Comizzoli, Pierre
Research Biologist - Program Officer for Science
Comizzoli, Pierre
Research Biologist - Program Officer for Science
Reproductive physiology and biotechnologies in Ungulates and Carnivores (domestic and wild).
Multi-disciplinary research.
Geographic Focus
Background And Education
Education And Training
- D.V.M., Veterinary School of Maisons-Alfort
- M.Sc., University of Paris VI
- Ph.D., University of Tours
Awards And Honors
- Smithsonian Scholarly Studies Award, conferred by Smithsonian Scholarly Studies Awards, 2020
- Smithsonian Scholarly Studies Award, conferred by Smithsonian Scholarly Studies Awards, 2019
- Smithsonian Scholarly Studies Award, conferred by Smithsonian Scholarly Studies Awards, 2018
- Smithsonian Scholarly Studies Award, conferred by Smithsonian Scholarly Studies Awards, 2017
Public Biography
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Dr. Pierre Comizzoli has worked as a veterinarian in French Guyana and in the African Sahelo-Saharan region. He also has a master's degree from the University of Paris VI and a doctoral degree from the University of Tours on reproductive biotechnologies in bovine and deer species. During his doctoral studies, he described an original effect of the paternal component on early embryo development in both species, and produced in vitro the first transferable embryos in red deer and Japanese sika deer.
Dr. Comizzoli is a staff scientist at the National Zoo and Conservation Biology Institute since 2002. He develops new projects on gamete and gonadal tissue cryo-banking for rare and endangered species. His comparative research on fertility preservation in various wild and domestic animal species creates interesting bridges with human reproductive health and medicine.
Besides conducting research on cutting-edge approaches in fertility preservation and assisted reproduction, Dr. Comizzoli is also in charge of conservation projects on wild carnivores and ungulates in Northern Africa and in Southeast Asia. Dr. Comizzoli leads the Pan-Smithsonian Cryo-Initiative (PSCI) which aims to improve the management and use of biomaterial repositories within the entire Smithsonian. Since 2014, Dr. Comizzoli also works as a Senior Program Officer at the Office of the Under Secretary for Science and Research to develop pan-institutional efforts in One Health as well as in sustainability.
Research And Grants
Co-principal Investigator On
- Understanding sperm maturation to enhance male fertility in the domestic cat model awarded by Smithsonian Scholarly Studies Awards 2018 -
- Assessing and Improving the Preservation of Feline Oocytes Using Protein Markers awarded by Smithsonian Scholarly Studies Awards
- Diagnosing Pregnancy in the Cheetah - Cracking the code to understand fertility failure awarded by Competitive Grants Program for Science
- Genetic Distinctions Between Two Pairs of Closely-Related Canids and Felids: Developing Comparative Genomic and Evolutionary Foundations for Management and Conservation awarded by Competitive Grants Program for Science
- A novel in vitro approach to preserve and extend the female fertility in rare and endangered species awarded by Competitive Grants Program for Science
Investigator On
- Creation of a Mobile Laboratory Unit to Collect and Preserve Sperm and Eggs from the Endangered Panamanian Golden Frog (Atelopus zeteki) awarded by Woodtiger Fund
- Drying, Storing and Reanimating Egg Germinal Vesicles awarded by National Institutes of Health
- Drying, Storing and Reanimating Egg Germinal Vesicles to Preserve Fertility awarded by U.S. Department of Health and Human Services
- Expanding Laboratory Activities to Collect, Preserve, and Use Germplasms and Cell Lines from the Endangered Panamanian Golden Frog (Atelopus zeteki) awarded by Woodtiger Fund
- Understanding mechanisms of cellular stress response to develop long-term preservation of living tissues at room temperatures
- Using the Microbiome for Sustained Synthesis and Delivery of a Spermicide within the Vagina A Novel Approach to Non-Hormonal Contraception for Women awarded by Eastern Virginia Medical School
Publications
Selected Publications
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Article
- Bornbusch, Sally L., Bamford, Alexandra, Thacher, Piper, Crosier, Adrienne, Marinari, Paul, Bortner, Robyn, Garelle, Della, Livieri, Travis, Santymire, Rachel, Comizzoli, Pierre, Maslanka, Michael, Maldonado, Jesús E., Koepfli, Klaus-Peter, Muletz-Wolz, Carly, and DeCandia, Alexandra L. 2024. "Author Correction: Markers of fertility in reproductive microbiomes of male and female endangered black-footed ferrets (Mustela nigripes)." Communications Biology, 7, (1). https://doi.org/10.1038/s42003-024-06793-3. 2024
- Cowl, Veronica B., Comizzoli, Pierre, Appeltant, Ruth, Bolton, Rhiannon L., Browne, Robert, Holt, William V., Penfold, Linda M., Swegen, Aleona, Walker, Susan L., and Williams, Suzannah A. 2024. "Cloning for the Twenty-First Century and Its Place in Endangered Species Conservation." Annual Review of Animal Biosciences, 12, (1). https://doi.org/10.1146/annurev-animal-071423-093523. 2024
- Bezerra, Luana Grasiele Pereira, de Oliveira,Radan, Elvis Matias, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2024. "Collection, Handling, and Preservation of Wild Bird Semen: Current Status, Challenges, and Perspectives." Biopreservation and Biobanking, 22, (3) 191–210. https://doi.org/10.1089/bio.2023.0053. 2024
- Sosnicki, Danielle M., Travis, Alexander J., and Comizzoli, Pierre. 2024. "Extracellular vesicles are involved in the paracrine communication between epithelial cells in different regions of the domestic cat epididymis." Biology of reproduction, https://doi.org/10.1093/biolre/ioae129. 2024
- Bornbusch, Sally L., Bamford, Alexandra, Thacher, Piper, Crosier, Adrienne, Marinari, Paul, Bortner, Robyn, Garelle, Della, Livieri, Travis, Santymire, Rachel, Comizzoli, Pierre, Maslanka, Michael, Maldonado, Jesús E., Koepfli, Klaus-Peter, Muletz-Wolz, Carly, and DeCandia, Alexandra L. 2024. "Markers of fertility in reproductive microbiomes of male and female endangered black-footed ferrets (Mustela nigripes)." Communications Biology, 7, (1) 224. https://doi.org/10.1038/s42003-024-05908-0. 2024
- Lee, Pei-Chih and Comizzoli, Pierre. 2024. "Microwave-assisted dehydration, long-term storage at non-freezing temperatures, and rehydration of cat germinal vesicles." Biology of reproduction, https://doi.org/10.1093/biolre/ioae060. 2024
- Hagedorn, Mary, Parenti, Lynne R., Craddock, Robert A., Comizzoli, Pierre, Mabee, Paula, Meinke, Bonnie, Wolf, Susan M., Bischof, John C., Sandlin, Rebecca D., Tessier, Shannon N., and Toner, Mehmet. 2024. "Safeguarding Earth's biodiversity by creating a lunar biorepository." Bioscience, https://doi.org/10.1093/biosci/biae058. 2024
- Ali, Nadya, Amelkina, Olga, Santymire, Rachel M., Koepfli, Klaus-Peter, Comizzoli, Pierre, and Vazquez, Juan M. 2024. "Semen proteome and transcriptome of the endangered black-footed ferret (Mustela nigripes) show association with the environment and fertility outcome." Scientific Reports, 14, (7063). https://doi.org/10.1038/s41598-024-57096-w. 2024
