Dr. Pierre Comizzoli has worked as a veterinarian in French Guyana and in the African Sahelo-Saharan region. He also has a master's degree from the University of Paris VI and a doctoral degree from the University of Tours on reproductive biotechnologies in bovine and deer species. During his doctoral studies, he described an original effect of the paternal component on early embryo development in both species, and produced in vitro the first transferable embryos in red deer and Japanese sika deer. Dr. Comizzoli is a staff scientist at the National Zoo and Conservation Biology Institute since 2002. He develops new projects on gamete and gonadal tissue cryo-banking for rare and endangered species. His comparative research on fertility preservation in various wild and domestic animal species creates interesting bridges with human reproductive health and medicine. Besides conducting research on cutting-edge approaches in fertility preservation and assisted reproduction, Dr. Comizzoli is also in charge of conservation projects on wild carnivores and ungulates in Northern Africa and in Southeast Asia. Dr. Comizzoli leads the Pan-Smithsonian Cryo-Initiative (PSCI) which aims to improve the management and use of biomaterial repositories within the entire Smithsonian. Since 2014, Dr. Comizzoli also works as a Senior Program Officer at the Office of the Under Secretary for Science and Research to develop pan-institutional efforts in One Health as well as in sustainability.
Silva, Andreia M., Pereira, Ana G., Bezerra, Luana G. P., Jeronimo Moreira, Samara S., Pereira, Alexsandra F., Oliveira, Moacir F., Comizzoli, Pierre, and Silva, Alexandre R. 2022. "Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Dasyprocta leporina Linnaeus, 1758)." Animals, 12, (6). Basel; Switzerland: MDPI, https://doi.org/10.3390/ani12060738.
2022
Macente, Beatrice Ingrid, Fonseca-Alves, Carlos Eduardo, Magalhaes, Georgia Mode, Tavares, Mariana Riboli, Mansano, Cleber Fernando Menegasso, Mouttham, Lara, Apparicio, Maricy, Toniollo, Gilson Helio, and Comizzoli, Pierre. 2022. "Influence of Vitrification Device, Warming Protocol, and Subsequent In Vitro Culture on Structural Integrity of Testicular Fragments from Adult Domestic Cats." Biopreservation and Biobanking, New Rochelle; NY: Mary Ann Liebert, Incorporated. https://doi.org/10.1089/bio.2021.0092.
2022
Comizzoli, Pierre, Loi, Pasqualino, Patrizio, Pasquale, and Hubel, Allison. 2022. "Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?" Journal of assisted reproduction and genetics, New York; NY: Springer/Plenum Publishers. https://doi.org/10.1007/s10815-021-02392-x.
2022
Holt, William, V. and Comizzoli, Pierre. 2022. "Opportunities and Limitations for Reproductive Science in Species Conservation." Annual Review of Animal Biosciences, 10 491–511. https://doi.org/10.1146/annurev-animal-013120-030858.
2022
Pomeroy, Kimball O., Comizzoli, Pierre, Rushing, John S., Lersten, Ivy L., and Nel-Themaat, Liesl. 2022. "The ART of cryopreservation and its changing landscape." Fertility and sterility, 469–476. 117, (3), New York; NY: Elsevier Science Incorporated. https://doi.org/10.1016/j.fertnstert.2022.01.018.
2022
Comizzoli, Pierre. 2021. "Adding new ingredients to the recipe for successful embryo transfers." Journal of assisted reproduction and geneticshttps://doi.org/10.1007/s10815-021-02148-72021
Paulson, Erika Elinor and Comizzoli, Pierre. 2021. "Endometrial receptivity and embryo implantation in carnivores-commonalities and differences with other mammalian species." Biology of reproduction, 771–783. 104, (4), Cary; North Carolina: Oxford University Press Incorporated. https://doi.org/10.1093/biolre/ioab001.
