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Low-copy nuclear markers in Isoëtes (Isoëtaceae) identified with transcriptomes

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Abstract

  • PREMISE OF THE STUDY: Few genetic markers provide phylogenetic information in closely related species of Isoetes (Isoetaceae). We describe the development of primers for several putative low-copy nuclear markers to resolve the phylogeny of Isoetes, particularly in the southeastern United States. METHODS AND RESULTS: We identified regions of interest in Isoetes transcriptomes based on low-copy genes in other plants. Primers were designed for these regions and tested with 16 taxa of Isoetes and one species of Lycopodium. Parts of the pgiC, gapC, and IBR3 gene regions show phylogenetic signal within the North American and Mediterranean clades of Isoetes. Conclusions: Transcriptome data prove useful for identification and primer design of low-copy genes. Three new markers show potential for inferring phylogenies in regional clades of Isoetes, and possibly across the entire genus.

Publication Date

  • 2018

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