- da Silva, Andreia Maria, Pereira, Ana Gloria, Bezerra, Luana Graziele Pereira, Brasil, Andreza Vieira, Pereira, Alexsandra Fernandes, de Oliveira, Moacir Franco, Rodrigues, Ana Paula Ribeiro, Naupas, Lucy Vanessa Sulca, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2024. "Synergistic effects of glial cell line-derived neurotrophic factor and base-medium on in vitro culture of testicular tissue derived from prepubertal collared peccary." Cell biology international, https://doi.org/10.1002/cbin.12203. 2024
- Bezerra, Luana G. P., Silva, Andréia M., Jurema, Artur P., Dantas, Maiko R. T., Pereira, Ana G., Oliveira, Moacir F., Comizzoli, Pierre, and Silva, Alexandre R. 2023. "Changes in Sperm Morphology, Morphometry, and Motility from the Epididymis to the Vas Deferens in Rheas (Rhea americana, Linnaeus, 1758)." Animals, 13, (9). https://doi.org/10.3390/ani13091483. 2023
- Comizzoli, Pierre, Amelkina, Olga, Chavez, Daniela R., Rowlison, Tricia R., and Lee, Pei-Chih. 2023. "Current knowledge in the biology of gametes and embryos from Carnivora." Theriogenology, 196 254–263. https://doi.org/10.1016/j.theriogenology.2022.11.024. 2023
- Bezerra, Luana Grasiele Pereira, Silva, Andréia Maria, Dantas, Maiko Roberto Tavares, dos Santos, Romário Parente, Moreira, Samara Sandy Jeronimo, Pereira, Ana Glória, de Oliveira, Moacir Franco, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2023. "Development of assays for the characterization of sperm motility parameters, viability, and membrane integrity in the epididymis and vas deferens of the greater rhea (Rhea americana)." Animal Reproduction, 20, (4). https://doi.org/10.1590/1984-3143-AR2023-0113. 2023
- DeCandia, Alexandra L., Adeduro, Laura, Thacher, Piper, Crosier, Adrienne, Marinari, Paul, Bortner, Robyn, Garelle, Della, Livieri, Travis, Santymire, Rachel, Comizzoli, Pierre, Maslanka, Michael, Maldonado, Jesús E., Koepfli, Klaus-Peter, Muletz-Wolz, Carly, and Bornbusch, Sally L. 2023. "Gut bacterial composition shows sex-specific shifts during breeding season in ex situ managed black-footed ferrets." Journal of Heredity, https://doi.org/10.1093/jhered/esad065. 2023
- Kaganer, Alyssa W., Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. "Immune priming prior to pathogen exposure sheds light on the relationship between host, microbiome and pathogen in disease." Royal Society Open Science, 10, (2). https://doi.org/10.1098/rsos.220810. 2023
- Comizzoli, Pierre, Luvoni, Cecilia, and Kutzler, Michelle. 2023. "Introduction to the special issue 'Canine and Feline Reproduction: Reproductive biology and medicine of domestic and exotic carnivores'." Theriogenology, 200 77–78. https://doi.org/10.1016/j.theriogenology2023.01.017. 2023
- Comizzoli, Pierre, Luvoni, Cecilia, and Kutzler, Michelle. 2023. "Introduction to the special issue ‘Canine and Feline Reproduction: Reproductive biology and medicine of domestic and exotic carnivores’." Theriogenology, 200 77–78. https://doi.org/10.1016/j.theriogenology.2023.01.017. 2023
- Sosnicki, Danielle M., Cohen, Roy, Asano, Atsushi, Nelson, Jacquelyn L., Mukai, Chinatsu, Comizzoli, Pierre, and Travis, Alexander J. 2023. "Segmental differentiation of the murine epididymis: identification of segment-specific, GM1-enriched vesicles and regulation by luminal fluid factors." Biology of reproduction, 109, (6) 864–877. https://doi.org/10.1093/biolre/ioad120. 2023
- O’Mahony, S. M. and Comizzoli, Pierre. 2023. "Special series on the role of the microbiome in reproduction and fertility." Reproduction and Fertility, 4, (4). https://doi.org/10.1530/RAF-23-0080. 2023
- Orozco Cabral, Joseph A., Lee, Pei-Chih, Wang, Shangping, Wang, Yizhou, Zhang, Yong, Comizzoli, Pierre, and Elliott, Gloria D. 2023. "The Effect of Choline Salt Addition to Trehalose Solution for Long-Term Storage of Dried and Viable Nuclei from Fully Grown Oocytes." Bioengineering, 10, (9). https://doi.org/10.3390/bioengineering10091000. 2023
- Rowlison, Tricia and Comizzoli, Pierre. 2023. "Transfer of Galectin-3-Binding Protein via Epididymal Extracellular Vesicles Promotes Sperm Fertilizing Ability and Developmental Potential in the Domestic Cat Model." International Journal of Molecular Sciences, 24, (4). https://doi.org/10.3390/ijms24043077. 2023
- Lee, Pei-Chih, Stewart, Samantha, Amelkina, Olga, Sylvester, Hannah, He, Xiaoming, and Comizzoli, Pierre. 2023. "Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying." Journal of assisted reproduction and genetics, 40, (8) 1817–1828. https://doi.org/10.1007/s10815-023-02831-x. 2023
- Silva, Alexandre Rodrigues, Pereira, Alexsandra Fernandes, and Comizzoli, Pierre. 2022. "Biobanking and use of gonadal tissues - a promising strategy for conserving wildlife from the Caatinga biome." Animal Reproduction, 19, (4). https://doi.org/10.1590/1984-3143-ar2022-0135. 2022
- Crosier, Adrienne E., Byron, M. J., and Comizzoli, Pierre. 2022. "Connecting the spots: Understanding cheetah reproduction to improve assisted breeding and population management." Theriogenology, 185 70–77. https://doi.org/10.1016/j.theriogenology.2022.03.025. 2022
- Silva, Andreia M., Pereira, Ana G., Bezerra, Luana G. P., Jeronimo Moreira, Samara S., Pereira, Alexsandra F., Oliveira, Moacir F., Comizzoli, Pierre, and Silva, Alexandre R. 2022. "Cryopreservation of Testicular Tissue from Adult Red-Rumped Agoutis (Dasyprocta leporina Linnaeus, 1758)." Animals, 12, (6). https://doi.org/10.3390/ani12060738. 2022
- Comizzoli, Pierre, Amelkina, Olga, and Lee, Pei-Chih. 2022. "Damages and stress responses in sperm cells and other germplasms during dehydration and storage at nonfreezing temperatures for fertility preservation." Molecular reproduction and development, https://doi.org/10.1002/mrd.23651. 2022
- Rowlison, Tricia, Nagashima, Jennifer, Acker, Jason Paul, Ben, Robert, Daly, Jonathan, Hagedorn, Mary, and Comizzoli, Pierre. 2022. "First Report of Successful Laser Warming for Frozen Gonadal Tissues and Oocytes in the Domestic Cat Model." Biopreservation and Biobanking, https://doi.org/10.1089/bio.2022.0071. 2022
- Macente, Beatrice Ingrid, Fonseca-Alves, Carlos Eduardo, Magalhaes, Georgia Mode, Tavares, Mariana Riboli, Mansano, Cleber Fernando Menegasso, Mouttham, Lara, Apparicio, Maricy, Toniollo, Gilson Helio, and Comizzoli, Pierre. 2022. "Influence of Vitrification Device, Warming Protocol, and Subsequent In Vitro Culture on Structural Integrity of Testicular Fragments from Adult Domestic Cats." Biopreservation and Biobanking, https://doi.org/10.1089/bio.2021.0092. 2022
- Moreira, Samara S. J., Santos, Caio S., Castelo, Thibério S., Bezerra, Luana G. P., Praxedes, Érica C. G., Matos, Tayná M., Souza-Junior, Jo, Feijó, Francisco M. C., Comizzoli, Pierre, and Silva, Alexandre R. 2022. "Investigating the need for antibiotic supplementation to the extender used for semen cryopreservation in collared peccaries." Frontiers in Veterinary Science, 9. https://doi.org/10.3389/fvets.2022.954921. 2022
- Comizzoli, Pierre, He, Xiaoming, and Lee, Pei-Chih. 2022. "Long-term preservation of germ cells and gonadal tissues at ambient temperatures." Reproduction and Fertility, 3, (2) R42–R50. https://doi.org/10.1530/RAF-22-0008. 2022
- Comizzoli, Pierre, Loi, Pasqualino, Patrizio, Pasquale, and Hubel, Allison. 2022. "Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?" Journal of assisted reproduction and genetics, https://doi.org/10.1007/s10815-021-02392-x. 2022