2021
Rowlison, Tricia, Ottinger, Mary Ann, and Comizzoli, Pierre. 2021. "Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model." Journal of assisted reproduction and geneticshttps://doi.org/10.1007/s10815-021-02214-02021
da Silva, Andreia Maria, Pereira, Ana Gloria, Brasil, Andreza Vieira, Macedo, Lua Barbalho, Souza-Junior, Joao, Bezerra de Moura, Carlos Eduardo, Pereira, Alexsandra Fernandes, de Oliveira, Moacir Franco, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2021. "Influence of freezing techniques and glycerol-based cryoprotectant combinations on the survival of testicular tissues from adult collared peccaries." Theriogenology 167:111-119. https://doi.org/10.1016/j.theriogenology.2021.03.0132021
Chavez, Daniela R., Lee, Pei-Chih, and Comizzoli, Pierre. 2021. "Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1." Frontiers in Cell and Developmental Biology 9:https://doi.org/10.3389/fcell.2021.6700212021
Zainuddin, Zainal Zahari, Sipangkui, Symphorosa, Kelana, Mohd Farqhan, Chee, Yap Keng, Tarmizi, Mohamed Reza Mohamed, and Comizzoli, Pierre. 2021. "Repeated Evaluations of Testes and Semen Characteristics in Two Binturongs (Arctictis binturong)." Frontiers in Veterinary Science 8:https://doi.org/10.3389/fvets.2021.6585732021
Comizzoli, Pierre, Pagenkopp Lohan, Katrina M., Muletz-Wolz, Carly, Hassell, James, and Coyle, Brian J. 2021. "The Interconnected Health Initiative: A Smithsonian Framework to Extend One Health Research and Education." Frontiers in Veterinary Science 8:https://doi.org/10.3389/fvets.2021.6294102021
Rowlison, Tricia and Comizzoli, Pierre. 2021. "The Knowns and Unknowns about Epididymal Extracellular Vesicles in Different Animal Species." Advanced Biology, Weinheim; Germany: Wiley-V C H Verlag GMBH. https://doi.org/10.1002/adbi.202101066.
2021
Amelkina, Olga, da Silva, Andreia M., Silva, Alexandre R., and Comizzoli, Pierre. 2021. "Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation." Bmc Genomics, 22, (1), London; England: BMC. https://doi.org/10.1186/s12864-021-08099-8.
2021
Comizzoli, Pierre and Ottinger, Mary Ann. 2021. "Understanding Reproductive Aging in Wildlife to Improve Animal Conservation and Human Reproductive Health." Frontiers in Cell and Developmental Biology 9:https://doi.org/10.3389/fcell.2021.6804712021
Silva, Andréia Maria da, Pereira, Alexsandra Fernandes, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2020. "Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation." Biopreservation and Biobanking 18 (3):235-243. https://doi.org/10.1089/bio.2020.00102020
Comizzoli, Pierre. 2020. "Integrating fertility preservation and cryo-banking into the conservation of rare and endangered deer species." Animal Production Science 60 (10):1227-1232. https://doi.org/10.1071/AN186742020
Lima, David Baruc Cruvinel, da Silva, Lucia Daniel Machado, Marinari, Paul, and Comizzoli, Pierre. 2020. "Long-Term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Mustela nigripes)." Animals 10 (10):1865-1865. https://doi.org/10.3390/ani101018652020
Zhou, Ran, Comizzoli, Pierre, and Keefer, Carol L. 2019. "Endogenous pluripotent factor expression after reprogramming cat fetal fibroblasts using inducible transcription factors." Molecular reproduction and development 86 (11):1671-1681. https://doi.org/10.1002/mrd.232572019
Comizzoli, Pierre, Brown, Janine L., and Holt, William V. 2019. "From the Ivory Tower to Reality! Conclusions of the New Edition." in Reproductive Sciences in Animal Conservation, edited by Comizzoli, Pierre, Brown, Janine L., and Holt, William V., 2nd ed. 545-550. Springer International Publishing AG. https://doi.org/10.1007/978-3-030-23633-5_182019
