We sequenced the complete mitochondrial genome of Hamamelistes spinosus using the shotgun genome-skimming method. The mitogenome of H. spinosus is 15,089 bp in length with a much higher A T content of 81.8% than that of G C (18.2%) and consists of 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and 1 control region. All the 13 PCGs initiate with a typical ATN and end with TAA except for two genes COX1 and ND4, which terminate with a single T. All the tRNAs form a classical clover-leaf secondary structure except for tRNA-Ser (AGN), which does not have the dihydrouridine (DHU) arm. In addition, we constructed the phylogenetic tree of Aphididae with two species Adelges laricis and Daktulosphaira vitifoliae as outgroups and the results showed that Hamamelistes spinosus is sister to Hormaphis betulae and they are closely allied with Neothoracaphis yanonis.