- Howell, Lachlan G., Mawson, Peter R., Comizzoli, Pierre, Witt, Ryan R., Frankham, Richard, Clulow, Simon, O'Brien, Justine K., Clulow, John, Marinari, Paul, and Rodger, John C. 2022. "Modeling genetic benefits and financial costs of integrating biobanking into the conservation breeding of managed marsupials." Conservation Biology, https://doi.org/10.1111/cobi.14010. 2022
- Holt, William, V. and Comizzoli, Pierre. 2022. "Opportunities and Limitations for Reproductive Science in Species Conservation." Annual Review of Animal Biosciences, 10 491–511. https://doi.org/10.1146/annurev-animal-013120-030858. 2022
- Comizzoli, Pierre and Holt, William V. 2022. "Recent Progress in Spermatology Contributing to the Knowledge and Conservation of Rare and Endangered Species." Annual Review of Animal Biosciences, 10, (1) 469–490. https://doi.org/10.1146/annurev-animal-020420-040600. 2022
- Pomeroy, Kimball O., Comizzoli, Pierre, Rushing, John S., Lersten, Ivy L., and Nel-Themaat, Liesl. 2022. "The ART of cryopreservation and its changing landscape." Fertility and sterility, 117, (3) 469–476. https://doi.org/10.1016/j.fertnstert.2022.01.018. 2022
- Comizzoli, Pierre. 2021. "Adding new ingredients to the recipe for successful embryo transfers." Journal of assisted reproduction and genetics, https://doi.org/10.1007/s10815-021-02148-7. 2021
- Lee, Pei-Chih, Zahmel, Jennifer, Jewgenow, Katarina, and Comizzoli, Pierre. 2021. "Desiccated cat spermatozoa retain DNA integrity and developmental potential after prolonged storage and shipping at non-cryogenic temperatures." Journal of assisted reproduction and genetics, https://doi.org/10.1007/s10815-021-02337-4. 2021
- Paulson, Erika Elinor and Comizzoli, Pierre. 2021. "Endometrial receptivity and embryo implantation in carnivores-commonalities and differences with other mammalian species." Biology of reproduction, 104, (4) 771–783. https://doi.org/10.1093/biolre/ioab001. 2021
- Rowlison, Tricia, Ottinger, Mary Ann, and Comizzoli, Pierre. 2021. "Exposure to epididymal extracellular vesicles enhances immature sperm function and sustains vitality of cryopreserved spermatozoa in the domestic cat model." Journal of assisted reproduction and genetics, https://doi.org/10.1007/s10815-021-02214-0. 2021
- da Silva, Andreia Maria, Pereira, Ana Gloria, Brasil, Andreza Vieira, Macedo, Lua Barbalho, Souza-Junior, Joao, Bezerra de Moura, Carlos Eduardo, Pereira, Alexsandra Fernandes, de Oliveira, Moacir Franco, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2021. "Influence of freezing techniques and glycerol-based cryoprotectant combinations on the survival of testicular tissues from adult collared peccaries." Theriogenology, 167 111–119. https://doi.org/10.1016/j.theriogenology.2021.03.013. 2021
- Comizzoli, Pierre, Power, Michael L., Bornbusch, Sally L., and Muletz-Wolz, Carly. 2021. "Interactions between reproductive biology and microbiomes in wild animal species." Animal Microbiome, 3, (1) 87. https://doi.org/10.1186/s42523-021-00156-7. 2021
- Chavez, Daniela R., Lee, Pei-Chih, and Comizzoli, Pierre. 2021. "Oocyte Meiotic Competence in the Domestic Cat Model: Novel Roles for Nuclear Proteins BRD2 and NPM1." Frontiers in Cell and Developmental Biology, 9. https://doi.org/10.3389/fcell.2021.670021. 2021
- Wang, Shangping, Lee, Pei-Chih, Elsayed, Amanda, Zhang, Fan, Zhang, Yong, Comizzoli, Pierre, and Elliott, Gloria D. 2021. "Preserving the Female Genome in Trehalose Glass at Supra-Zero Temperatures: The Relationship Between Moisture Content and DNA Damage in Feline Germinal Vesicles." Cellular and Molecular Bioengineering, 14, (1) 101–112. https://doi.org/10.1007/s12195-020-00635-y. 2021
- Zainuddin, Zainal Zahari, Sipangkui, Symphorosa, Kelana, Mohd Farqhan, Chee, Yap Keng, Tarmizi, Mohamed Reza Mohamed, and Comizzoli, Pierre. 2021. "Repeated Evaluations of Testes and Semen Characteristics in Two Binturongs (Arctictis binturong)." Frontiers in Veterinary Science, 8. https://doi.org/10.3389/fvets.2021.658573. 2021
- Jiang, Bin, Li, Weijie, Stewart, Samantha, Ou, Wenquan, Liu, Baolin, Comizzoli, Pierre, and He, Xiaoming. 2021. "Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells." Bioactive Materials, 6, (12) 4377–4388. https://doi.org/10.1016/j.bioactmat.2021.04.025. 2021
- Comizzoli, Pierre, Pagenkopp Lohan, Katrina M., Muletz-Wolz, Carly, Hassell, James, and Coyle, Brian J. 2021. "The Interconnected Health Initiative: A Smithsonian Framework to Extend One Health Research and Education." Frontiers in Veterinary Science, 8. https://doi.org/10.3389/fvets.2021.629410. 2021
- Rowlison, Tricia and Comizzoli, Pierre. 2021. "The Knowns and Unknowns about Epididymal Extracellular Vesicles in Different Animal Species." Advanced Biology, https://doi.org/10.1002/adbi.202101066. 2021
- Amelkina, Olga, da Silva, Andreia M., Silva, Alexandre R., and Comizzoli, Pierre. 2021. "Transcriptome dynamics in developing testes of domestic cats and impact of age on tissue resilience to cryopreservation." Bmc Genomics, 22, (1). https://doi.org/10.1186/s12864-021-08099-8. 2021
- Comizzoli, Pierre and Ottinger, Mary Ann. 2021. "Understanding Reproductive Aging in Wildlife to Improve Animal Conservation and Human Reproductive Health." Frontiers in Cell and Developmental Biology, 9. https://doi.org/10.3389/fcell.2021.680471. 2021
- Le Gac, Séverine, Ferraz, Marcia, Venzac, Bastien, and Comizzoli, Pierre. 2021. "Understanding and Assisting Reproduction in Wildlife Species Using Microfluidics." Trends in biotechnology, 39, (6) 584–597. https://doi.org/10.1016/j.tibtech.2020.08.012. 2021
- Comizzoli, Pierre. 2020. "A sweet and steady future---long-term storage of gametes and gonadal tissues in trehalose glass at room temperature." F&S Science, 1, (2) 113–114. https://doi.org/10.1016/j.xfss.2020.09.002. 2020
- Comizzoli, Pierre. 2020. "Birth of a Giant Panda Cub After Artificial Insemination with Frozen–Thawed Semen: A Powerful Reminder About the Key Role of Biopreservation and Biobanking for Wildlife Conservation." Biopreservation and Biobanking, 18, (5). https://doi.org/10.1089/bio.2020.29076.pjc. 2020
- Silva, Andréia Maria da, Pereira, Alexsandra Fernandes, Comizzoli, Pierre, and Silva, Alexandre Rodrigues. 2020. "Cryopreservation and Culture of Testicular Tissues: An Essential Tool for Biodiversity Preservation." Biopreservation and Biobanking, 18, (3) 235–243. https://doi.org/10.1089/bio.2020.0010. 2020
- Pukazhenthi, Budhan, Songsasen, Nucharin, Brown, Janine, Comizzoli, Pierre, Crosier, Adrienne, Hagedorn, Mary, Gratwicke, Brian, Pitt, William, and Monfort, Steven. 2020. "David E. Wildt-An Inspiring Leader in the Conservation of Wild Species." Journal of Heredity, 111, (4) 414–416. https://doi.org/10.1093/jhered/esaa013. 2020
- Crosier, Adrienne E., Lamy, Julie, Bapodra, Priya, Rapp, Suzi, Maly, Morgan, Junge, Randy, Haefele, Holly, Ahistus, Jason, Santiestevan, Jenny, and Comizzoli, Pierre. 2020. "First Birth of Cheetah Cubs from In Vitro Fertilization and Embryo Transfer." Animals, 10, (10). https://doi.org/10.3390/ani10101811. 2020
- Zainuddin, Zainal Zahari, Mohamed Tarmizi, Mohamed Reza, Yap, Keng Chee, Comizzoli, Pierre, and Sipangkui, Symphorosa. 2020. "First Evaluations and Cryopreservation of Semen Samples from Sunda Clouded Leopards (Neofelis diardi)." Animals, 10, (6) Article 1072. https://doi.org/10.3390/ani10061072. 2020