Peixoto, Gislayne C. X., Lima, Gabriela L., Maia, Keila M., Souza, Ana Liza P., Castelo, Thibério S., Paiva, Ariana L. C., Paula, Valeria V., Oliveira, Moacir F., Brito, Adriel B., Domingues, Sheyla F. S., Viana, Ana Clara N. P. C. S., Melo, Luciana M., Comizzoli, Pierre, and Silva, Alexandre R. 2019. "Single injection of eCG/hCG leads to successful estrous synchronization in the collared peccary (Pecari tajacu Linnaeus, 1758)." Animal Reproduction Science 208:Article 106112. https://doi.org/10.1016/j.anireprosci.2019.1061122019
Paulson, Richard J. and Comizzoli, Pierre. 2018. "Addressing challenges in developing and implementing successful in vitro fertilization in endangered species: an opportunity for humanity to "give back"." Fertility and sterility 109 (3):418-419. https://doi.org/10.1016/j.fertnstert.2018.01.0312018
Della Togna, Gina, Gratwicke, Brian, Evans, Matthew, Augustine, Lauren, Chia, Han, Bronikowski, Ed, Murphy, James B., and Comizzoli, Pierre. 2018. "Influence of extracellular environment on the motility and structural properties of spermatozoa collected from hormonally stimulated Panamanian Golden Frog (Atelopus zeteki)." Theriogenology 108:153-160. https://doi.org/10.1016/j.theriogenology.2017.11.0322018
Lee, P. C., Wildt, David E., and Comizzoli, Pierre. 2018. "Proteomic analysis of germinal vesicles in the domestic cat model reveals candidate nuclear proteins involved in oocyte competence acquisition." Molecular Human Reproduction 24 (1):14-26. https://doi.org/10.1093/molehr/gax0592018
Comizzoli, Pierre, Paulson, E. E., and McGinnis, L. K. 2018. "The mutual benefits of research in wild animal species and human-assisted reproduction." Journal of assisted reproduction and genetics 1-10. https://doi.org/10.1007/s10815-018-1136-22018
Place, Ned J., Crosier, Adrienne E., Comizzoli, Pierre, Nagashima, Jennifer B., Haefele, Holly, Schmidt-Küntzel, Anne, and Marker, Laurie L. 2017. "Age-associated and deslorelin-induced declines in serum anti-Müllerian hormone concentrations in female cheetahs, Acinonyx jubatus." General and comparative endocrinology 250:54-57. https://doi.org/10.1016/j.ygcen.2017.06.0032017
Thuwanut, P., Tipkantha, W., Siriaroonrat, B., Comizzoli, Pierre, and Chatdarong, K. 2017. "Beneficial effect of extracellular adenosine 5'-triphosphate treatment on the Indochinese leopard (Panthera pardus delacouri) sperm quality after cryopreservation." Reproduction in Domestic Animals 52 (Supplement S2):269-274. https://doi.org/10.1111/rda.128542017
Crosier, Adrienne E., Comizzoli, Pierre, Koester, Diana C., and Wildt, David E. 2017. "Circumventing the natural, frequent oestrogen waves of the female cheetah (Acinonyx jubatus) using oral progestin (Altrenogest)." Reproduction Fertility and Development 29 (8):1486-1498. https://doi.org/10.1071/RD160072017
Rowlison, Tricia M., Ottinger, Mary Ann, and Comizzoli, Pierre. 2017. "Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat." Reproduction in Domestic Animals 52 (Supplement S2):193-196. https://doi.org/10.1111/rda.128312017
Patrick, Jennifer, Comizzoli, Pierre, and Elliott, Gloria. 2017. "Dry Preservation of Spermatozoa: Considerations for Different Species." Biopreservation and Biobanking 15 (2):158-168. https://doi.org/10.1089/bio.2016.00872017
Songsasen, Nucharin, Henson, Lh, Tipkantha, W., Thongkittidilok, C., Henson, Jh, Chatdarong, K., and Comizzoli, Pierre. 2017. "Dynamic changes in mitochondrial DNA, distribution and activity within cat oocytes during folliculogenesis." Reproduction in Domestic Animals 52 (Supplement S2):71-76. https://doi.org/10.1111/rda.128512017
