S., Hagedorn, Mary M., Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2011. "Cryopreserving endangered species gametes, embryos and gonadal tissue: Challenges, successes and future directions." Cryobiology, 63, (3) 308–309. https://doi.org/10.1016/j.cryobiol.2011.09.014. 2011
- Terrell, Kimberly A., Wildt, David E., Anthony, Nicola M., Bavister, Barry D., Leibo, Stanley P., Penfold, Linda M., Marker, Laurie L., and Crosier, Adrienne E. 2011. "Glycolytic Enzyme Activity Is Essential for Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus) Sperm Motility and Viability in a Sugar-Free Medium." Biology of reproduction, 84, (6) 1198–1206. https://doi.org/10.1095/biolreprod.110.090225. 2011
- Songsasen, Nucharin, Woodruff, Teresa K., and Wildt, David E. 2011. "In vitro growth and steroidogenesis of dog follicles as influenced by the physical and hormonal microenvironment." Reproduction, 142 113–122. https://doi.org/10.1530/REP-10-0442. 2011
- Spiering, Penny A., Szykman, Micaela, Somers, Michael J., Wildt, David E., Walters, Michelle, Wilson, Amy S., and Maldonado, Jesús E. 2011. "Inbreeding, heterozygosity and fitness in a reintroduced population of endangered African wild dogs (Lycaon pictus)." Conservation Genetics, 2011, (12) 410–412. https://doi.org/10.1007/s10592-010-0147-z. 2011
- Crosier, Adrienne E., Comizzoli, Pierre, Baker, Tomas, Davidson, Autumn, Munson, Linda, Howard, JoGayle, Marker, Laurie L., and Wildt, David E. 2011. "Increasing Age Influences Uterine Integrity, but Not Ovarian Function or Oocyte Quality in the Cheetah (Acinonyx jubatus)." Biology of reproduction, 85, (2) 243–253. https://doi.org/10.1095/biolreprod.110.089417. 2011
- Terrell, Kimberly A., Wildt, David E., Anthony, Nicola M., Bavister, Barry D., Leibo, Stanley P., Penfold, Linda M., Marker, Laurie L., and Crosier, Adrienne E. 2011. "Oxidative Phosphorylation Is Essential for Felid Sperm Function, but Is Substantially Lower in Cheetah (Acinonyx jubatus) Compared to Domestic Cat (Felis catus) Ejaculate." Biology of reproduction, 85, (3) 473–481. https://doi.org/10.1095/biolreprod.111.092106. 2011
- Santymire, Rachel M., Brown, Janine L., Stewart, Rosemary A., Santymire, Robb C., Wildt, David E., and Howard, JoGayle. 2011. "Reproductive gonadal steroidogenic activity in the fishing cat (Prionailurus viverrinus) assessed by fecal steroid analyses." Animal Reproduction Science, 128, (1-4) 60–72. https://doi.org/10.1016/j.anireprosci.2011.09.001. 2011
- Kersey, David C., Wildt, David E., Brown, Janine L., Snyder, Rebecca J., Huang, Yan, and Monfort, Steven L. 2011. "Rising fecal glucocorticoid concentrations track reproductive activity in the female giant panda (Ailuropoda melanoleuca)." General and comparative endocrinology, 173, (2) 364–370. https://doi.org/10.1016/j.ygcen.2011.06.013. 2011
- Comizzoli, Pierre, Pukazhenthi, Budhan S., and Wildt, David E. 2011. "The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model." Human Reproduction, 26, (8) 2165–2177. https://doi.org/10.1093/humrep/der176. 2011
- Kersey, David C., Wildt, David E., Brown, Janine L., Snyder, Rebecca J., Huang, Yan, and Monfort, Steven L. 2010. "Endocrine milieu of perioestrus in the giant panda (Ailuropoda melanoleuca), as determined by non-invasive hormone measures." Reproduction, Fertility, and Development, 22 901–912. 2010
- Endocrine patterns of the estrous cycle and pregnancy of wildebeest in the serengeti ecosystem. General and Comparative Endocrinology. 166:365-371. 2010
- Terrell, Kimberly A., Wildt, David E., Anthony, N., Bavister, B. D., Leibo, S. P., Penfold, Linda M., Marker, L. L., and Crosier, Adrienne E. 2010. "Evidence for Compromised Metabolic Function and Limited Glucose Uptake in Spermatozoa from the Teratospermic Domestic Cat (Felis catus) and Cheetah (Acinonyx jubatus)." Biology of Reproduction, 83, (5) 833–841. https://doi.org/10.1095/biolreprod.110.085639. 2010
- Johnson, Warren E., Onorato, David P., Roelke, Melody E., Land, E. Darrell, Cunningham, Mark, Belden, Robert C., McBride, Roy, Jansen, Deborah, Lotz, Mark, Shindle, David, Howard, JoGayle, Wildt, David E., Penfold, Linda M., Hostetler, Jeffrey A., Oli, Madan K., and O'Brien, Stephen J. 2010. "Genetic Restoration of the Florida Panther." Science 329 (5999):1641-1645. https://doi.org/10.1126/science.1192891 2010