- Silva, Herlon Victor Rodrigues, da Silva, Andréia Maria, Lee, Pei-Chih, Brito, Bruna Farias, Silva, Alexandre Rodrigues, da Silva, Lúcia,Daniel Machado, and Comizzoli, Pierre. 2020. "Influence of Microwave-Assisted Drying on Structural Integrity and Viability of Testicular Tissues from Adult and Prepubertal Domestic Cats." Biopreservation and Biobanking, https://doi.org/10.1089/bio.2020.0048. 2020
- Silva, Herlon Victor Rodrigues, Nunes, Thalles Gothardo Pereira, Brito, Bruna Farias, Campos, Lí, da Silva, Andréia Maria, Silva, Alexandre Rodrigues, Comizzoli, Pierre, and da Silva, Lúcia Daniel Machado. 2020. "Influence of different extenders on morphological and functional parameters of frozen-thawed spermatozoa of jaguar (Panthera onca)." Cryobiology, 92 53–61. https://doi.org/10.1016/j.cryobiol.2019.10.195. 2020
- Amelkina, Olga and Comizzoli, Pierre. 2020. "Initial response of ovarian tissue transcriptome to vitrification or microwave-assisted dehydration in the domestic cat model." Bmc Genomics, 21, (1). https://doi.org/10.1186/s12864-020-07236-z. 2020
- Comizzoli, Pierre. 2020. "Integrating fertility preservation and cryo-banking into the conservation of rare and endangered deer species." Animal Production Science, 60, (10) 1227–1232. https://doi.org/10.1071/AN18674. 2020
- Lima, David Baruc Cruvinel, da Silva, Lucia Daniel Machado, Marinari, Paul, and Comizzoli, Pierre. 2020. "Long-Term Preservation of Testicular Tissue Integrity and Viability Using Vitrification in the Endangered Black-Footed Ferret (Mustela nigripes)." Animals, 10, (10) 1865–1865. https://doi.org/10.3390/ani10101865. 2020
- Campbell, Genevieve E., Jones, Estella L., Comizzoli, Pierre, and Duffy, Diane M. 2020. "Neurotensin stimulates the sperm acrosome reaction and reduces percentages of fertilization in vitro." F&S Science, 1, (1) 27–35. https://doi.org/10.1016/j.xfss.2020.03.001. 2020
- Rowlison, Tricia, Cleland, Timothy P., Ottinger, Mary Ann, and Comizzoli, Pierre. 2020. "Novel proteomic profiling of epididymal extracellular vesicles in the domestic cat reveals proteins related to sequential sperm maturation with differences observed between normospermic and teratospermic individuals." Molecular & Cellular Proteomics, 19, (12) 2090–2103. https://doi.org/10.1074/mcp.RA120.002251. 2020
- Thongphakdee, Ampika, Sukparangsi, Woranop, Comizzoli, Pierre, and Chatdarong, Kaywalee. 2020. "Reproductive biology and biotechnologies in wild felids." Theriogenology, 150 360–373. https://doi.org/10.1016/j.theriogenology.2020.02.004. 2020
- Stabach, Jared A., Cunningham, Stephanie A., Connette, Grant, Mota, Joel L., Reed, Dolores, Byron, Michael, Songer, Melissa, Wacher, Tim, Mertes, Katherine, Brown, Janine L., Comizzoli, Pierre, Newby, John, Monfort, Steven L., and Leimgruber, Peter. 2020. "Short-term effects of GPS collars on the activity, behavior, and adrenal response of scimitar-horned oryx (Oryx dammah)." PloS One, 15, (2) Article e0221843. https://doi.org/10.1371/journal.pone.0221843. 2020
- Cramer, Emily, Krauss, Nicole, Rowlison, Tricia, and Comizzoli, Pierre. 2020. "Sperm Morphology and Male Age in Black-Throated Blue Warblers, an Ecological Model System." Animals, 10, (7) Article 1175. https://doi.org/10.3390/ani10071175. 2020
- Thuwanut, Paweena, Comizzoli, Pierre, Pruksananonda, Kamthorn, Chatdarong, Kaywalee, and Songsasen, Nucharin. 2019. "Activation of adenosine monophosphate-activated protein kinase (AMPK) enhances energy metabolism, motility, and fertilizing ability of cryopreserved spermatozoa in domestic cat model." Journal of assisted reproduction and genetics, 36, (7) 1401–1412. https://doi.org/10.1007/s10815-019-01470-5. 2019
- Campos, Livia B., Praxedes, Erica C. G., Saraiva, Marcia V. A., Comizzoli, Pierre, and Silva, Alexandre R. 2019. "Advances and Challenges of Using Ovarian Preantral Follicles to Develop Biobanks of Wild Mammals." Biopreservation and Biobanking, 17, (4) 334–341. https://doi.org/10.1089/bio.2018.0130. 2019
- Comizzoli, Pierre and Holt, William V. 2019. "Breakthroughs and new horizons in reproductive biology of rare and endangered animal species." Biology of reproduction, 101, (3) 514–525. https://doi.org/10.1093/biolre/ioz031. 2019
- Zhou, Ran, Wildt, David E., Keefer, Carol L., and Comizzoli, Pierre. 2019. "Combinations of Growth Factors Regulating LIF/STAT3, WNT, and FGF2 Pathways Sustain Pluripotency-Related Proteins in Cat Embryonic Cells." Stem Cells and Development, 28, (5). https://doi.org/10.1089/scd.2018.0109. 2019
- Lee, Pei-Chih and Comizzoli, Pierre. 2019. "Desiccation and supra-zero temperature storage of cat germinal vesicles lead to less structural damage and similar epigenetic alterations compared to cryopreservation." Molecular Reproduction & Development, 86, (12) 1822–1831. https://doi.org/10.1002/mrd.23276. 2019
- Mbemya, Gildas Tetaping, de Sá, Naiza Arcângela Ribeiro, Guerreiro, Denise Damasceno, de Sousa, Francisca,Geovania Canaf, Nguedia, Sylvain Njina, Alves, Benner Geraldo, Santos, Francielli Weber, Pessoa, Otília Deusdênia Loiola, Comizzoli, Pierre, Figueiredo, José Ricardo, and Rodrigues, Ana Paula Ribeiro. 2019. "Early ovine preantral follicles have a potential to grow until antral stage in two-step culture system in the presence of aqueous extract of Justicia insularis." Reproduction in domestic animals = Zuchthygiene, 1–35. https://doi.org/10.1111/rda.13477. 2019
- Zhou, Ran, Comizzoli, Pierre, and Keefer, Carol L. 2019. "Endogenous pluripotent factor expression after reprogramming cat fetal fibroblasts using inducible transcription factors." Molecular reproduction and development, 86, (11) 1671–1681. https://doi.org/10.1002/mrd.23257. 2019
- Maia, K. M., Souza, A. L. P., Silva, A. M., Souza-, J. B. F., Jr., Costa, L. L. M., Brandao, F. Z., Oliveira, M. F., Comizzoli, Pierre, and Silva, A. R. 2019. "Environmental effects on collared peccaries (Pecan tajacu) serum testosterone, testicular morphology, and semen quality in the Caatinga biome." Theriogenology, 126 286–294. https://doi.org/10.1016/j.theriogenology.2018.12.032. 2019
- Bowley, Sarah C., Comizzoli, Pierre, Lindell, Kevin A., Matsas, David J., and White, Eugene C. 2019. "Genetic Cryopreservation of Rare Breeds of Domesticated North American Livestock: Smithsonian & SVF Biodiversity Preservation Project." Diversity, 11, (10) 198. https://doi.org/10.3390/d11100198. 2019
- Amstislavsky, Sergei, Mokrousova, Valentina, Brusentsev, Eugeny, Okotrub, Konstantin, and Comizzoli, Pierre. 2019. "Influence of Cellular Lipids on Cryopreservation of Mammalian Oocytes and Preimplantation Embryos: A Review." Biopreservation and Biobanking, 17, (1). https://doi.org/10.1089/bio.2018.0039. 2019
- Lee, Pei-Chih, Adams, Daniella M., Amelkina, Olga, White, Kylie K., Amoretti, Luigi A., Whitaker, Marinda G., and Comizzoli, Pierre. 2019. "Influence of microwave-assisted dehydration on morphological integrity and viability of cat ovarian tissues: First steps toward long-term preservation of complex biomaterials at supra-zero temperatures." PloS One, 14, (12) Article e0225440. https://doi.org/10.1371/journal.pone.0225440. 2019
- Wilsterman, Kathryn, Bentley, George E., and Comizzoli, Pierre. 2019. "RFRP3 influences basal lamina degradation, cellular death, and progesterone secretion in cultured preantral ovarian follicles from the domestic cat." PeerJ, 7 e7540. https://doi.org/10.7717/peerj.7540. 2019