Rowlison, Tricia M., Ottinger, Mary Ann, and Comizzoli, Pierre. 2017. "Key factors enhancing sperm fertilizing ability are transferred from the epididymis to the spermatozoa via epididymosomes in the domestic cat model." Journal of assisted reproduction and genetics 35 (2):221-228. https://doi.org/10.1007/s10815-017-1083-32017
Tipkantha, W., Thuwanut, P., Siriaroonrat, B., Comizzoli, Pierre, and Chatdarong, K. 2017. "Mitigation of sperm tail abnormalities using demembranation approach in the clouded leopard (Neofelis nebulosa)." Reproduction in Domestic Animals 52 (Supplement S2):214-218. https://doi.org/10.1111/rda.128612017
Koester, Diana C., Wildt, David E., Maly, Morgan, Comizzoli, Pierre, and Crosier, Adrienne E. 2017. "Non-invasive identification of protein biomarkers for early pregnancy diagnosis in the cheetah (Acinonyx jubatus)." PloS One 12 (12):1-21. https://doi.org/10.1371/journal.pone.01885752017
Mouttham, Lara L. and Comizzoli, Pierre. 2017. "Presence of sucrose in the vitrification solution and exposure for longer periods of time improve post-warming follicle integrity in cat ovarian tissues." Reproduction in Domestic Animals 52 (Supplement S2):224-229. https://doi.org/10.1111/rda.128472017
Tilker, Andrew, Long, Barney, Gray, Thomas N. E., Robichaud, William, Van Ngoc, Thinh, Vu Linh, Nguyen, Holland, Jeff, Shurter, Stephen, Comizzoli, Pierre, Thomas, Patrick, Ratajszczak, Radoslaw, and Burton, James. 2017. "Saving the saola from extinction." Science 357 (6357):1247-1247. https://doi.org/10.1126/science.aap95912017
Songsasen, Nucharin, Thongkittidilok, Chommanart, Yamamizu, K., Wildt, David E., and Comizzoli, Pierre. 2017. "Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model." Theriogenology 90:228-236. https://doi.org/10.1016/j.theriogenology.2016.12.0062017
Thuwanut, Paweena, Comizzoli, Pierre, Wildt, David E., Keefer, Carol L., and Songsasen, Nucharin. 2017. "Stem cell factor promotes in vitro ovarian follicle development in the domestic cat by upregulating c-kit mRNA expression and stimulating the phosphatidylinositol 3-kinase/AKT pathway." Reproduction Fertility and Development 29 (7):1356-1368. https://doi.org/10.1071/RD160712017
Tipkantha, Wanlaya, Thuwanut, Paweena, Maikeaw, Umaporn, Thongphakdee, Ampika, Yapila, Saifon, Kamolnorranath, Sumate, Siriaroonrat, Boripat, Comizzoli, Pierre, and Chatdarong, Kaywalee. 2017. "Successful laparoscopic oviductal artificial insemination in the clouded leopard (neofelis nebulosa) in thailand." Journal of Zoo and Wildlife Medicine 48 (3):804-812. https://doi.org/10.1638/2016-0287.12017
Thongphakdee, Ampika, Berg, Debra K., Tharasanit, Theerawat, Thongtip, Nikorn, Tipkantha, Wanlaya, Punkong, Chainarong, Tongthainan, Daraka, Noimoon, Sakhon, Maikeaw, Umaporn, Kajornklin, Nudthakamol, Siriaroonrat, Boripat, Comizzoli, Pierre, and Kamolnorranath, Sumate. 2017. "The impact of ovarian stimulation protocol on oocyte quality, subsequent in vitro embryo development, and pregnancy after transfer to recipients in Eld's deer (Rucervus eldii thamin)." Theriogenology 91:134-144. https://doi.org/10.1016/j.theriogenology.2016.12.0212017
Morselli, M. G., Luvoni, G. C., and Comizzoli, Pierre. 2017. "The nuclear and developmental competence of cumulus-oocyte complexes is enhanced by three-dimensional coculture with conspecific denuded oocytes during in vitro maturation in the domestic cat model." Reproduction in Domestic Animals 52 (Supplement S2):82-87. https://doi.org/10.1111/rda.128502017
Comizzoli, Pierre. 2016. "Advanced biotechnologies for wildlife fertility preservation." Thai Journal of Veterinary Medicine 46 (4):541-545.
