We isolated eight novel polymorphic microsatellite loci from Pteridium aquilinum. These loci were characterized in 30 individuals, one from Bolivia, two from Peru, one from the USA, one from Japan, and 25 from Northeast China to Southwest China. The number of alleles per locus ranged from two to seven. The observed heterozygosity (HO) ranged from 0.000 to 0.600 with an average of 0.3051, and the expected heterozygosity (HE) ranged from 0.0966 to 0.7780 with an average of 0.4267. One locus deviated from Hardy2013Weinberg equilibrium and four pairs of loci were found to be in linkage disequilibrium. These polymorphic loci will be useful in the study of the population genetic structure of Pteridium.