- Comizzoli, Pierre, Brown, Janine L., and Holt, William V. 2019. "Reproductive Science as an Essential Component of Conservation Biology: New Edition." Advances in Experimental Medicine and Biology, 1200 1–10. https://doi.org/10.1007/978-3-030-23633-5_1. 2019
- Thuwanut, Paweena, Brown, Janine L., Comizzoli, Pierre, and Crosier, Adrienne E. 2019. "Responsiveness of the cheetah (Acinonyx jubatus) ovary to exogenous gonadotropins after preemptive oral progestin treatment." Theriogenology, 138 39–46. https://doi.org/10.1016/j.theriogenology.2019.07.001. 2019
- Rodrigues Silva, Herlon Victor, Silva, Alexandre Rodrigues, Machado da Silvada, Lucia Daniel, and Comizzoli, Pierre. 2019. "Semen Cryopreservation and Banking for the Conservation of Neotropical Carnivores." Biopreservation and Biobanking, 17, (2) 183–188. https://doi.org/10.1089/bio.2018.0104. 2019
- Wilsterman, Kathryn, Bao, Xinmiao, Estrada, Allegra D., Comizzoli, Pierre, and Bentley, George E. 2019. "Sex steroids influence organizational but not functional decidualization of feline endometrial cells in a 3D culture system." Biology of reproduction, 101, (5) 906–915. https://doi.org/10.1093/biolre/ioz145. 2019
- Peixoto, Gislayne C. X., Lima, Gabriela L., Maia, Keila M., Souza, Ana Liza P., Castelo, Thibério S., Paiva, Ariana L. C., Paula, Valeria V., Oliveira, Moacir F., Brito, Adriel B., Domingues, Sheyla F. S., Viana, Ana Clara N. P. C. S., Melo, Luciana M., Comizzoli, Pierre, and Silva, Alexandre R. 2019. "Single injection of eCG/hCG leads to successful estrous synchronization in the collared peccary (Pecari tajacu Linnaeus, 1758)." Animal Reproduction Science, 208 Article 106112. https://doi.org/10.1016/j.anireprosci.2019.106112. 2019
- Comizzoli, Pierre. 2019. "Superficial and profound considerations about sperm quality." Journal of assisted reproduction and genetics, 36, (7) 1361–1362. https://doi.org/10.1007/s10815-019-01506-w. 2019
- Dundon, Morgan, Madden, Odile, and Comizzoli, Pierre. 2019. "Three-dimensional culture of endometrial cells from domestic cats: A new in vitro platform for assessing plastic toxicity." PLOS ONE, 14, (5) 1–15. https://doi.org/10.1371/journal.pone.0217365. 2019
- Cramer, Emily R. A., Rowlison, Tricia, Comizzoli, Pierre, and Ryder, T. Brandt. 2019. "Uniform Sperm Morphology in the Lek-Breeding Wire-Tailed Manakin (Pipra Filicauda)." Ornitologia Neotropical, 30 135–139. 2019
- Koepfli, Klaus-Peter, Tamazian, Gaik, Wildt, David E., Dobrynin, Pavel, Kim, Changhoon, Frandsen, Paul B., Godinho, Raquel, Yurchenko, Andrey A., Komissarov, Aleksey, Krasheninnikova, Ksenia, Kliver, Sergei, Kolchanova, Sofia, Gonçalves, Margarida, Carneiro, Miguel, Vaz Pinto, Pedro, Ferrand, Nuno, Maldonado, Jesús E., Ferrie, Gina M., Chemnick, Leona, Ryder, Oliver A., Johnson, Warren E., Comizzoli, Pierre, O'Brien, Stephen J., and Pukazhenthi, Budhan S. 2019. "Whole Genome Sequencing and Re-sequencing of the Sable Antelope (Hippotragus niger): A Resource for Monitoring Diversity in ex Situ and in Situ Populations." G3 (Bethesda, Md.), 9, (6) 1785–1793. https://doi.org/10.1534/g3.119.400084. 2019
- Paulson, Richard J. and Comizzoli, Pierre. 2018. "Addressing challenges in developing and implementing successful in vitro fertilization in endangered species: an opportunity for humanity to "give back"." Fertility and sterility, 109, (3) 418–419. https://doi.org/10.1016/j.fertnstert.2018.01.031. 2018
- Della Togna, Gina, Gratwicke, Brian, Evans, Matthew, Augustine, Lauren, Chia, Han, Bronikowski, Ed, Murphy, James B., and Comizzoli, Pierre. 2018. "Influence of extracellular environment on the motility and structural properties of spermatozoa collected from hormonally stimulated Panamanian Golden Frog (Atelopus zeteki)." Theriogenology, 108 153–160. https://doi.org/10.1016/j.theriogenology.2017.11.032. 2018
- Lima, David Baruc Cruvinel, Silva, Lú, and Comizzoli, Pierre. 2018. "Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model." PloS One, 13, (11). https://doi.org/10.1371/journal.pone.0207317. 2018
- Thongkittidilok, Chommanart, Singh, Ram Pratap, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2018. "Insulin promotes preantral follicle growth and antrum formation through temporal expression of genes regulating steroidogenesis and water transport in the cat." Reproduction Fertility and Development, 30, (10) 1369–1379. https://doi.org/10.1071/RD17454. 2018
- Lee, P. C., Wildt, David E., and Comizzoli, Pierre. 2018. "Proteomic analysis of germinal vesicles in the domestic cat model reveals candidate nuclear proteins involved in oocyte competence acquisition." Molecular Human Reproduction, 24, (1) 14–26. https://doi.org/10.1093/molehr/gax059. 2018
- Fujihara, Mayako, Yamamizu, Kohei, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2018. "Retinoic acid promotes in vitro follicle activation in the cat ovary by regulating expression of matrix metalloproteinase 9." Plos One, 13, (8) 1–14. https://doi.org/10.1371/journal.pone.0202759. 2018
- Mbemya, Gildas Tetaping, Cadenas, Jesus, Ribeiro, de S., Damasceno Guerreiro, Denise, Donfack, Nathalie Jiatsa, Alberto Vieira, Luis, Canafístula, de Sousa, Geraldo Alves, Benner, Lobo, Carlos Henrique, Santos, Francielli Weber, Lima Pinto, Francisco,das Chagas, Loiola Pessoa, Otília Deusdênia, Smitz, Johan, Comizzoli, Pierre, Figueiredo, José Ricardo, and Rodrigues, Ana Paula Ribeiro. 2018. "Supplementation of in vitro culture medium with FSH to grow follicles and mature oocytes can be replaced by extracts of Justicia insularis." PloS One, 13, (12). https://doi.org/10.1371/journal.pone.0208760. 2018
- Comizzoli, Pierre, Paulson, E. E., and McGinnis, L. K. 2018. "The mutual benefits of research in wild animal species and human-assisted reproduction." Journal of assisted reproduction and genetics, 1–10. https://doi.org/10.1007/s10815-018-1136-2. 2018
- Place, Ned J., Crosier, Adrienne E., Comizzoli, Pierre, Nagashima, Jennifer B., Haefele, Holly, Schmidt-Küntzel, Anne, and Marker, Laurie L. 2017. "Age-associated and deslorelin-induced declines in serum anti-Müllerian hormone concentrations in female cheetahs, Acinonyx jubatus." General and comparative endocrinology, 250 54–57. https://doi.org/10.1016/j.ygcen.2017.06.003. 2017
- Thuwanut, P., Tipkantha, W., Siriaroonrat, B., Comizzoli, Pierre, and Chatdarong, K. 2017. "Beneficial effect of extracellular adenosine 5'-triphosphate treatment on the Indochinese leopard (Panthera pardus delacouri) sperm quality after cryopreservation." Reproduction in Domestic Animals, 52, (Supplement S2) 269–274. https://doi.org/10.1111/rda.12854. 2017
- Comizzoli, Pierre. 2017. "Biobanking and fertility preservation for rare and endangered species." Animal Reproduction, 14, (1) 30–33. https://doi.org/10.21451/1984-3143-AR889. 2017
- Crosier, Adrienne E., Comizzoli, Pierre, Koester, Diana C., and Wildt, David E. 2017. "Circumventing the natural, frequent oestrogen waves of the female cheetah (Acinonyx jubatus) using oral progestin (Altrenogest)." Reproduction Fertility and Development, 29, (8) 1486–1498. https://doi.org/10.1071/RD16007. 2017