- Giant panda conservation science: how far we have come. Biology Letters. 6:143-145. 2010
- Gratwicke, Brian, Evans, Matthew J., Jenkins, Peter T., Kusrini, Mirza D., Moore, Robin D., Sevin, Jennifer, and Wildt, David E. 2010. "Is the international frog legs trade a potential vector for deadly amphibian pathogens?" Frontiers in Ecology and the Environment, 8, (8) 438–442. https://doi.org/10.1890/090111. 2010
- Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E., and Pukazhenthi, Budhan S. 2010. "Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus)." Theriogenology, 73, (1) 112–119. https://doi.org/10.1016/j.theriogenology.2009.08.007. 2010
- Stewart, R. A., Pelican, K. M., Brown, Janine L., Wildt, David E., Ottinger, M. A., and Howard, JoGayle. 2010. "Oral progestin induces rapid, reversible suppression of ovarian activity in the cat." General and comparative endocrinology, 166, (2) 409–416. https://doi.org/10.1016/j.ygcen.2009.12.016. 2010
- Kersey, David C., Wildt, David E., Brown, Janine L., Huang, Yan, Snyder, Rebecca J., and Monfort, Steven L. 2010. "Parallel and seasonal changes in gonadal and adrenal hormones in male giant pandas (Ailuropoda melanoleuca)." Journal of Mammalogy, 91, (6) 1496–1507. https://doi.org/10.1644/09-MAMM-A-404.1. 2010
- Pelican, K. M., Spindler, Rebecca E., Pukazhenthi, Budhan S., Wildt, David E., Ottinger, M. A., and Howard, JoGayle. 2010. "Progestin exposure before gonadotropin stimulation improves embryo development after in vitro fertilization in the domestic cat." Biology of Reproduction, 83, (4) 558–567. https://doi.org/10.1095/biolreprod.109.083360. 2010
- Spiering, Penny A., Somers, Michael J., Maldonado, Jesús E., Wildt, David E., and Gunther, Micaela Szykman. 2010. "Reproductive sharing and proximate factors mediating cooperative breeding in the African wild dog (Lycaon pictus)." Behavioral Ecology and Sociobiology, 64, (4) 583–592. https://doi.org/10.1007/s00265-009-0875-6. 2010
- Kersey, David C., Wildt, David E., Brown, Janine L., Snyder, R. J., Huang, Y., and Monfort, Steven L. 2010. "Unique Biphasic Progestagen Profile in Parturient and Nonparturient Giant Pandas (Ailuropoda melanoleuca) as Determined by Faecal Hormone Monitoring." Reproduction, 140 183–193. 2010
- Comizzoli, Pierre, Crosier, Adrienne E., Songsasen, Nucharin, Szykman, Micaela, Howard, JoGayle, and Wildt, David E. 2009. "Advances in reproductive science for wild carnivore conservation." Reproduction in Domestic Animals, 44 47–52. 2009
- Approaches and efficacy of artificial insemination in felids and mustelids. Theriogenology. 71:130-148. 2009
- Songsasen, Nucharin, Fickes, A., Pukazhenthi, Budhan S., and Wildt, David E. 2009. "Follicular morphology, oocyte diameter and localization of fibroblast growth factors in the domestic dog ovary." Reproduction in Domestic Animals (Supplement 2), 44 65–70. 2009
- May-Júnior, J. A., Songsasen, Nucharin, Azevedo, F. C., Santos, J. P., Paula, R. C., Rodrigues, F. H. G., Rodden, Melissa D., Wildt, David E., and Morato, R. G. 2009. "Hematology and blood chemistry status differs in free-ranging maned wolves (Chrysocyon brachyurus) living in the Serra da Canastra National Park (Brazil) versus adjacent farmlands." Journal of Zoo and Wildlife Diseases, 45 81–90. 2009
- Implementing artificial insemination as an effective tool for ex situ conservation of endangered avian species. Theriogenology. 71:200-213. 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes." Reproduction in Domestic Animals, 44 269–274. 2009
- Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan S., and Songsasen, Nucharin. 2009. "Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health." Molecular reproduction and development, 77, (5) 397–409. https://doi.org/10.1002/mrd.21137. 2009
- Schoen, J., Neumann, S., Wildt, David E., Pukazhenthi, Budhan S., and Jewgenow, K. 2009. "Localization of estrogen receptors in the epididymis during sexual maturation of the domestic cat." Reproduction in Domestic Animals, 44 294–301. 2009
- Godard, N. M., Pukazhenthi, Budhan S., Wildt, David E., and Comizzoli, Pierre. 2009. "Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model." Fertility and sterility, 91, (5) 2051–2060. https://doi.org/10.1016/j.fertnstert.2008.05.069. 2009