2016
Herrick, Jason R., Mastromonaco, Gabriela, Songsasen, Nucharin, and Comizzoli, Pierre. 2016. "Biomaterials repositories: the science and business of biobanking." Reproduction, Fertility and Development 28 (8):https://doi.org/10.1071/RDv28n8_FO2016
Tanpradit, Nae, Chatdarong, Kaywalee, and Comizzoli, Pierre. 2016. "Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model." Journal of assisted reproduction and genetics 33 (12):1621-1631. https://doi.org/10.1007/s10815-016-0810-52016
Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2016. "Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis." Journal of Assisted Reproduction and Genetics 33 (6):783-794. https://doi.org/10.1007/s10815-016-0706-42016
Tipkantha, W., Thuwanut, Paweena, Morrell, J. M., Comizzoli, Pierre, and Chatdarong, Kaywalee. 2016. "Influence of living status (single vs. paired) and centrifugation with colloids on the sperm morphology and functionality in the clouded leopard (Neofelis nebulosa)." Theriogenology 86 (9):2202-2209. https://doi.org/10.1016/j.theriogenology.2016.07.0202016
Mouttham, Lara L. and Comizzoli, Pierre. 2016. "The preservation of vital functions in cat ovarian tissues during vitrification depends more on the temperature of the cryoprotectant exposure than on the sucrose supplementation." Cryobiology 73 (2):187-195. https://doi.org/10.1016/j.cryobiol.2016.07.0132016
Comizzoli, Pierre. 2015. "Biobanking efforts and new advances in male fertility preservation for rare and endangered species." Asian Journal of Andrology 17 (4):640-645. https://doi.org/10.4103/1008-682X.1538492015
Mouttham, Lara L., Fortune, Joanne E., and Comizzoli, Pierre. 2015. "Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture." Journal of assisted reproduction and genetics 32 (8):1239-1250. https://doi.org/10.1007/s10815-015-0543-x2015
Thuwanut, Paweena, Arya, Nlin, Comizzoli, Pierre, and Chatdarong, Kaywalee. 2015. "Effect of extracellular adenosine 5’- triphosphate (ATPe) on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality and in vitro fertilizing ability." Theriogenology 84 (5):702-709. https://doi.org/10.1016/j.theriogenology.2015.05.0032015
Lee, Pei-Chih, Wildt, David E., and Comizzoli, Pierre. 2015. "Nucleolar Translocation of Histone Deacetylase 2 Is Involved in Regulation of Transcriptional Silencing in the Cat Germinal Vesicle." Biology of reproduction 93 (2):33. https://doi.org/10.1095/biolreprod.115.1291062015
Tanpradit, Nae, Comizzoli, Pierre, Srisuwatanasagul, Sayamon, and Chatdarong, Kaywalee. 2015. "Positive impact of sucrose supplementation during slow freezing of cat ovarian tissues on cellular viability, follicle morphology, and DNA integrity." Theriogenology 83 (9):1553-1561. https://doi.org/10.1016/j.theriogenology.2015.01.0352015
Buarpung, Sirirak, Tharasanit, Theerawat, Thongkittidilok, Chommanart, Comizzoli, Pierre, and Techakumphu, Mongkol. 2015. "Spermatozoa isolated from cat testes retain their structural integrity as well as a developmental potential after refrigeration for up to 7 days." Zygote 23 (5):644-651. https://doi.org/10.1017/S09671994140002762015
Fujihara, Mayako, Comizzoli, Pierre, Keefer, Carol L., Wildt, David E., and Songsasen, Nucharin. 2014. "Epidermal Growth Factor (EGF) Sustains In Vitro Primordial Follicle Viability by Enhancing Stromal Cell Proliferation via MAPK and PI3K Pathways in the Prepubertal, but Not Adult Cat Ovary." Biology of Reproduction 90 (4):https://doi.org/10.1095/biolreprod.113.1150892014
Karakaya, Cengiz, Guzeloglu-Kayisli, Ozlem, Hobbs, Rebecca J., Gerasimova, Tsilya, Uyar, Asli, Erdem, Mehmet, Oktem, Mesut, Erdem, Ahmet, Gumuslu, Seyhan, Ercan, Deniz, Sakkas, Denny, Comizzoli, Pierre, Seli, Emre, and Lalioti, Maria D. 2014. "Follicle stimulating hormone receptor (FSHR) alternative skipping of exon 2 or 3 affects ovarian response to FSH." Molecular Human Reproduction 20 (7):630-643. https://doi.org/10.1093/molehr/gau0242014
Comizzoli, Pierre and Wildt, David E. 2014. "Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options." Reproduction, Fertility and Development 26 (1):91-98. https://doi.org/10.1071/RD132592014
Comizzoli, Pierre and Holt, William V. 2014. "Recent advances and prospects in germplasm preservation of rare and endangered species." Advances in Experimental Medicine and Biology 753:331-356. https://doi.org/10.1007/978-1-4939-0820-2_142014