- Rowlison, Tricia M., Ottinger, Mary Ann, and Comizzoli, Pierre. 2017. "Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat." Reproduction in Domestic Animals, 52, (Supplement S2) 193–196. https://doi.org/10.1111/rda.12831. 2017
- Patrick, Jennifer, Comizzoli, Pierre, and Elliott, Gloria. 2017. "Dry Preservation of Spermatozoa: Considerations for Different Species." Biopreservation and Biobanking, 15, (2) 158–168. https://doi.org/10.1089/bio.2016.0087. 2017
- Songsasen, Nucharin, Henson, Lh, Tipkantha, W., Thongkittidilok, C., Henson, Jh, Chatdarong, K., and Comizzoli, Pierre. 2017. "Dynamic changes in mitochondrial DNA, distribution and activity within cat oocytes during folliculogenesis." Reproduction in Domestic Animals, 52, (Supplement S2) 71–76. https://doi.org/10.1111/rda.12851. 2017
- Della Togna, Gina, Trudeau, Vance L., Gratwicke, Brian, Evans, Matthew, Augustine, Lauren, Chia, Han, Bronikowski, Edward J., Murphy, James B., and Comizzoli, Pierre. 2017. "Effects of hormonal stimulation on the concentration and quality of excreted spermatozoa in the critically endangered Panamanian golden frog (Atelopus zeteki)." Theriogenology, 91 27–35. https://doi.org/10.1016/j.theriogenology.2016.12.033. 2017
- Rowlison, Tricia M., Ottinger, Mary Ann, and Comizzoli, Pierre. 2017. "Key factors enhancing sperm fertilizing ability are transferred from the epididymis to the spermatozoa via epididymosomes in the domestic cat model." Journal of assisted reproduction and genetics, 35, (2) 221–228. https://doi.org/10.1007/s10815-017-1083-3. 2017
- Tipkantha, W., Thuwanut, P., Siriaroonrat, B., Comizzoli, Pierre, and Chatdarong, K. 2017. "Mitigation of sperm tail abnormalities using demembranation approach in the clouded leopard (Neofelis nebulosa)." Reproduction in Domestic Animals, 52, (Supplement S2) 214–218. https://doi.org/10.1111/rda.12861. 2017
- Koester, Diana C., Wildt, David E., Maly, Morgan, Comizzoli, Pierre, and Crosier, Adrienne E. 2017. "Non-invasive identification of protein biomarkers for early pregnancy diagnosis in the cheetah (Acinonyx jubatus)." PloS One, 12, (12) 1–21. https://doi.org/10.1371/journal.pone.0188575. 2017
- Mouttham, Lara L. and Comizzoli, Pierre. 2017. "Presence of sucrose in the vitrification solution and exposure for longer periods of time improve post-warming follicle integrity in cat ovarian tissues." Reproduction in Domestic Animals, 52, (Supplement S2) 224–229. https://doi.org/10.1111/rda.12847. 2017
- Tilker, Andrew, Long, Barney, Gray, Thomas N. E., Robichaud, William, Van Ngoc, Thinh, Vu Linh, Nguyen, Holland, Jeff, Shurter, Stephen, Comizzoli, Pierre, Thomas, Patrick, Ratajszczak, Radoslaw, and Burton, James. 2017. "Saving the saola from extinction." Science, 357, (6357) 1247–1247. https://doi.org/10.1126/science.aap9591. 2017
- Songsasen, Nucharin, Thongkittidilok, Chommanart, Yamamizu, K., Wildt, David E., and Comizzoli, Pierre. 2017. "Short-term hypertonic exposure enhances in vitro follicle growth and meiotic competence of enclosed oocytes while modestly affecting mRNA expression of aquaporin and steroidogenic genes in the domestic cat model." Theriogenology, 90 228–236. https://doi.org/10.1016/j.theriogenology.2016.12.006. 2017
- Thuwanut, Paweena, Comizzoli, Pierre, Wildt, David E., Keefer, Carol L., and Songsasen, Nucharin. 2017. "Stem cell factor promotes in vitro ovarian follicle development in the domestic cat by upregulating c-kit mRNA expression and stimulating the phosphatidylinositol 3-kinase/AKT pathway." Reproduction Fertility and Development, 29, (7) 1356–1368. https://doi.org/10.1071/RD16071. 2017
- Patrick, Jennifer L., Elliott, Gloria D., and Comizzoli, Pierre. 2017. "Structural integrity and developmental potential of spermatozoa following microwave-assisted drying in the domestic cat model." Theriogenology, 103 36–43. https://doi.org/10.1016/j.theriogenology.2017.07.037. 2017
- Tipkantha, Wanlaya, Thuwanut, Paweena, Maikeaw, Umaporn, Thongphakdee, Ampika, Yapila, Saifon, Kamolnorranath, Sumate, Siriaroonrat, Boripat, Comizzoli, Pierre, and Chatdarong, Kaywalee. 2017. "Successful laparoscopic oviductal artificial insemination in the clouded leopard (neofelis nebulosa) in thailand." Journal of Zoo and Wildlife Medicine, 48, (3) 804–812. https://doi.org/10.1638/2016-0287.1. 2017
- Thongphakdee, Ampika, Berg, Debra K., Tharasanit, Theerawat, Thongtip, Nikorn, Tipkantha, Wanlaya, Punkong, Chainarong, Tongthainan, Daraka, Noimoon, Sakhon, Maikeaw, Umaporn, Kajornklin, Nudthakamol, Siriaroonrat, Boripat, Comizzoli, Pierre, and Kamolnorranath, Sumate. 2017. "The impact of ovarian stimulation protocol on oocyte quality, subsequent in vitro embryo development, and pregnancy after transfer to recipients in Eld's deer (Rucervus eldii thamin)." Theriogenology, 91 134–144. https://doi.org/10.1016/j.theriogenology.2016.12.021. 2017
- Morselli, M. G., Luvoni, G. C., and Comizzoli, Pierre. 2017. "The nuclear and developmental competence of cumulus-oocyte complexes is enhanced by three-dimensional coculture with conspecific denuded oocytes during in vitro maturation in the domestic cat model." Reproduction in Domestic Animals, 52, (Supplement S2) 82–87. https://doi.org/10.1111/rda.12850. 2017
- Comizzoli, Pierre. 2016. "Advanced biotechnologies for wildlife fertility preservation." Thai Journal of Veterinary Medicine, 46, (4) 541–545. https://doi.org/10.56808/2985-1130.2772. 2016
- Herrick, Jason R., Mastromonaco, Gabriela, Songsasen, Nucharin, and Comizzoli, Pierre. 2016. "Biomaterials repositories: the science and business of biobanking." Reproduction, Fertility and Development, 28, (8). https://doi.org/10.1071/RDv28n8_FO. 2016
- Tanpradit, Nae, Chatdarong, Kaywalee, and Comizzoli, Pierre. 2016. "Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (FCCP) pre-exposure ensures follicle integrity during in vitro culture of ovarian tissue but not during cryopreservation in the domestic cat model." Journal of assisted reproduction and genetics, 33, (12) 1621–1631. https://doi.org/10.1007/s10815-016-0810-5. 2016
- Comizzoli, Pierre and Holt, William V. 2016. "Implications of the Nagoya Protocol for genome resource banks composed of biomaterials from rare and endangered species." Reproduction Fertility and Development, 28, (8) 1145–1151. https://doi.org/10.1071/RD15429. 2016
- Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2016. "Incidence of methylated histones H3K4 and H3K79 in cat germinal vesicles is regulated by specific nuclear factors at the acquisition of developmental competence during the folliculogenesis." Journal of Assisted Reproduction and Genetics, 33, (6) 783–794. https://doi.org/10.1007/s10815-016-0706-4. 2016
- Tipkantha, W., Thuwanut, Paweena, Morrell, J. M., Comizzoli, Pierre, and Chatdarong, Kaywalee. 2016. "Influence of living status (single vs. paired) and centrifugation with colloids on the sperm morphology and functionality in the clouded leopard (Neofelis nebulosa)." Theriogenology, 86, (9) 2202–2209. https://doi.org/10.1016/j.theriogenology.2016.07.020. 2016
- Mouttham, Lara L. and Comizzoli, Pierre. 2016. "The preservation of vital functions in cat ovarian tissues during vitrification depends more on the temperature of the cryoprotectant exposure than on the sucrose supplementation." Cryobiology, 73, (2) 187–195. https://doi.org/10.1016/j.cryobiol.2016.07.013. 2016