- Jewgenow, Katarina, Neubauer, Katrin, Blottner, Steffen, Schon, Jennifer, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "Reduced Germ Cell Apoptosis During Spermatogenesis in the Teratospermic Domestic Cat." Journal of andrology, 30, (4) 460–468. https://doi.org/10.2164/jandrol.108.006726. 2009
- Spiering, Penny A., Gunther, Micaela Szykman, Wildt, David E., Somers, Michael J., and Maldonado, Jesús E. 2009. "Sampling error in non-invasive genetic analyses of an endangered social carnivore." Conservation Genetics, 10 2005–2007. 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2008. "Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat." Molecular reproduction and development, 75 345–354. 2008
- Crosier, Adrienne E., Henghali, Josephine N., Howard, JoGayle, Pukazhenthi, Budhan S., Terrell, Kimberly A., Marker, Laurie L., and Wildt, David E. 2008. "Improved Quality of Cryopreserved Cheetah (Acinonyx jubatus) Spermatozoa after Centrifugation Through Accudenz." Journal of Andrology, 30, (3) 298. https://doi.org/10.2164/jandrol.108.006239. 2008
- Osmotic tolerance of avian spermatozoa: Influence of time, temperature, cryoprotectant and membrane ion pump function on sperm viability. Cryobiology. 56:8-14. 2008
- Priming with progestin, but not GnRH antagonist, induces a consistent endocrine response to exogenous gonadotropins in induced and spontaneously ovulating cats. Domestic animal endocrinology. 34:160-175. 2008
- Pukazhenthi, Budhan S., Santymire, Rachel, Crosier, Adrienne E., Howard, JoGayle, and Wildt, David E. 2007. "Challenges in cryopreserving endangered mammal spermatozoa: morphology and the value of acrosomal integrity as markers of cryo-survival." Society for Reproduction and Fertility (Supplement), 65 433–446. 2007
- Crosier, Adrienne E., Marker, Laurie, Howard, JoGayle, Pukazhenthi, Budhan S., Henghali, Josephine N., and Wildt, David E. 2007. "Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season, and captivity." Reproduction, Fertility, and Development, 19 370–382. 2007
- Fecal steroid monitoring for assessing gonadal and adrenal activity in the golden eagle and peregrine falcon. Journal of Comparative Physiology B. 177:609-622. 2007
- Songsasen, Nucharin, Spindler, Rebecca E., and Wildt, David E. 2007. "Requirement for, and patterns of, pyruvate and glutamine metabolism in the domestic dog oocyte in vitro." Molecular reproduction and development, 74 870–877. 2007
- Loeffler, I. Kati, Howard, JoGayle, Montali, Richard J., Hayek, Lee-Ann C., Dubovi, Edward, Zhang, Zhihe, Yan, Qigui, Guo, Wanzhu, and Wildt, David E. 2007. "Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications for species conservation." Journal of Zoo and Wildlife Medicine, 38, (4) 559–566. https://doi.org/10.1638/2006-0008R.1. 2007
- Santymire, R. M., Marinari, Paul E., Kreeger, J. S., Wildt, David E., and Howard, JoGayle. 2007. "Slow cooling prevents cold-induced damage to sperm motility and acrosomal integrity in the black-footed ferret (Mustela nigripes)." Reproduction, Fertility, and Development, 19 652–663. 2007
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Chapter
- Comizzoli, Pierre and Wildt, David E. 2012. "Centrosomal Functions and Dysfunctions in Cat Spermatozoa." In The Centrosome. Schatten, Heide, editor. 49–58. Humana Press. 2012
- Wildt, David E., Swanson, William F., Brown, Janine L., Sliwa, Alexander, and Vargas, Astrid. 2010. "Felids ex situ: managed programmes, research, and species recovery." In Biology and Conservation of Wild Felids. Macdonald, David W. and Loveridge, Andrew J., editors. 217–235. England; Cambridge, MA: Oxford University Press; Harvard University Press. In Wildland and Woodlands: A Forest Vision for New England. 2010
- Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2010. "Protecting and Extending Fertility for Females of Wild and Endangered Mammals." In Oncofertility. 87–100. Springer US. In Cancer Treatment and Research (vol. 156). 2010
- Wildt, David E., Howard, JoGayle, Pelican, K., Brown, J., and Pukazhenthi, Budhan S. 2009. "Contributions of reproductive science to wild felid conservation." In Iberian Lynx Ex-situ Conservation: An interdisciplinary approach.. Breitenmoser, U., editor. Madrid, Spain: Fundación Biodiversidad. 2009
Contact
Location
- National Zoo & Conservation Biology Institute Academic Department