Graves-Herring, Jennifer, Wildt, David E., and Comizzoli, Pierre. 2013. "Retention of Structure and Function of the Cat Germinal Vesicle after Air-Drying and Storage at Supra-Zero Temperature." Biology of Reproduction 88 (6):1-7. https://doi.org/10.1095/biolreprod.113.1084722013
Hobbs, Rebecca Jane, Comizzoli, Pierre, Howard, JoGayle, and Wildt, David E. 2012. "Absence of Seasonal Changes in FSHR Gene Expression in the Cat Cumulus-Oocyte Complex in Vivo and in Vitro." Reproduction 144:111-122. https://doi.org/10.1530/REP-12-00982012
Tharasanit, T., Buarpung, S., Manee-In, S., Thongkittidilok, C., Tiptanavattana, N., Comizzoli, Pierre, and Techakumphu, M. 2012. "Birth of Kittens After the Transfer of Frozen–Thawed Embryos Produced by Intracytoplasmic Sperm Injection with Spermatozoa Collected from Cryopreserved Testicular Tissue." Reproduction in Domestic Animals 47 (Supplement S6):305-308. https://doi.org/10.1111/rda.120722012
Fujihara, Mayako, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2012. "Cat and Dog Primordial Follicles Enclosed in Ovarian Cortex Sustain Viability after In vitro Culture on Agarose Gel in a Protein-Free Medium." Reproduction in Domestic Animals 47 (Supplement S6):102-108. https://doi.org/10.1111/rda.120222012
Comizzoli, Pierre and Wildt, David E. 2012. "Centrosomal Functions and Dysfunctions in Cat Spermatozoa." in The Centrosome, edited by Schatten, Heide., 49-58. Humana Press.
2012
Comizzoli, Pierre, Songsasen, Nucharin, Hagedorn, Mary M., and Wildt, David E. 2012. "Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species." Theriogenology 78 (8):1666-1681. https://doi.org/10.1016/j.theriogenology.2012.04.0082012
Buarpung, Sirirak, Tharasanit, Theerawat, Comizzoli, Pierre, and Techakumphu, Mongkol. 2012. "Effects of cold storage on plasma membrane, DNA integrity and fertilizing ability of feline testicular spermatozoa." Animal Reproduction Science 131 (3-4):219-227. https://doi.org/10.1016/j.anireprosci.2012.03.0112012
Buarpung, S., Tharasanit, T., Comizzoli, Pierre, and Techakumphu, M. 2012. "Feline spermatozoa from fresh and cryopreserved testicular tissues have comparable ability to fertilize matured oocytes and sustain the embryo development after intracytoplasmic sperm injection." Theriogenology 79 (1):149-158. https://doi.org/10.1016/j.theriogenology.2012.09.0222012
Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2012. "Increase in Histone Methylation in the Cat Germinal Vesicle Related to Acquisition of Meiotic and Developmental Competence." Reproduction in Domestic Animals 47 (Supplement S6):210-214. https://doi.org/10.1111/rda.120522012
Comizzoli, Pierre and Wildt, David E. 2012. "On the Horizon for Fertility Preservation in Domestic and Wild Carnivores." Reproduction in Domestic Animals 47 (Supplement S6):261-265. https://doi.org/10.1111/rda.120102012
Nestle, E., Graves-Herring, Jennifer, Keefer, C., and Comizzoli, Pierre. 2012. "Source of Protein Supplementation during In Vitro Culture does not Affect the Quality of Resulting Blastocysts in the Domestic Cat." Reproduction in Domestic Animals 47 (Supplement S6):152-155. https://doi.org/10.1111/rda.120472012
Songsasen, Nucharin, Comizzoli, Pierre, Nagashima, Jennifer B., Fujihara, Mayako, and Wildt, David E. 2012. "The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro." Reproduction in Domestic Animals 47 (Supplement S6):13-18. https://doi.org/10.1111/rda.120672012
Pukazhenthi, Budhan S., Hagedorn, Mary M., Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2011. "Cryopreserving endangered species gametes, embryos and gonadal tissue: Challenges, successes and future directions." Cryobiology 63 (3):308-309. https://doi.org/10.1016/j.cryobiol.2011.09.0142011
Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E., and Pukazhenthi, Budhan S. 2010. "Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus)." Theriogenology 73 (1):112-119. https://doi.org/10.1016/j.theriogenology.2009.08.0072010
Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2010. "Protecting and Extending Fertility for Females of Wild and Endangered Mammals." in Oncofertility, 87-100. Springer US.