- Comizzoli, Pierre. 2015. "Biobanking efforts and new advances in male fertility preservation for rare and endangered species." Asian Journal of Andrology, 17, (4) 640–645. https://doi.org/10.4103/1008-682X.153849. 2015
- Comizzoli, Pierre. 2015. "Biotechnologies for wildlife fertility preservation." Animal Frontiers, 5, (1) 73–78. https://doi.org/10.2527/af.2015-0011. 2015
- Mouttham, Lara L., Fortune, Joanne E., and Comizzoli, Pierre. 2015. "Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture." Journal of assisted reproduction and genetics, 32, (8) 1239–1250. https://doi.org/10.1007/s10815-015-0543-x. 2015
- Thuwanut, Paweena, Arya, Nlin, Comizzoli, Pierre, and Chatdarong, Kaywalee. 2015. "Effect of extracellular adenosine 5’- triphosphate (ATPe) on cryopreserved epididymal cat sperm intracellular ATP concentration, sperm quality and in vitro fertilizing ability." Theriogenology, 84, (5) 702–709. https://doi.org/10.1016/j.theriogenology.2015.05.003. 2015
- Lee, Pei-Chih, Wildt, David E., and Comizzoli, Pierre. 2015. "Nucleolar Translocation of Histone Deacetylase 2 Is Involved in Regulation of Transcriptional Silencing in the Cat Germinal Vesicle." Biology of reproduction, 93, (2) 33. https://doi.org/10.1095/biolreprod.115.129106. 2015
- Tanpradit, Nae, Comizzoli, Pierre, Srisuwatanasagul, Sayamon, and Chatdarong, Kaywalee. 2015. "Positive impact of sucrose supplementation during slow freezing of cat ovarian tissues on cellular viability, follicle morphology, and DNA integrity." Theriogenology, 83, (9) 1553–1561. https://doi.org/10.1016/j.theriogenology.2015.01.035. 2015
- Elliott, Gloria D., Lee, Pei-Chih, Paramore, Elisha, Van Vorst, Matthew, and Comizzoli, Pierre. 2015. "Resilience of Oocyte Germinal Vesicles to Microwave-Assisted Drying in the Domestic Cat Model." Biopreservation and Biobanking, 13, (3) 164–171. https://doi.org/10.1089/bio.2014.0078. 2015
- Buarpung, Sirirak, Tharasanit, Theerawat, Thongkittidilok, Chommanart, Comizzoli, Pierre, and Techakumphu, Mongkol. 2015. "Spermatozoa isolated from cat testes retain their structural integrity as well as a developmental potential after refrigeration for up to 7 days." Zygote, 23, (5) 644–651. https://doi.org/10.1017/S0967199414000276. 2015
- Fujihara, Mayako, Comizzoli, Pierre, Keefer, Carol L., Wildt, David E., and Songsasen, Nucharin. 2014. "Epidermal Growth Factor (EGF) Sustains In Vitro Primordial Follicle Viability by Enhancing Stromal Cell Proliferation via MAPK and PI3K Pathways in the Prepubertal, but Not Adult Cat Ovary." Biology of Reproduction, 90, (4). https://doi.org/10.1095/biolreprod.113.115089. 2014
- Karakaya, Cengiz, Guzeloglu-Kayisli, Ozlem, Hobbs, Rebecca J., Gerasimova, Tsilya, Uyar, Asli, Erdem, Mehmet, Oktem, Mesut, Erdem, Ahmet, Gumuslu, Seyhan, Ercan, Deniz, Sakkas, Denny, Comizzoli, Pierre, Seli, Emre, and Lalioti, Maria D. 2014. "Follicle stimulating hormone receptor (FSHR) alternative skipping of exon 2 or 3 affects ovarian response to FSH." Molecular Human Reproduction, 20, (7) 630–643. https://doi.org/10.1093/molehr/gau024. 2014
- Comizzoli, Pierre and Wildt, David E. 2014. "Mammalian fertility preservation through cryobiology: value of classical comparative studies and the need for new preservation options." Reproduction, Fertility and Development, 26, (1) 91–98. https://doi.org/10.1071/RD13259. 2014
- Comizzoli, Pierre and Holt, William V. 2014. "Recent advances and prospects in germplasm preservation of rare and endangered species." Advances in Experimental Medicine and Biology, 753 331–356. https://doi.org/10.1007/978-1-4939-0820-2_14. 2014
- Hribal, R., Jewgenow, K., Braun, B., and Comizzoli, Pierre. 2013. "Influence of Culture Medium Composition on Relative mRNA Abundances in Domestic Cat Embryos." Reproduction in Domestic Animals, 48, (2) 245–251. https://doi.org/10.1111/j.1439-0531.2012.02139.x. 2013
- Graves-Herring, Jennifer, Wildt, David E., and Comizzoli, Pierre. 2013. "Retention of Structure and Function of the Cat Germinal Vesicle after Air-Drying and Storage at Supra-Zero Temperature." Biology of Reproduction, 88, (6) 1–7. https://doi.org/10.1095/biolreprod.113.108472. 2013
- Hobbs, Rebecca Jane, Comizzoli, Pierre, Howard, JoGayle, and Wildt, David E. 2012. "Absence of Seasonal Changes in FSHR Gene Expression in the Cat Cumulus-Oocyte Complex in Vivo and in Vitro." Reproduction, 144 111–122. https://doi.org/10.1530/REP-12-0098. 2012
- Tharasanit, T., Buarpung, S., Manee-In, S., Thongkittidilok, C., Tiptanavattana, N., Comizzoli, Pierre, and Techakumphu, M. 2012. "Birth of Kittens After the Transfer of Frozen–Thawed Embryos Produced by Intracytoplasmic Sperm Injection with Spermatozoa Collected from Cryopreserved Testicular Tissue." Reproduction in Domestic Animals, 47, (Supplement S6) 305–308. https://doi.org/10.1111/rda.12072. 2012
- Fujihara, Mayako, Comizzoli, Pierre, Wildt, David E., and Songsasen, Nucharin. 2012. "Cat and Dog Primordial Follicles Enclosed in Ovarian Cortex Sustain Viability after In vitro Culture on Agarose Gel in a Protein-Free Medium." Reproduction in Domestic Animals, 47, (Supplement S6) 102–108. https://doi.org/10.1111/rda.12022. 2012
- Comizzoli, Pierre, Songsasen, Nucharin, Hagedorn, Mary M., and Wildt, David E. 2012. "Comparative cryobiological traits and requirements for gametes and gonadal tissues collected from wildlife species." Theriogenology, 78, (8) 1666–1681. https://doi.org/10.1016/j.theriogenology.2012.04.008. 2012
- Buarpung, Sirirak, Tharasanit, Theerawat, Comizzoli, Pierre, and Techakumphu, Mongkol. 2012. "Effects of cold storage on plasma membrane, DNA integrity and fertilizing ability of feline testicular spermatozoa." Animal Reproduction Science, 131, (3-4) 219–227. https://doi.org/10.1016/j.anireprosci.2012.03.011. 2012
- Buarpung, S., Tharasanit, T., Comizzoli, Pierre, and Techakumphu, M. 2012. "Feline spermatozoa from fresh and cryopreserved testicular tissues have comparable ability to fertilize matured oocytes and sustain the embryo development after intracytoplasmic sperm injection." Theriogenology, 79, (1) 149–158. https://doi.org/10.1016/j.theriogenology.2012.09.022. 2012
- Phillips, Tameka C., Wildt, David E., and Comizzoli, Pierre. 2012. "Increase in Histone Methylation in the Cat Germinal Vesicle Related to Acquisition of Meiotic and Developmental Competence." Reproduction in Domestic Animals, 47, (Supplement S6) 210–214. https://doi.org/10.1111/rda.12052. 2012
- Comizzoli, Pierre and Wildt, David E. 2012. "On the Horizon for Fertility Preservation in Domestic and Wild Carnivores." Reproduction in Domestic Animals, 47, (Supplement S6) 261–265. https://doi.org/10.1111/rda.12010. 2012
- Nestle, E., Graves-Herring, Jennifer, Keefer, C., and Comizzoli, Pierre. 2012. "Source of Protein Supplementation during In Vitro Culture does not Affect the Quality of Resulting Blastocysts in the Domestic Cat." Reproduction in Domestic Animals, 47, (Supplement S6) 152–155. https://doi.org/10.1111/rda.12047. 2012
- Songsasen, Nucharin, Comizzoli, Pierre, Nagashima, Jennifer B., Fujihara, Mayako, and Wildt, David E. 2012. "The Domestic Dog and Cat as Models for Understanding the Regulation of Ovarian Follicle Development In Vitro." Reproduction in Domestic Animals, 47, (Supplement S6) 13–18. https://doi.org/10.1111/rda.12067. 2012
