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Complete Genomic Sequence of Bacteriophage Felix O1

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Complete Citation

  • Whichard, Jean M., Weigt, Lee A., Borris, Douglas J., Li, Ling Ling, Zhang, Qing, Kapur, Vivek, Pierson, F. William, Lingohr, Erika J., She, Yi-Min, Kropinski, Andrew M., and Sriranganathan, Nammalwar. 2010. "Complete Genomic Sequence of Bacteriophage Felix O1." Viruses, 2, (3) 710–730. https://doi.org/10.3390/v2030710.

Overview

Abstract

  • Bacteriophage O1 is a Myoviridae A1 group member used historically for identifying Salmonella. Sequencing revealed a single, linear, 86,155-base-pair genome with 39% average G C content, 131 open reading frames, and 22 tRNAs. Closest protein homologs occur in Erwinia amylovora phage phi Ea21-4 and Escherichia coli phage wV8. Proteomic analysis indentified structural proteins: Gp23, Gp36 (major tail protein), Gp49, Gp53, Gp54, Gp55, Gp57, Gp58 (major capsid protein), Gp59, Gp63, Gp64, Gp67, Gp68, Gp69, Gp73, Gp74 and Gp77 (tail fiber). Based on phage-host codon differences, 7 tRNAs could affect translation rate during infection. Introns, holin-lysin cassettes, bacterial toxin homologs and host RNA polymerase-modifying genes were absent.

Publication Date

  • 2010

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