2010
Comizzoli, Pierre, Crosier, Adrienne E., Songsasen, Nucharin, Szykman, Micaela, Howard, JoGayle, and Wildt, David E. 2009. "Advances in reproductive science for wild carnivore conservation." Reproduction in Domestic Animals 44:47-52.
2009
Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes." Reproduction in Domestic Animals 44:269-274.
2009
Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan S., and Songsasen, Nucharin. 2009. "Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health." Molecular reproduction and development 77 (5):397-409. https://doi.org/10.1002/mrd.211372009
Godard, N. M., Pukazhenthi, Budhan S., Wildt, David E., and Comizzoli, Pierre. 2009. "Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model." Fertility and sterility 91 (5):2051-2060. https://doi.org/10.1016/j.fertnstert.2008.05.0692009
Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2008. "Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat." Molecular reproduction and development 75:345-354.
2008
chapter
Comizzoli, Pierre and Power, Michael L. 2019. "Reproductive Microbiomes in Wild Animal Species: A New Dimension in Conservation Biology." in Reproductive Sciences in Animal Conservation, edited by Comizzoli, Pierre, Brown, Janine L., and Holt, W. V., 2nd ed. 225-240. Springer International Publishing AG. https://doi.org/10.1007/978-3-030-23633-5_82019
Songsasen, Nucharin and Comizzoli, Pierre. 2009. "A historic overview of embryos and oocyte preservation in the world of mammalian in vitro fertilization and biotechnology." in Preservation of Human Oocytes, edited by Borini, A. and Coticchio, G., 1-11. Informa Healthcare.
2009
Wang, Shangping, Lee, Pei-Chih, Elsayed, Amanda, Zhang, Fan, Zhang, Yong, Comizzoli, Pierre, and Elliott, Gloria D. 2021. "Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles." Cellular and Molecular Bioengineering, 14, (1) 101–112. https://doi.org/10.1007/s12195-020-00635-y.
2021
Amelkina, Olga and Comizzoli, Pierre. 2020. "Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model." Bmc Genomics, 21, (1). https://doi.org/10.1186/s12864-020-07236-z.
2020
Campos, Livia B., Praxedes, Erica C. G., Saraiva, Marcia V. A., Comizzoli, Pierre, and Silva, Alexandre R. 2019. "Advances and Challenges of Using Ovarian Preantral Follicles to Develop Biobanks of Wild Mammals." Biopreservation and Biobanking, 17, (4) 334–341. https://doi.org/10.1089/bio.2018.0130.
2019
Rodrigues Silva, Herlon Victor, Silva, Alexandre Rodrigues, Machado da Silvada, Lucia Daniel, and Comizzoli, Pierre. 2019. "Semen Cryopreservation and Banking for the Conservation of Neotropical Carnivores." Biopreservation and Biobanking, 17, (2) 183–188. https://doi.org/10.1089/bio.2018.0104.
2019
Comizzoli, Pierre. 2017. "Biobanking and fertility preservation for rare and endangered species." Animal Reproduction, 14, (1) 30–33. https://doi.org/10.21451/1984-3143-AR889.
2017
Comizzoli, Pierre and Holt, William V. 2016. "Implications of the Nagoya Protocol for genome resource banks composed of biomaterials from rare and endangered species." Reproduction Fertility and Development, 28, (8) 1145–1151. https://doi.org/10.1071/RD15429.
2016