Nagashima, J. B., Travis, A. J., and Songsasen, Nucharin. 2019. "The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer." In Comparative Embryo Culture: Methods and Protocols. Herrick, Jason R., editor. 247–267. New York: Springer. In Methods in Molecular Biology.
Advances in embryo technologies in the domestic dog have made significant strides in the past decade. This progress has been spurred by interests in taking advantage of the dog as a biomedical research model for human and companion animal medicine, developing assisted reproductive technologies to manage genetic diversity in endangered canids maintained ex situ, and improving breeding in rare or working breeds of dogs. Here, we focus on recent advancements and techniques for collection of in vivo-matured oocytes, in vitro fertilization (IVF), in vitro culture of early (≤8-cell) and advanced stage (≥16-cell) embryos, and embryo transfer.