- Pukazhenthi, Budhan S., Hagedorn, Mary M., Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2011. "Cryopreserving endangered species gametes, embryos and gonadal tissue: Challenges, successes and future directions." Cryobiology, 63, (3) 308–309. https://doi.org/10.1016/j.cryobiol.2011.09.014. 2011
- Crosier, Adrienne E., Comizzoli, Pierre, Baker, Tomas, Davidson, Autumn, Munson, Linda, Howard, JoGayle, Marker, Laurie L., and Wildt, David E. 2011. "Increasing Age Influences Uterine Integrity, but Not Ovarian Function or Oocyte Quality in the Cheetah (Acinonyx jubatus)." Biology of reproduction, 85, (2) 243–253. https://doi.org/10.1095/biolreprod.110.089417. 2011
- Comizzoli, Pierre, Pukazhenthi, Budhan S., and Wildt, David E. 2011. "The competence of germinal vesicle oocytes is unrelated to nuclear chromatin configuration and strictly depends on cytoplasmic quantity and quality in the cat model." Human Reproduction, 26, (8) 2165–2177. https://doi.org/10.1093/humrep/der176. 2011
- Siriaroonrat, B., Comizzoli, Pierre, Songsasen, Nucharin, Monfort, Steven L., Wildt, David E., and Pukazhenthi, Budhan S. 2010. "Oocyte quality and estradiol supplementation affect in vitro maturation success in the white-tailed deer (Odocoileus virginianus)." Theriogenology, 73, (1) 112–119. https://doi.org/10.1016/j.theriogenology.2009.08.007. 2010
- Comizzoli, Pierre, Crosier, Adrienne E., Songsasen, Nucharin, Szykman, Micaela, Howard, JoGayle, and Wildt, David E. 2009. "Advances in reproductive science for wild carnivore conservation." Reproduction in Domestic Animals, 44 47–52. 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2009. "In vitro compaction of germinal vesicle chromatin is beneficial to survival of vitrified cat oocytes." Reproduction in Domestic Animals, 44 269–274. 2009
- Wildt, David E., Comizzoli, Pierre, Pukazhenthi, Budhan S., and Songsasen, Nucharin. 2009. "Lessons from biodiversity - the value of nontraditional species to advance reproductive science, conservation, and human health." Molecular reproduction and development, 77, (5) 397–409. https://doi.org/10.1002/mrd.21137. 2009
- Godard, N. M., Pukazhenthi, Budhan S., Wildt, David E., and Comizzoli, Pierre. 2009. "Paracrine Factors from Cumulus-Enclosed Oocytes Ensure the Successful Maturation and Fertilization in Vitro of Denuded Oocytes in the Cat Model." Fertility and sterility, 91, (5) 2051–2060. https://doi.org/10.1016/j.fertnstert.2008.05.069. 2009
- Comizzoli, Pierre, Wildt, David E., and Pukazhenthi, Budhan S. 2008. "Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat." Molecular reproduction and development, 75 345–354. 2008
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Chapter
- Comizzoli, Pierre, Brown, Janine L., and Holt, William V. 2019. "From the Ivory Tower to Reality! Conclusions of the New Edition." In Reproductive Sciences in Animal Conservation. 2nd ed. Comizzoli, Pierre, Brown, Janine L., and Holt, William V., editors. 545–550. Cham, Switzerland: Springer International Publishing AG. In Advances in Experimental Medicine and Biology, 1200. https://doi.org/10.1007/978-3-030-23633-5_18. 2019
- Comizzoli, Pierre and Power, Michael L. 2019. "Reproductive Microbiomes in Wild Animal Species: A New Dimension in Conservation Biology." In Reproductive Sciences in Animal Conservation. 2nd ed. Comizzoli, Pierre, Brown, Janine L., and Holt, W. V., editors. 225–240. Cham, Switzerland: Springer International Publishing AG. In Advances in Experimental Medicine and Biology, 1200. https://doi.org/10.1007/978-3-030-23633-5_8. 2019
- Comizzoli, Pierre and Wildt, David E. 2012. "Centrosomal Functions and Dysfunctions in Cat Spermatozoa." In The Centrosome. Schatten, Heide, editor. 49–58. Humana Press. 2012
- Comizzoli, Pierre, Songsasen, Nucharin, and Wildt, David E. 2010. "Protecting and Extending Fertility for Females of Wild and Endangered Mammals." In Oncofertility. 87–100. Springer US. In Cancer Treatment and Research (vol. 156). 2010
- Songsasen, Nucharin and Comizzoli, Pierre. 2009. "A historic overview of embryos and oocyte preservation in the world of mammalian in vitro fertilization and biotechnology." In Preservation of Human Oocytes. Borini, A. and Coticchio, G., editors. 1–11. London: Informa Healthcare. 2009
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Dataset
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin Bd transcriptome BLAST nr annotations. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14773269.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin Bd transcriptome BLAST xml. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14773260.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin Bd transcriptome gene counts matrix. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14773236.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert, Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin Bd transcriptome metadata. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14773293.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin microbiome 16S Anti-Bd OTU list. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14750706.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin microbiome 16S OTU table. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14750622.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin microbiome 16S OTU tree. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14750682.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin microbiome 16S metadata. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14750703.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin prokaryote transcriptome. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14751009.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin prokaryote transcriptome BLAST nr annotations. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14751165.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin prokaryote transcriptome BLAST xml. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14751075.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin prokaryote transcriptome isoform counts matrix. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14751063.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14773326.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome BLAST nr annotations. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14786088.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome BLAST xml. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14786001.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome isoform counts matrix. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14785968.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome metadata. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14786094.V1. 2023
- Kaganer, Alyssa, Ossiboff, Robert J., Keith, Nicole I., Schuler, Krysten L., Comizzoli, Pierre, Hare, Matthew P., Fleischer, Robert C., Gratwicke, Brian, and Bunting, Elizabeth M. 2023. [Dataset] Hellbender skin transcriptome metadata. Distributed by Washington, DC: Smithsonian National Zoo and Conservation Biology Institute. https://doi.org/10.25573/DATA.14786094.V1. 2023
Activities
Responsible Collections Areas
- Frozen Biological Collections (Pan-Smithsonian Cryo-Initiative)
Contact
Location
- National Zoo & Conservation Biology Institute Academic Department
