Background Here I revise the Neotropical genus Rhabdotylus Lutz, 1913 (Tabaninae: Diachlorini), including redescription of three species that range from Guatemala to Argentina: Rhabdotylus rubrum (Thunberg, 1827), Rhabdotylus venenatum (Osten Sacken, 1886), and Rhabdotylus viridiventris (Macquart, 1838). New information Rhabdotylus planiventris (Wiedemann, 1828) is established as a junior synonym of R. rubrum, syn. nov. A dichotomous identification key based on external morphological characters is provided.